1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1016/0016-5085(95)90083-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0016-5085(95)90083-7</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 533-544 Issue 2 Volume 108 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource FAILURE OF INTRAVENOUS-INFUSION OF TAUROCHOLATE TO DOWN-REGULATE CHOLESTEROL 7-ALPHA-HYDROXYLASE IN RATS WITH BILIARY FISTULAS Publisher An entity responsible for making the resource available Gastroenterology Date A point or period of time associated with an event in the lifecycle of the resource 1995 1995-02 Subject The topic of the resource 3-hydroxy-3-methylglutaryl; bile-acid synthesis; cholesterol; coenzyme; coenzyme-a reductase; feedback-regulation; Gastroenterology & Hepatology; hepatic; hepatocytes; liquid-chromatography; liver; messenger-rna levels; performance; transcriptional regulation Creator An entity primarily responsible for making the resource Pandak W M; Heuman D M; Hylemon P B; Chiang J Y L; Vlahcevic Z R Description An account of the resource Background/Aims: The decrease in cholesterol 7 alpha-hydroxylase induced by intraduodenal infusion of taurocholate in bile fistula vats may be indirect, i.e., mediated through release or absorption of an intestinal factor in response to the presence of bile salts in the intestine. The aim of this study was to determine if negative feedback regulation of cholesterol 7 alpha-hydroxylase can be shown when equimolar concentrations of taurocholate are administered intravenously, thus bypassing the intestine. Methods: After 96 hours of biliary diversion, taurocholate (36 mu mol.h(-1).100 g rat(-1)) was infused into the rats either intravenously or intraduodenally for the final 24 hours. Livers were then harvested for analysis of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase specific activity, cholesterol 7 alpha-hydroxylase specific activity, messenger RNA levels, and transcriptional activity. Results: Intra-duodenally administered taurocholate significantly decreased HMG-CoA reductase and cholesterol 7 alpha-hydroxyfase specific activity by more than 50% and cholesterol 7 alpha-hydroxylase steady-state messenger RNA levels and transcriptional activity by 50%-75%. In contrast, intravenous administration of taurocholate failed to down-regulate either cholesterol 7 alpha-hydroxylase or HMG-CoA reductase. Conclusions: Passage of taurocholate through the intestine strongly potentiates negative feedback regulation of cholesterol 7 alpha-hydroxylase. A putative intestinal factor, released or absorbed in the presence of bile acids in the intestinal lumen, may play a role in the regulation of bile acid synthesis. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1016/0016-5085(95)90083-7" target="_blank" rel="noreferrer noopener">10.1016/0016-5085(95)90083-7</a> Format The file format, physical medium, or dimensions of the resource Journal Article 1995 3-hydroxy-3-methylglutaryl bile-acid synthesis Chiang J Y L Cholesterol coenzyme coenzyme-a reductase feedback-regulation Gastroenterology Gastroenterology & Hepatology hepatic hepatocytes Heuman D M Hylemon P B Journal Article liquid-chromatography Liver messenger-rna levels Pandak W M Performance transcriptional regulation Vlahcevic Z R