1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
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n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
885-892
Issue
6
Volume
34
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Cholesterol 7-alpha-hydroxylase - Evidence For Transcriptional Regulation By Cholesterol Or Metabolic Products Of Cholesterol In The Rat
Publisher
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Journal of Lipid Research
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-06
Subject
The topic of the resource
bile-acid synthesis; bile fistula; bile-acid synthesis; Biochemistry & Molecular Biology; biosynthesis; cloning; enterohepatic circulation; enzyme; hepatic cholesterol; hepatocytes; hmg-coa reductase; liver microsomes; lovastatin; messenger-rna; mevalonate; stimulation
Creator
An entity primarily responsible for making the resource
Jones M P; Pandak W M; Heuman D M; Chiang J Y L; Hylemon P B; Vlahcevic Z R
Description
An account of the resource
Cholesterol 7alpha-hydroxylase, the rate-determining enzyme in the bile acid biosynthesis pathway, is regulated in a negative feedback manner by hydrophobic bile salts returning to the liver via the portal circulation. The role of cholesterol in the regulation of cholesterol 7alpha-hydroxylase and the interrelationship between the cholesterol and bile acid biosynthesis pathways remain controversial. The objective of the present study was to define the role of cholesterol in the regulation of cholesterol 7alpha-hydroxylase and determine the molecular level of its control. In order to avoid intestinal or intravenous administration of cholesterol, we manipulated the flow of cholesterol within the hepatocytes by decreasing cholesterol synthesis with lovastatin in bile fistula rats (bile acid synthesis is up-regulated), or by increasing cholesterol supply by administering mevalonate, a precursor of cholesterol, to rats with intact enterohepatic circulation (bile acid synthesis is normal). In the first series of studies, lovastatin was administered as a single intravenous bolus (10 mg/kg) to rats with chronic bile fistula and to rats with intact enterohepatic circulation (cholesterol and bile acid synthesis is normal). Three hours after lovastatin administration, cholesterol 7alpha-hydroxylase specific activity, enzyme mass, mRNA, and gene transcriptional activity were decreased by 35%, 32%, 56%, and 34%, respectively, in rats with chronic bile fistula. In rats with intact enterohepatic circulation, lovastatin administration resulted in a similar decrease (34%) of cholesterol 7alpha-hydroxylase specific activity. In the second group of experiments, rats with intact enterohepatic circulation were administered a 180 mum bolus of mevalonate followed by a continuous infusion of 180 mumol/h for 1.5, 3, 4.5, and 24 h prior to being killed. Continuous infusion of mevalonate increased cholesterol 7-alpha-hydroxylase specific activity, mRNA levels, and transcriptional activity by an average of 2- to 3-fold at all time intervals. We conclude that under circumstances in which cholesterol is present in excess, cholesterol 7alpha-hydroxylase transcriptional activity is up-regulated and removal of cholesterol from the hepatocytes is facilitated by an increase of bile acid synthesis. When cholesterol availability is decreased, cholesterol 7alpha-hydroxylase transcriptional activity is down-regulated leading to a decreased elimination of cholesterol via bile acid synthesis. In both instances, hepatic cholesterol homeostasis is effectively maintained.
Identifier
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n/a
Format
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Journal Article or Conference Abstract Publication
1993
bile fistula
bile-acid synthesis
Biochemistry & Molecular Biology
biosynthesis
Chiang J Y L
Cloning
enterohepatic circulation
enzyme
hepatic cholesterol
hepatocytes
Heuman D M
HMG-CoA reductase
Hylemon P B
Jones M P
Journal Article or Conference Abstract Publication
Journal of lipid research
liver microsomes
lovastatin
messenger-rna
mevalonate
Pandak W M
Stimulation
Vlahcevic Z R
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0190-9622(94)70127-x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0190-9622(94)70127-x</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1008-1011
Issue
6
Volume
30
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
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Title
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A PRACTICAL APPROACH TO ANTIBIOTIC-TREATMENT IN WOMEN TAKING ORAL-CONTRACEPTIVES
Publisher
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Journal of the American Academy of Dermatology
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-06
Subject
The topic of the resource
pharmacokinetics; pregnancy; tetracycline; Dermatology; estradiol; rifampicin; ampicillin; drug-interactions; enterohepatic circulation; norethisterone; norgestimate
Creator
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Miller D M; Helms S E; Brodell R T
Description
An account of the resource
Clinical situations that require the use of systemic antibiotic therapy are common. Because millions of women choose oral contraceptives for birth control, the potential for interaction between these drugs frequently has to be considered. We review the available information and present a practical approach for dealing with this situation based on sharing responsibility with an educated patient.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0190-9622(94)70127-x" target="_blank" rel="noreferrer noopener">10.1016/s0190-9622(94)70127-x</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1994
ampicillin
Brodell R T
Department of Family & Community Medicine
Dermatology
drug-interactions
enterohepatic circulation
estradiol
Helms S E
Journal Article or Conference Abstract Publication
Journal of the American Academy of Dermatology
Miller D M
NEOMED College of Medicine
norethisterone
norgestimate
pharmacokinetics
Pregnancy
rifampicin
tetracycline