1
40
3
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Text
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URL Address
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-1119-7_15"><span>https://doi.org/10.1007/978-1-0716-1119-7_15</span></a>
ISSN
978-1-0716-1119-7
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Update Year & Number
Jan to Aug list 2021
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.
Creator
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Ansari MY; Haqqi TM
Publisher
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Methods in Molecular Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2020
2020-12-15
Subject
The topic of the resource
Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines.
Identifier
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<a href="https://link.springer.com/protocol/10.1007/978-1-0716-1119-7_15"><span>https://doi.org/10.1007/978-1-0716-1119-7_15</span></a>
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© Springer Science+Business Media, LLC, part of Springer Nature 2021
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2020
Autophagy
CCCP
Chondrocytes
Confocal microscopy
IL-1β
immunofluorescence
Inflammation
Mitochondria
Mitochondrial dysfunction
mitophagy
MitoTracker Deep Red
Osteoarthritis
Oxidative Stress
Parkin
PINK1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/978-1-0716-1119-7_15</a>
Pages
215-224
Volume
2245
ISSN
1940-6029 1064-3745
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1007/978-1-0716-1119-7_15</a>
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Update Year & Number
January 2021 List
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.
Publisher
An entity responsible for making the resource available
Methods in Molecular Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
1905-07
Subject
The topic of the resource
Inflammation; Mitochondria; Oxidative stress; Osteoarthritis; Chondrocytes; Inflammation; Osteoarthritis; Autophagy; CCCP; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Parkin; PINK1; Autophagy; CCCP; Chondrocytes; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondria; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Oxidative stress; Parkin; PINK1
Creator
An entity primarily responsible for making the resource
Ansari MY; Haqqi TM
Description
An account of the resource
Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">10.1007/978-1-0716-1119-7_15</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Format
The file format, physical medium, or dimensions of the resource
journalArticle
2021
Ansari MY
Autophagy
CCCP
Chondrocytes
Confocal microscopy
Department of Anatomy & Neurobiology
Haqqi TM
IL-1β
immunofluorescence
Inflammation
January 2021 List
journalArticle
Methods in Molecular Biology
Mitochondria
Mitochondrial dysfunction
mitophagy
MitoTracker Deep Red
NEOMED College of Medicine
Osteoarthritis
Oxidative Stress
Parkin
PINK1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/08860220802213062" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/08860220802213062</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
759-762
Issue
7
Volume
30
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Fibrillary glomerulonephritis with hepatitis C viral infection and hypocomplementemia
Publisher
An entity responsible for making the resource available
Renal Failure
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008
Subject
The topic of the resource
deposits; entity; features; fibrillary glomerulonephritis; hepatitis C infection; hypocomplementemia; immunofluorescence; immunotactoid glomerulopathy; Urology & Nephrology
Creator
An entity primarily responsible for making the resource
Ray S; Rouse K; Appis A; Novak R; Haller N A
Description
An account of the resource
Fibrillary glomerulonephritis (FGN) is a relatively rare cause of renal disease, found in only 0.6-1.5% of native renal biopsies. The pathogenesis of FGN is not well described, and very few associations with disease processes other than hepatitis C virus (HCV) have been made. We describe a case that provides evidence in support of the FGN-HCV association, as well as introduces the association of FGN-HCV and hypocomplementemia. The case is a 53-year-old African-American female demonstrating a classical presentation of FGN complicated by a concomitant HCV infection. Treating an HCV infection with alpha-interferon has been shown to result in subsequent improvement in the nephrotic syndrome and renal function. However, this patient is unique in that she is complicated with hypocomplementemia, creating a complex treatment situation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/08860220802213062" target="_blank" rel="noreferrer noopener">10.1080/08860220802213062</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2008
Appis A
deposits
entity
features
fibrillary glomerulonephritis
Haller N A
hepatitis C infection
hypocomplementemia
immunofluorescence
immunotactoid glomerulopathy
Journal Article
Novak R
Ray S
Renal failure
Rouse K
Urology & Nephrology