1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/03008207.2020.1828380" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/03008207.2020.1828380</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1-14
ISSN
1607-8438 0300-8207
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1080/03008207.2020.1828380" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1080/03008207.2020.1828380</a>
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Update Year & Number
October 2020 List
NEOMED College
NEOMED College of Medicine
NEOMED College of Pharmacy
NEOMED Department
Department of Anatomy & Neurobiology
Department of Pharmacy Practice
NEOMED Student Publications
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A retrotransposon gag-like-3 gene RTL3 and SOX-9 co-regulate the expression of COL2A1 in chondrocytes.
Publisher
An entity responsible for making the resource available
Connective Tissue Research
Date
A point or period of time associated with an event in the lifecycle of the resource
2020
2020-10-12
Subject
The topic of the resource
gene regulation; chondrogenesis; extracellular matrix; chondrocyte; ZCCHC5/RTL3; RTL3; ZCCHC5
Creator
An entity primarily responsible for making the resource
Ball HC;Ansari M Y;Ahmad N;Novak K;Haqqi TM
Description
An account of the resource
PURPOSE: Transposable elements are known to remodel gene structure and provide a known source of genetic variation. Retrotransposon gag-like-3 (RTL3) is a mammalian retrotransposon-derived transcript (MART) whose function in the skeletal tissue is unknown. This study aimed to elucidate the biological significance of RTL3 in chondrogenesis and type-II collagen (COL2A1) gene expression in chondrocytes. MATERIALS AND METHODS: Expression of RTL3, SOX-9 and COL2A1 mRNAs was determined by TaqMan assays and the protein expression by immunoblotting. RTL3 and Sox-9 depletion in human chondrocytes was achieved using validated siRNAs. An RTL3 mutant (∆RTL3) lacking the zinc finger domain was created using in vitro mutagenesis. Forced expression of RTL3, ∆RTL3, and SOX-9 was achieved using CMV promoter containing expression plasmids. CRISPR-Cas9 was utilized to delete Rtl3 and create a stable ATDC5(Rlt3-/-) cell line. Matrix deposition and Col2a1 quantification during chondrogenesis were determined by Alcian blue staining and Sircol™ Soluble Collagen Assay, respectively. RESULTS: RTL3 is not ubiquitously expressed but showed strong expression in cartilage, chondrocytes and synoviocytes but not in muscle, brain, or other tissues analyzed. Loss-of-function and gain-of-function studies demonstrated a critical role of RTL3 in the regulation of SOX-9 and COL2A1 expression and matrix synthesis during chondrogenesis. Both RTL3 and SOX-9 displayed co-regulated expression in chondrocytes. Gene regulatory activity of RTL3 requires the c-terminal CCHC zinc-finger binding domain. CONCLUSIONS: Our results identify a novel regulatory mechanism of COL2A1 expression in chondrocytes that may help to further understand the skeletal development and the pathogenesis of diseases with altered COL2A1 expression.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/03008207.2020.1828380" target="_blank" rel="noreferrer noopener">10.1080/03008207.2020.1828380</a>
Format
The file format, physical medium, or dimensions of the resource
journalArticle
2020
Ahmad N
Ansari M Y
Ball HC
chondrocyte
chondrogenesis
Connective tissue research
Department of Anatomy & Neurobiology
Department of Pharmacy Practice
Extracellular Matrix
Gene Regulation
Haqqi TM
journalArticle
NEOMED College of Medicine
NEOMED College of Pharmacy
NEOMED Student Publications
Novak K
October 2020 List
RTL3
ZCCHC5
ZCCHC5/RTL3