1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.3390/ijms21165675" target="_blank" rel="noreferrer noopener">http://doi.org/10.3390/ijms21165675</a> Issue 16 Volume 21 ISSN 1422-0067 1422-0067 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.3390/ijms21165675" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.3390/ijms21165675</a> <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Update Year & Number August 2020 List NEOMED College NEOMED College of Medicine NEOMED Department Department of Anatomy & Neurobiology NEOMED Postdoc Publications Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Small non-coding RNAome of ageing chondrocytes. Publisher An entity responsible for making the resource available International Journal of Molecular Sciences Date A point or period of time associated with an event in the lifecycle of the resource 2020 2020-08-07 Subject The topic of the resource chondrocyte; ageing; equine; small non-coding RNA Creator An entity primarily responsible for making the resource Balaskas P; Green JA; Haqqi TM; Dyer P; Kharaz YA; Fang Y; Liu X; Welting TJM; Peffers MJ Description An account of the resource Ageing is a leading risk factor predisposing cartilage to osteoarthritis. However, little research has been conducted on the effect of ageing on the expression of small non-coding RNAs (sncRNAs). RNA from young and old chondrocytes from macroscopically normal equine metacarpophalangeal joints was extracted and subjected to small RNA sequencing (RNA-seq). Differential expression analysis was performed in R using package DESeq2. For transfer RNA (tRNA) fragment analysis, tRNA reads were aligned to horse tRNA sequences using Bowtie2 version 2.2.5. Selected microRNA (miRNAs or miRs) and small nucleolar RNA (snoRNA) findings were validated using real-time quantitative Polymerase Chain Reaction (qRT-PCR) in an extended cohort of equine chondrocytes. tRNA fragments were further investigated in low- and high-grade OA human cartilage tissue. In total, 83 sncRNAs were differentially expressed between young and old equine chondrocytes, including miRNAs, snoRNAs, small nuclear RNAs (snRNAs), and tRNAs. qRT-PCR analysis confirmed findings. tRNA fragment analysis revealed that tRNA halves (tiRNAs), tiRNA-5035-GluCTC and tiRNA-5031-GluCTC-1 were reduced in both high grade OA human cartilage and old equine chondrocytes. For the first time, we have measured the effect of ageing on the expression of sncRNAs in equine chondrocytes. Changes were detected in a number of different sncRNA species. This study supports a role for sncRNAs in ageing cartilage and their potential involvement in age-related cartilage diseases. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.3390/ijms21165675" target="_blank" rel="noreferrer noopener">10.3390/ijms21165675</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Format The file format, physical medium, or dimensions of the resource journalArticle 2020 ageing August 2020 List Balaskas P chondrocyte Department of Anatomy & Neurobiology Dyer P equine Fang Y Green JA Haqqi TM International journal of molecular sciences journalArticle Kharaz YA Liu X NEOMED College of Medicine NEOMED College of Medicine Postdoc NEOMED Postdoc Publications Peffers MJ small non-coding RNA Welting TJM