1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1073/pnas.0507467103" target="_blank" rel="noreferrer noopener">http://doi.org/10.1073/pnas.0507467103</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 797-802 Issue 3 Volume 103 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Identification of a receptor-independent activator of G protein signaling (AGS8) in ischemic heart and its interaction with G beta gamma Publisher An entity responsible for making the resource available Proceedings of the National Academy of Sciences of the United States of America Date A point or period of time associated with an event in the lifecycle of the resource 2006 2006-01 Subject The topic of the resource accessory protein; coronary collateral growth; drosophila ric-8; effectors; heterotrimeric g-proteins; hypoxia; localization; modulators; orientation; rat; Science & Technology - Other Topics; signal adaptation; spindle; subunits Creator An entity primarily responsible for making the resource Sato M; Cismowski M J; Toyota E; Smrcka A V; Lucchesi P A; Chilian W M; Lanier S M Description An account of the resource As part of a broader effort to identify postreceptor signal regulators involved in specific diseases or organ adaptation, we used an expression cloning system in Saccharomyces cerevisiae to screen cDNA libraries from rat ischemic myocardium, human heart, and a prostate leiomyosarcoma for entities that activated G protein signaling in the absence of a G protein coupled receptor. We report the characterization of activator of G protein signaling (AGS) 8 (KIAA1866), isolated from a rat heart model of repetitive transient ischemia. AGS8 mRNA was induced in response to ventricular ischemia but not by tachycardia, hypertrophy, or failure. Hypoxia induced AGS8 mRNA in isolated adult ventricular cardiomyocytes but not in rat aortic smooth muscle cells, endothelial cells, or cardiac fibroblasts, suggesting a myocyte-specific adaptation mechanism involving remodeling of G protein signaling pathways. The bioactivity of AGS8 in the yeast-based assay was independent of guanine nucleotide exchange by Ga, suggesting an impact on subunit interactions. Subsequent studies indicated that AGS8 interacts directly with G beta gamma and this occurs in a manner that apparently does not alter the regulation of the effector PLC-beta(2) by G beta gamma. Mechanistically, AGS8 appears to promote G protein signaling by a previously unrecognized mechanism that involves direct interaction with G beta gamma. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1073/pnas.0507467103" target="_blank" rel="noreferrer noopener">10.1073/pnas.0507467103</a> Format The file format, physical medium, or dimensions of the resource Journal Article 2006 accessory protein Chilian W M Cismowski M J coronary collateral growth drosophila ric-8 effectors heterotrimeric g-proteins hypoxia Journal Article Lanier S M localization Lucchesi P A modulators Orientation Proceedings of the National Academy of Sciences of the United States of America rat Sato M Science & Technology - Other Topics signal adaptation Smrcka A V spindle subunits Toyota E