1
40
3
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1371/journal.pone.0035831" target="_blank" rel="noreferrer noopener">http://doi.org/10.1371/journal.pone.0035831</a>
Pages
e35831–e35831
Issue
4
Volume
7
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Development of GPCR modulation of GABAergic transmission in chicken nucleus laminaris neurons.
Publisher
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PloS one
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
1905-07
Subject
The topic of the resource
Animals; Patch-Clamp Techniques; *Synaptic Transmission; gamma-Aminobutyric Acid/*metabolism; Neurons/*metabolism; Brain/*cytology; Chickens/*metabolism; Synapses/metabolism; Receptors; G-Protein-Coupled/*metabolism; GABA-A/metabolism; GABA-B/metabolism; Metabotropic Glutamate/metabolism
Creator
An entity primarily responsible for making the resource
Tang Zheng-Quan; Lu Yong
Description
An account of the resource
Neurons in the nucleus laminaris (NL) of birds act as coincidence detectors and encode interaural time difference to localize the sound source in the azimuth plane. GABAergic transmission in a number of CNS nuclei including the NL is subject to a dual modulation by presynaptic GABA(B) receptors (GABA(B)Rs) and metabotropic glutamate receptors (mGluRs). Here, using in vitro whole-cell patch clamp recordings from acute brain slices of the chick, we characterized the following important but unknown properties pertaining to such a dual modulation: (1) emergence of functional GABA synapses in NL neurons; (2) the temporal onset of neuromodulation mediated by GABA(B)Rs and mGluRs; and (3) the physiological conditions under which GABA(B)Rs and mGluRs are activated by endogenous transmitters. We found that (1) GABA(A)R-mediated synaptic responses were observed in about half of the neurons at embryonic day 11 (E11); (2) GABA(B)R-mediated modulation of the GABAergic transmission was detectable at E11, whereas the modulation by mGluRs did not emerge until E15; and (3) endogenous activity of GABA(B)Rs was induced by both low- (5 or 10 Hz) and high-frequency (200 Hz) stimulation of the GABAergic pathway, whereas endogenous activity of mGluRs was induced by high- (200 Hz) but not low-frequency (5 or 10 Hz) stimulation of the glutamatergic pathway. Furthermore, the endogenous activity of mGluRs was mediated by group II but not group III members. Therefore, autoreceptor-mediated modulation of GABAergic transmission emerges at the same time when the GABA synapses become functional. Heteroreceptor-mediated modulation appears at a later time and is receptor type dependent in vitro.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1371/journal.pone.0035831" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0035831</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Synaptic Transmission
2012
Animals
Brain/*cytology
Chickens/*metabolism
Department of Anatomy & Neurobiology
G-Protein-Coupled/*metabolism
GABA-A/metabolism
GABA-B/metabolism
gamma-Aminobutyric Acid/*metabolism
Lu Yong
Metabotropic Glutamate/metabolism
NEOMED College of Medicine
Neurons/*metabolism
Patch-Clamp Techniques
PloS one
Receptors
Synapses/metabolism
Tang Zheng-Quan
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuroscience.2013.01.061" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuroscience.2013.01.061</a>
Pages
170–183
Volume
237
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Analysis of excitatory synapses in the guinea pig inferior colliculus: a study using electron microscopy and GABA immunocytochemistry.
Publisher
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Neuroscience
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-05
Subject
The topic of the resource
*Inferior Colliculi/cytology/metabolism/ultrastructure; *Microscopy; Animals; Female; gamma-Aminobutyric Acid/*metabolism; Guinea Pigs; Immunoelectron; Male; NADPH Dehydrogenase/metabolism; Neurons/metabolism/*ultrastructure; Presynaptic Terminals/metabolism/ultrastructure; Synapses/*physiology/*ultrastructure
Creator
An entity primarily responsible for making the resource
Nakamoto K T; Mellott J G; Killius J; Storey-Workley M E; Sowick C S; Schofield B R
Description
An account of the resource
The inferior colliculus (IC) integrates ascending auditory input from the lower brainstem and descending input from the auditory cortex. Understanding how IC cells integrate these inputs requires identification of their synaptic arrangements. We describe excitatory synapses in the dorsal cortex, central nucleus, and lateral cortex of the IC (ICd, ICc and IClc) in guinea pigs. We used electron microscopy (EM) and post-embedding anti-GABA immunogold histochemistry on aldehyde-fixed tissue from pigmented adult guinea pigs. Excitatory synapses were identified by round vesicles, asymmetric synaptic junctions, and gamma-aminobutyric acid-immunonegative (GABA-negative) presynaptic boutons. Excitatory synapses constitute approximately 60% of the synapses in each IC subdivision. Three types can be distinguished by presynaptic profile area and number of mitochondrial profiles. Large excitatory (LE) boutons are more than 2 mum(2) in area and usually contain five or more mitochondrial profiles. Small excitatory (SE) boutons are usually less than 0.7 mum(2) in area and usually contain 0 or 1 mitochondria. Medium excitatory (ME) boutons are intermediate in size and usually contain 2 to 4 mitochondria. LE boutons are mostly confined to the ICc, while the other two types are present throughout the IC. Dendritic spines are the most common target of excitatory boutons in the IC dorsal cortex, whereas dendritic shafts are the most common target in other IC subdivisions. Finally, each bouton type terminates on both gamma-aminobutyric acid-immunopositive (GABA+) and GABA-negative (i.e., glutamatergic) targets, with terminations on GABA-negative profiles being much more frequent. The ultrastructural differences between the three types of boutons presumably reflect different origins and may indicate differences in postsynaptic effect. Despite such differences in origins, each of the bouton types contact both GABAergic and non-GABAergic IC cells, and could be expected to activate both excitatory and inhibitory IC circuits.
Identifier
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<a href="http://doi.org/10.1016/j.neuroscience.2013.01.061" target="_blank" rel="noreferrer noopener">10.1016/j.neuroscience.2013.01.061</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Inferior Colliculi/cytology/metabolism/ultrastructure
*Microscopy
2013
Animals
Department of Anatomy & Neurobiology
Female
gamma-Aminobutyric Acid/*metabolism
Guinea Pigs
Immunoelectron
Killius J
Male
Mellott J G
NADPH Dehydrogenase/metabolism
Nakamoto K T
NEOMED College of Medicine
Neurons/metabolism/*ultrastructure
Neuroscience
Presynaptic Terminals/metabolism/ultrastructure
Schofield B R
Sowick C S
Storey-Workley M E
Synapses/*physiology/*ultrastructure
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuroscience.2009.09.013" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuroscience.2009.09.013</a>
Pages
1009–1019
Issue
3
Volume
164
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of glutamatergic and GABAergic neurotransmission in the chick nucleus laminaris: role of N-type calcium channels.
Publisher
An entity responsible for making the resource available
Neuroscience
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-12
Subject
The topic of the resource
Animals; Auditory Pathways/cytology/drug effects/metabolism; Calcium Channel Blockers/pharmacology; Calcium Channels; Chick Embryo; Chickens; Cochlear Nucleus/cytology/drug effects/*metabolism; Excitatory Postsynaptic Potentials/drug effects/physiology; Functional Laterality/drug effects/physiology; gamma-Aminobutyric Acid/*metabolism; Glutamic Acid/*metabolism; N-Type/*metabolism; Neural Inhibition/physiology; Neurons/drug effects/metabolism; omega-Conotoxin GVIA/pharmacology; Organ Culture Techniques; Patch-Clamp Techniques; Rhombencephalon/cytology/*metabolism; Synaptic Transmission/drug effects/*physiology
Creator
An entity primarily responsible for making the resource
Lu Y
Description
An account of the resource
Neurons in the chicken nucleus laminaris (NL), the third order auditory nucleus involved in azimuth sound localization, receive bilaterally segregated (ipsilateral vs contralateral) glutamatergic excitation from the cochlear nucleus magnocellularis and GABAergic inhibition from the ipsilateral superior olivary nucleus (SON). Here, I investigate the voltage-gated calcium channels (VGCCs) that trigger the excitatory and the inhibitory transmission in the NL. Whole-cell recordings were performed in acute brainstem slices. The excitatory transmission was predominantly mediated by N-type VGCCs, as the specific N-type blocker omega-Conotoxin-GVIA (omega-CTx-GVIA, 1-2.5 microM) inhibited excitatory postsynaptic currents (EPSCs) by approximately 90%. Blockers for P/Q- and L-type VGCCs produced no inhibition, and blockade of R-type VGCCs produced a small inhibition. In individual cells, the effect of each VGCC blocker on the EPSC elicited by activation of the ipsilateral input was the same as that on the EPSC elicited by activation of the contralateral input, and the two EPSCs had similar kinetics, suggesting physiological symmetry between the two glutamatergic inputs to single NL neurons. The inhibitory transmission in NL neurons was almost exclusively mediated by N-type VGCCs, as omega-CTx-GVIA (1 microM) produced a approximately 90% reduction of inhibitory postsynaptic currents, whereas blockers for other VGCCs produced no inhibition. In conclusion, N-type VGCCs play a dominant role in triggering both the excitatory and the inhibitory transmission in the NL, and the presynaptic VGCCs that mediate the two bilaterally segregated glutamatergic inputs to individual NL neurons are identical. These features may play a role in optimizing coincidence detection in NL neurons.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.neuroscience.2009.09.013" target="_blank" rel="noreferrer noopener">10.1016/j.neuroscience.2009.09.013</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Animals
Auditory Pathways/cytology/drug effects/metabolism
Calcium Channel Blockers/pharmacology
Calcium Channels
Chick Embryo
Chickens
Cochlear Nucleus/cytology/drug effects/*metabolism
Department of Anatomy & Neurobiology
Excitatory Postsynaptic Potentials/drug effects/physiology
Functional Laterality/drug effects/physiology
gamma-Aminobutyric Acid/*metabolism
Glutamic Acid/*metabolism
Lu Y
N-Type/*metabolism
NEOMED College of Medicine
Neural Inhibition/physiology
Neurons/drug effects/metabolism
Neuroscience
omega-Conotoxin GVIA/pharmacology
Organ Culture Techniques
Patch-Clamp Techniques
Rhombencephalon/cytology/*metabolism
Synaptic Transmission/drug effects/*physiology