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40
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Text
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<a href="http://doi.org/10.1016/j.cryobiol.2006.10.193" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.cryobiol.2006.10.193</a>
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Pages
36-43
Issue
1
Volume
54
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Title
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Cryopreservation Of Porcine Articular Cartilage: Mri And Biochemical Results After Different Freezing Protocols
Publisher
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Cryobiology
Date
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2007
2007-02
Subject
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2-step procedure; articular-cartilage; biochemical; cryopreservation; culture cells frozen; diffusion; dimethyl sulfoxide; femoral condyles; fresh; Life Sciences & Biomedicine - Other Topics; magnetic-resonance microscopy; MRI; osteoarticular allografts; osteochondral allograft transplantation; Physiology; porcine; survival; tissue
Creator
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Laouar L; Fishbein K; McGann L E; Horton W E; Spencer R G; Jomha N M
Description
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The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups - (1) phosphate buffered saline (PBS) control, (2) PBS snap frozen in liquid nitrogen, (3) slow-cooled in dimethyl sulfoxide (DMSO), and (4) slow cooled in PBS (in absence of DMSO). MRI results demonstrated three distinct zones in the cartilage. After exposure to ice formation during cryopreservation procedures, alterations in MRI determined matrix fixed charged density and magnetization transfer rate were noted. In addition, biochemical assays demonstrated significant alterations in chondroitin sulfate and hydroxyproline content over time without differences in hydration or DNA content. In conclusion, MRI was able to detect some changes in the intact cartilage matrix structure consistent with biochemical assessments after ice formation during cryopreservation of intact porcine AC. Furthermore, biochemical assessments supported some of these findings and changed significantly after incubating the cartilage matrix for 36-72 h in PBS in terms of chondroitin sulfate and hydroxyproline content. (c) 2006 Elsevier Inc. All rights reserved.
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<a href="http://doi.org/10.1016/j.cryobiol.2006.10.193" target="_blank" rel="noreferrer noopener">10.1016/j.cryobiol.2006.10.193</a>
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Journal Article or Conference Abstract Publication
2-step procedure
2007
articular-cartilage
biochemical
Cryobiology
cryopreservation
culture cells frozen
diffusion
dimethyl sulfoxide
femoral condyles
Fishbein K
fresh
Horton W E
Jomha N M
Journal Article or Conference Abstract Publication
Laouar L
Life Sciences & Biomedicine - Other Topics
magnetic-resonance microscopy
McGann L E
MRI
osteoarticular allografts
osteochondral allograft transplantation
Physiology
porcine
Spencer R G
Survival
tissue