Innervation of ectopic endometrium in a rat model of endometriosis
pain; neuronal responses; Science & Technology - Other Topics; gene-related peptide; female rats; stimulation; spinal-cord; neuropeptides; lung transplantation; afferent reinnervation; fertility; nerve-endings; transplant; uterus
Endometriosis (ENDO) is a disorder in which vascularized growths of endometrial tissue occur outside the uterus. Its symptoms include reduced fertility and severe pelvic pain. Mechanisms that maintain the ectopic growths and evoke symptoms are poorly understood. One factor not yet considered is that the ectopic growths develop their own innervation. Here, we tested the hypothesis that the growths develop both an autonomic and a sensory innervation. We used a rat model of surgically induced ENDO whose growths mimic those in women. Furthermore, similar to women with ENDO, such rats exhibit reduced fertility and increased pelvic nociception. The ENDO was induced by autotransplanting, on mesenteric cascade arteries, small pieces of uterus that formed vascularized cysts. The cysts and healthy uterus were harvested from proestrous rats and immunostained using the pan-neuronal marker PGP9.5 and specific markers for calcitonin gene-related peptide (CGRP) (sensory C and AS fibers), substance P (SIP) (sensory C and AS fibers) and vesicular monoamine transporter (sympathetic fibers). Cysts (like the uterus) were robustly innervated, with many PGP9.5-stained neurites accompanying blood vessels and extending into nearby luminal epithelial layers. CGRP-, SP-, and vesicular monoamine transporter-immunostained neurites also were observed, with CGRP and SP neurites extending the furthest into the cyst lining. These results demonstrate that ectopic endometrial growths develop an autonomic and sensory innervation. This innervation could contribute not only to symptoms associated with ENDO but also to maintenance of the ectopic growths.
Berkley K J; Dmitrieva N; Curtis K S; Papka R E
Proceedings of the National Academy of Sciences of the United States of America
2004
2004-07
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1073/pnas.0403663101" target="_blank" rel="noreferrer noopener">10.1073/pnas.0403663101</a>
Rat Mesenchymal Stem Cell Secretome Promotes Elastogenesis And Facilitates Recovery From Simulated Childbirth Injury
Cell Biology; Elastin; Electromyography (EMG); expression; External urethral sphincter (EUS); female; female rats; mice; Paracrine factors; protection; Research & Experimental Medicine; Stress urinary incontinence (SUI); stress urinary-incontinence; stromal cells; tissue; transplantation; transplantation; Urethra; urethral sphincter; vaginal distension
Dissaranan C; Cruz M A; Kiedrowski M; Balog B M; Gill B C; Penn M S; Goldman H B; Damaser M S
Cell Transplantation
2014
2014
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.3727/096368913x670921" target="_blank" rel="noreferrer noopener">10.3727/096368913x670921</a>
Estrogen Decreases Corpus Striatal Neurotoxicity In Response To 6-hydroxydopamine
brain; c57/b1 mice; dihydroxyphenylacetic acid; dopamine; dopamine uptake sites; estradiol; female rats; mptp-induced neurotoxicity; neuroprotection; Neurosciences & Neurology; nigrostriatal; ovariectomized rats; parkinsons-disease; parkinsons-disease; progesterone; release
Dluzen D
Brain Research
1997
1997-09
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/s0006-8993(97)00630-6" target="_blank" rel="noreferrer noopener">10.1016/s0006-8993(97)00630-6</a>
Invivo Changes In Responsiveness Of The Caudate-nucleus To L-dopa Infusion As A Function Of The Estrous-cycle
behaviors; caudate nucleus; corpus striatum; dopamine; estrous-cycle; female rats; hormone; in-vitro; l-dihydroxyphenylalanine; Neurosciences & Neurology; progesterone; prolactin; push-pull perfusion; rat striatum; release; sex-differences
Dluzen D E; Ramirez V D
Brain Research
1990
1990-12
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0006-8993(90)90021-3" target="_blank" rel="noreferrer noopener">10.1016/0006-8993(90)90021-3</a>
Estrogen And Parkinson's Disease
alpha-synuclein gene; antiphospholipid antibodies; brain dopamine; dopamine; female rats; gender differences; nigrostriatal dopaminergic system; oral-contraceptives; postmenopausal women; sex-differences; striatal
Horstink Mwim; Strijks E; Dluzen D E
Parkinson's Disease
2003
2003
Book Chapter
n/a