1
40
33
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/nar/27.10.2219" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/nar/27.10.2219</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
2219-2226
Issue
10
Volume
27
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Oxidative damage of DNA by chromium(V) complexes: relative importance of base versus sugar oxidation
Publisher
An entity responsible for making the resource available
Nucleic Acids Research
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-05
Subject
The topic of the resource
Biochemistry & Molecular Biology; in-vitro; nucleic-acids; abstraction; aqueous-solution; carcinogen chromium(vi); hydrogen; molecular-oxygen; radical formation; redox potentials; singlet oxygen; strand breaks
Creator
An entity primarily responsible for making the resource
Bose R N; Moghaddas S; Mazzer P A; Dudones L P; Joudah L; Stroup D
Description
An account of the resource
Chromium(V)-mediated oxidative damage of deoxyribonucleic acids was investigated at neutral pH in aqueous solution by utilizing bis(2-ethyl-2-hydroxybutanato)oxochromate(V) (I) and bis(hydroxyethyl)amino-tris(hydroxymethyl)methane)oxochromate(V) (II). Single-stranded and double-stranded (ds) calf thymus and human placenta DNA, as well as two oligomers, 5'-GATCTAGTAGGAGGACAAATAGTGTPTG-3' and 5'-GATCCAAGCAAACACTATTTGTCCTCCTACTA-3', were reacted with the chromium(V) complexes. Most products were separated and characterized by chromatographic and spectroscopic methods, Polyacrylamide gel electrophoresis experiments reveal more damage at G sites in comparison to other bases. Three primary oxidation products, 5-methylene-2-furanone (5-MF), furfural and 8-oxo-2'-deoxyguanosine, were characterized, A minor product, which appears to be thymine propenal, was also observed. The dsDNA produces more furfural than furanone, The formation of these two products resulted from hydrogen abstraction dr hydride transfer from C1' and C5' positions of the ribose to the oxo-chromium(V) center. Since no enhancements of these products (except propenal) were observed in the presence of oxygen, mechanisms pertaining to the participation of activated oxygen species may be ruled out. The oxidation of the G base is most likely associated with an oxygen atom transfer from the oxo-metallates to the double bond between C8 and N7 of the purine ring. The formation of the propenal may be associated with an oxygen-activated species, since a marginal enhancement of this product was observed in the presence of oxygen, The formation of furfural in higher abundance over 5-MF for dsDNA was attributed to the ease of hydrogen abstraction (or hydride transfer) from the C5' compared to C1' position of the ribose within a Cr(V)-DNA intermediate in which the metal center is bound to the phosphate diester moiety.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/nar/27.10.2219" target="_blank" rel="noreferrer noopener">10.1093/nar/27.10.2219</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1999
abstraction
aqueous-solution
Biochemistry & Molecular Biology
Bose R N
carcinogen chromium(vi)
Dudones L P
hydrogen
in-vitro
Joudah L
Journal Article or Conference Abstract Publication
Mazzer P A
Moghaddas S
molecular-oxygen
Nucleic acids research
nucleic-acids
radical formation
redox potentials
singlet oxygen
strand breaks
Stroup D
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1182/blood-2007-10-118497" target="_blank" rel="noreferrer noopener">http://doi.org/10.1182/blood-2007-10-118497</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
256-263
Issue
2
Volume
112
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Dublin Core
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Title
A name given to the resource
Scavenging roles of chemokine receptors: chemokine receptor deficiency is associated with increased levels of ligand in circulation and tissues
Publisher
An entity responsible for making the resource available
Blood
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008-07
Subject
The topic of the resource
in-vitro; central-nervous-system; Hematology; blood-brain-barrier; ccx-ckr; cutting edge; d6; dendritic cell; experimental autoimmune encephalomyelitis; immune-response; sjogrens-syndrome
Creator
An entity primarily responsible for making the resource
Cardona A E; Sasse M E; Liu L P; Cardona S M; Mizutani M; Savarin C; Hu T; Ransohoff R M
Description
An account of the resource
In vitro studies have implicated chemokine receptors in consumption and clearance of specific ligands. We studied the role that various signaling chemokine receptors play during ligand homeostasis in vivo. We examined the levels of ligands in serum and CNS tissue in mice lacking chemokine receptors. Compared with receptor-sufficient controls, Cx3cr1(-/-) mice exhibited augmented levels of CX3CL1 both in serum and brain, and circulating levels of CXCL1 and CXCL2 were increased in Cxcr2(-/-) mice. CCR2-deficient mice showed significantly increased amounts of circulating CCL2 compared with wild-type mice. Cxcr3(-/-) mice revealed increased levels of circulating and brain CXCL10 after experimental autoimmune encephalomyelitis (EAE) induction. CCR2-deficient peripheral blood and resident peritoneal cells exhibited reduced binding capacity and biologic responses to the CCR1 ligand CCL3, suggesting that elevated levels of CCR2 ligands had down-regulated CCR1. The results indicate that signaling chemokine receptors clear chemokines from circulation and tissues. These homeostatic functions of signaling chemokine receptors need to be integrated into safety and efficacy calculations when considering therapeutic receptor blockade.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1182/blood-2007-10-118497" target="_blank" rel="noreferrer noopener">10.1182/blood-2007-10-118497</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2008
Blood
blood-brain-barrier
Cardona A E
Cardona S M
ccx-ckr
central-nervous-system
cutting edge
d6
dendritic cell
experimental autoimmune encephalomyelitis
Hematology
Hu T
immune-response
in-vitro
Journal Article or Conference Abstract Publication
Liu L P
Mizutani M
Ransohoff R M
Sasse M E
Savarin C
sjogrens-syndrome
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bpj.2010.04.011" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bpj.2010.04.011</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
115-123
Issue
1
Volume
99
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Title
A name given to the resource
Actomyosin Tension Exerted on the Nucleus through Nesprin-1 Connections Influences Endothelial Cell Adhesion, Migration, and Cyclic Strain-Induced Reorientation
Publisher
An entity responsible for making the resource available
Biophysical Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-07
Subject
The topic of the resource
cytoskeleton; Biophysics; in-vitro; actin; envelope; polarization; dreifuss muscular-dystrophy; focal adhesions; lamin-a/c; mechanical stiffness; membrane protein; shape
Creator
An entity primarily responsible for making the resource
Chancellor T J; Lee J; Thodeti C K; Lele T
Description
An account of the resource
Endothelial cell polarization and directional migration is required for angiogenesis. Polarization and motility requires not only local cytoskeletal remodeling but also the motion of intracellular organelles such as the nucleus. However, the physiological significance of nuclear positioning in the endothelial cell has remained largely unexplored. Here, we show that siRNA knockdown of nesprin-1, a protein present in the linker of nucleus to cytoskeleton complex, abolished the reorientation of endothelial cells in response to cyclic strain. Confocal imaging revealed that the nuclear height is substantially increased in nesprin-1 depleted cells, similar to myosin inhibited cells. Nesprin-1 depletion increased the number of focal adhesions and substrate traction while decreasing the speed of cell migration; however, there was no detectable change in nonmuscle myosin II activity in nesprin-1 deficient cells. Together, these results are consistent with a model in which the nucleus balances a portion of the actomyosin tension in the cell. In the absence of nesprin-1, actomyosin tension is balanced by the substrate, leading to abnormal adhesion, migration, and cyclic strain-induced reorientation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.bpj.2010.04.011" target="_blank" rel="noreferrer noopener">10.1016/j.bpj.2010.04.011</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2010
actin
Biophysical Journal
Biophysics
Chancellor T J
cytoskeleton
dreifuss muscular-dystrophy
envelope
Focal Adhesions
in-vitro
Journal Article or Conference Abstract Publication
lamin-a/c
Lee J
Lele T
mechanical stiffness
membrane protein
polarization
shape
Thodeti C K
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpheart.00019.2011" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpheart.00019.2011</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
H270-H277
Issue
1
Volume
302
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Title
A name given to the resource
Optical mapping of cryoinjured rat myocardium grafted with mesenchymal stem cells
Publisher
An entity responsible for making the resource available
American Journal of Physiology-Heart and Circulatory Physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-01
Subject
The topic of the resource
cardiomyocytes; Myocardial infarction; therapy; Physiology; Cardiovascular System & Cardiology; in-vitro; repair; Transplantation; phenotype; regeneration; infarction; heart; stem cell; action potential; pacemakers
Creator
An entity primarily responsible for making the resource
Costa A R; Panda N C; Yong S; Mayorga M E; Pawlowski G P; Fan K K; Penn M S; Laurita K R
Description
An account of the resource
Costa AR, Panda NC, Yong S, Mayorga ME, Pawlowski GP, Fan K, Penn MS, Laurita KR. Optical mapping of cryoinjured rat myocardium grafted with mesenchymal stem cells. Am J Physiol Heart Circ Physiol 302: H270-H277, 2012. First published October 28, 2011; doi: 10.1152/ajpheart.00019.2011.-Mesenchymal stem cells (MSCs) have been shown to improve cardiac electrophysiology when administered in the setting of acute myocardial infarction. However, the electrophysiological phenotype of MSCs in situ is not clear. We hypothesize that MSCs delivered intramyocardially to cryoinjured myocardium can engraft, but will not actively generate, action potentials. Cryoinjury-induced scar was created on the left ventricular epicardial surface of adult rat hearts. Within 30 min, hearts were injected with saline (sham, n = 11) or bone marrow-derived MSCs (2 x 10(6)) labeled with 1,1'-dioctadecyl-3,3,3,3'-tetramethyl-indocarbocyanine percholate (DiI; n = 16). At 3 wk, optical mapping and cell isolation were used to measure optical action potentials and calcium transients, respectively. Histological analysis confirmed subepicardial scar thickness and the presence of DiI-positive cells that express connexin-43. Optical action potential amplitude within the scar at MSC-positive sites (53.8 +/- 14.3%) was larger compared with sites devoid of MSCs (35.3 +/- 14.2%, P < 0.05) and sites within the scar of shams (33.5 +/- 6.9%, P < 0.05). Evidence of simultaneous action potential upstroke, the loss of action potential activity following ablation of adjacent viable myocardium, and no rapid calcium transient response in isolated DiI + cells suggest that the electrophysiological influence of engrafted MSCs is electrotonic. MSCs can engraft when directly injected into a cryoinjury and are associated with evidence of action potential activity. However, our results suggest that this activity is not due to generation of action potentials, but rather passive influence coupled from neighboring viable myocardium.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpheart.00019.2011" target="_blank" rel="noreferrer noopener">10.1152/ajpheart.00019.2011</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2012
action potential
American Journal of Physiology-Heart and Circulatory Physiology
cardiomyocytes
Cardiovascular System & Cardiology
Costa A R
Fan K K
heart
in-vitro
Infarction
Journal Article or Conference Abstract Publication
Laurita K R
Mayorga M E
myocardial infarction
pacemakers
Panda N C
Pawlowski G P
Penn M S
Phenotype
Physiology
Regeneration
repair
stem cell
therapy
Transplantation
Yong S
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0006-8993(90)90021-3" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0006-8993(90)90021-3</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
163-168
Issue
1
Volume
536
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Invivo Changes In Responsiveness Of The Caudate-nucleus To L-dopa Infusion As A Function Of The Estrous-cycle
Publisher
An entity responsible for making the resource available
Brain Research
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-12
Subject
The topic of the resource
behaviors; caudate nucleus; corpus striatum; dopamine; estrous-cycle; female rats; hormone; in-vitro; l-dihydroxyphenylalanine; Neurosciences & Neurology; progesterone; prolactin; push-pull perfusion; rat striatum; release; sex-differences
Creator
An entity primarily responsible for making the resource
Dluzen D E; Ramirez V D
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0006-8993(90)90021-3" target="_blank" rel="noreferrer noopener">10.1016/0006-8993(90)90021-3</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1990
behaviors
Brain research
caudate nucleus
corpus striatum
Dluzen D E
Dopamine
estrous-cycle
female rats
hormone
in-vitro
l-dihydroxyphenylalanine
Neurosciences & Neurology
progesterone
prolactin
push-pull perfusion
Ramirez V D
rat striatum
release
sex-differences
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
85-88
Issue
2
Volume
10
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Role Of Urokinase Pais In The Control Of Cancer Invasion And Metastasis
Publisher
An entity responsible for making the resource available
Drug News & Perspectives
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-03
Subject
The topic of the resource
adenocarcinomas; angiogenesis; cells; degradation; inhibition; in-vitro; migration; Pharmacology & Pharmacy; plasminogen-activator; receptor; tumor-metastasis
Creator
An entity primarily responsible for making the resource
Evans D M; Sloan-Stakleff K
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1997
adenocarcinomas
angiogenesis
Cells
degradation
Drug News & Perspectives
Evans D M
in-vitro
inhibition
migration
Pharmacology & Pharmacy
plasminogen-activator
Receptor
Sloan-Stakleff K
tumor-metastasis
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0006-8993(95)00568-b" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0006-8993(95)00568-b</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
189-196
Issue
2
Volume
689
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Spermine Increases Paired-pulse Facilitation In Area Ca1 Of Hippocampus In A Calcium-dependent Manner
Publisher
An entity responsible for making the resource available
Brain Research
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-08
Subject
The topic of the resource
activation; binding; ca1; ca2+ channel; channels; field potential; hippocampal slice; in-vitro; ischemia; Neurosciences & Neurology; ornithine decarboxylase; paired-pulse facilitation; polyamine; polyamines; pyramidal cells; rat-brain; slices; spermine
Creator
An entity primarily responsible for making the resource
Ferchmin P A; Eterovic V A; Rivera E M; Teyler T J
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0006-8993(95)00568-b" target="_blank" rel="noreferrer noopener">10.1016/0006-8993(95)00568-b</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1995
activation
Binding
Brain research
ca1
ca2+ channel
channels
Eterovic V A
Ferchmin P A
field potential
hippocampal slice
in-vitro
ischemia
Neurosciences & Neurology
ornithine decarboxylase
paired-pulse facilitation
polyamine
polyamines
pyramidal cells
rat-brain
Rivera E M
slices
spermine
Teyler T J
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1097/00019048-199711002-00006" target="_blank" rel="noreferrer noopener">http://doi.org/10.1097/00019048-199711002-00006</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
S59-S66
Volume
6
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Fluoroquinolones And Respiratory Tract Infections: Do They Work?
Publisher
An entity responsible for making the resource available
Infectious Diseases in Clinical Practice
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-11
Subject
The topic of the resource
acute; antibacterial activity; antibiotic-therapy; chronic-bronchitis; community-acquired pneumonia; exacerbations; hospitalization; Immunology; in-vitro; Infectious Diseases; obstructive pulmonary-disease; oral ciprofloxacin; requiring; streptococcus-pneumoniae
Creator
An entity primarily responsible for making the resource
File T M
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1097/00019048-199711002-00006" target="_blank" rel="noreferrer noopener">10.1097/00019048-199711002-00006</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1997
acute
antibacterial activity
antibiotic-therapy
chronic-bronchitis
Community-acquired pneumonia
exacerbations
File T M
Hospitalization
Immunology
in-vitro
Infectious Diseases
Infectious Diseases in Clinical Practice
obstructive pulmonary-disease
oral ciprofloxacin
requiring
streptococcus-pneumoniae
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1186/1475-2840-11-150" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/1475-2840-11-150</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
11-11
Volume
11
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Detecting Dna Synthesis Of Neointimal Formation After Catheter Balloon Injury In Gk And In Wistar Rats: Using 5-ethynyl-2 '-deoxyuridine
Publisher
An entity responsible for making the resource available
Cardiovascular Diabetology
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-12
Subject
The topic of the resource
alkynes; arterial injury; balloon injury; Cardiovascular System & Cardiology; carotid-artery; Catheter; cell-cycle progression; click chemistry; diabetes-mellitus; diabetes-mellitus; DNA synthesis; EdU; eluting stents; Endocrinology & Metabolism; in-vitro; mammalian target; Neointimal formation; PCNA; proliferation; terminal
Creator
An entity primarily responsible for making the resource
Guo J S; Li D Y; Bai S R; Xu T D; Zhou Z M; Zhang Y B
Description
An account of the resource
Background: Neointimal formation plays an important role in the pathogenesis of coronary restenosis after percutaneous coronary intervention (PCI), especially in patients with diabetes mellitus. Recently, some studies have shown that 5-ethynyl-2'-deoxyuridine (EdU) incorporation can serve as a novel alternative to the 5-bromo-2'-deoxyuridine (BrdU) antibody detection method for detection of DNA synthesis in regenerating avian cochlea, chick embryo and the adult nervous system. However, few studies have been performed to assess the suitability of EdU for detecting DNA synthesis in vascular neointima. Methods: The carotid artery balloon injury model was established in Goto-Kakizaki (GK) and Wistar rats. A Cell-Light (TM) EdU Kit was used to detect EdU-labeled cell nuclei of common carotid arteries at day 7 after catheter balloon injury. Different methods of injecting EdU were tested. The protein levels of proliferating cell nuclear antigen (PCNA) and p-Akt (Ser473), as well as the mRNA levels of PCNA were evaluated by Western blotting and quantitative real-time PCR (qRT-PCR), respectively. Immunohistochemical staining was also employed to visualize PCNA-positive cells. Results: At day 7 after catheter balloon injury, far more EdU-positive and PCNA-positive cells were observed in GK rats. When comparing groups that received different EdU doses, it was found that the percentage of EdU-positive cells at a dose of 100 mg/kg body weight was than at doses of 25 mg/kg and 50 mg/kg. The number of positive cells was significantly higher in the repeated injection group compared to the single injection group. Further, after balloon injury DNA synthesis in GK rats was more notable than in Wistar rats. Neointimal formation in GK rats was more obvious than in Wistar rats. The protein levels of PCNA and p-Akt (Ser473) and the mRNA levels of PCNA were increased in injured rats as compared to uninjured rats, and were significantly higher in GK rats than in Wistar rats. Conclusion: By intraperitoneal injections of EdU at a dose of 100 mg/kg three times, EdU incorporation can detect carotid arterial DNA synthesis caused by neointimal formation in GK rats and Wistar rats at day 7 after balloon injury by the EdU click reaction quickly and effectively. Moreover, more obvious DNA synthesis in the vascular neointima could be observed in GK rats than in Wistar rats.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1186/1475-2840-11-150" target="_blank" rel="noreferrer noopener">10.1186/1475-2840-11-150</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2012
alkynes
arterial injury
Bai S R
balloon injury
Cardiovascular Diabetology
Cardiovascular System & Cardiology
carotid-artery
Catheter
cell-cycle progression
click chemistry
diabetes-mellitus
DNA synthesis
EdU
eluting stents
Endocrinology & Metabolism
Guo J S
in-vitro
Journal Article or Conference Abstract Publication
Li D Y
mammalian target
Neointimal formation
PCNA
proliferation
terminal
Xu T D
Zhang Y B
Zhou Z M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.joca.2010.01.006" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.joca.2010.01.006</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
724-733
Issue
5
Volume
18
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Modification Of Osteoarthritis In The Guinea Pig With Pulsed Low-intensity Ultrasound Treatment
Publisher
An entity responsible for making the resource available
Osteoarthritis and Cartilage
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-05
Subject
The topic of the resource
aggrecan; articular-cartilage; cartilage; Cartilage repair; degeneration; double-blind trial; electromagnetic-fields; gene-expression; in-vitro; knee osteoarthritis; matrix production; Orthopedics; osteoarthritis; placebo-controlled trials; Rheumatology; therapeutic ultrasound; tissue engineered cartilage; Ultrasound
Creator
An entity primarily responsible for making the resource
Gurkan I; Ranganathan A; Yang X; Horton W E; Todman M; Huckle J; Pleshko N; Spencer R G
Description
An account of the resource
Objective: The Hartley guinea pig develops articular cartilage degeneration similar to that seen in idiopathic human osteoarthritis (OA). We investigated whether the application of pulsed low-intensity ultrasound (PLIUS) to the Hartley guinea pig joint would prevent or attenuate the progression of this degenerative process. Methods: Treatment of male Hartley guinea pigs was initiated at the onset of degeneration (8 weeks of age) to assess the ability of PLIUS to prevent OA, or at a later age (12 months) to assess the degree to which PLIUS acted to attenuate the progression of established disease. PLIUS (30 mW/cm(2)) was applied to stifle joints for 20 min/day over periods ranging from 3 to 10 months, with contralateral limbs serving as controls. Joint cartilage histology was graded according to a modified Mankin scale to evaluate treatment effect. Immunohistochemical staining for interleukin-1 receptor antagonist (IL-1ra), matrix metalloproteinase (MMP)-3, MMP-13, and transforming growth factor (TGF)-beta 1 was performed on the cartilage to evaluate patterns of expression of these proteins. Results: PLIUS did not fully prevent cartilage degeneration in the prevention groups, but diminished the severity of the disease, with the treated joints showing markedly decreased surface irregularities and a much smaller degree of loss of matrix staining as compared to controls. PLIUS also attenuated disease progression in the groups with established disease, although to a somewhat lesser extent as compared to the prevention groups. Immunohistochemical staining demonstrated a markedly decreased degree of TGF-beta 1 production in the PLIUS-treated joints. This indicates less active endogenous repair, consistent with the marked reduction in cartilage degradation. Conclusions: PLIUS exhibits the ability to attenuate the progression of cartilage degeneration in an animal model of idiopathic human OA. The effect was greater in the treatment of early, rather than established, degeneration. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.joca.2010.01.006" target="_blank" rel="noreferrer noopener">10.1016/j.joca.2010.01.006</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2010
aggrecan
articular-cartilage
Cartilage
Cartilage repair
degeneration
double-blind trial
electromagnetic-fields
gene-expression
Gurkan I
Horton W E
Huckle J
in-vitro
Journal Article or Conference Abstract Publication
knee osteoarthritis
matrix production
Orthopedics
Osteoarthritis
Osteoarthritis and cartilage
placebo-controlled trials
Pleshko N
Ranganathan A
Rheumatology
Spencer R G
therapeutic ultrasound
tissue engineered cartilage
Todman M
Ultrasound
Yang X
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/rheumatology/kes363" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/rheumatology/kes363</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
998-1008
Issue
6
Volume
52
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Delphinidin Inhibits Il-1 Beta-induced Activation Of Nf-kappa B By Modulating The Phosphorylation Of Irak-1(ser376) In Human Articular Chondrocytes
Publisher
An entity responsible for making the resource available
Rheumatology
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-06
Subject
The topic of the resource
chondrocytes; COX-2; delphinidin; down-regulation; factor-alpha; gene-expression; human osteoarthritis chondrocytes; IL-1 beta; in-vitro; interleukin-1; kinase; nf-kappa-b; osteoarthritis; oxidative stress; PGE(2); rheumatoid-arthritis; Rheumatology; tumor-necrosis-factor
Creator
An entity primarily responsible for making the resource
Haseeb A; Chen D X; Haqqi T M
Description
An account of the resource
Objective. In OA, there is enhanced expression of pro-inflammatory cytokines such as IL-1 beta in the affected joint. Delphinidin, an anthocyanidin found in pigmented fruits and vegetables, has been shown to possess anti-inflammatory and antioxidant properties. In the present study we determined whether delphinidin would inhibit the IL-1 beta-induced activation of NF-kappa B in human chondrocytes and determined the mechanism of its action. Methods. PGE(2) levels and activation of NF-kappa B p65 in human OA chondrocytes were determined by ELISA-based assays. Protein expression of cyclo-oxygenase-2 (COX-2) and phosphorylation of kinases was determined by western immunoblotting. Expression level of mRNAs was determined by TaqMan assays. Results. Delphinidin inhibited IL-1 beta-induced expression of COX-2 and production of PGE(2) in human chondrocytes. Delphinidin also inhibited IL-1 beta-mediated phosphorylation of IL-1 receptor-associated kinase-1(Ser376), phosphorylation of IKK alpha/beta, expression of IKK beta, degradation of I kappa B alpha, and activation and nuclear translocation of NF-kappa B/p65. Phosphorylation of TGF-beta-activated kinase 1 was not observed but NF-kappa B-inducing kinase (NIK) was phosphorylated and phosphorylation of NIK was blocked by delphinidin in IL-1 beta-treated human chondrocytes. Conclusion. These data identify delphinidin as a novel inhibitor of IL-1 beta-induced production of cartilage-degrading molecule PGE(2) via inhibition of COX-2 expression and provide new insight into the mechanism of its action. Our results also identify inhibition of IRAK1(Ser376) phosphorylation by delphinidin in IL-1 beta-induced activation of NF-kappa B in human chondrocytes. Given the important role played by IL-1 beta-induced NF-kappa B activation, COX-2 expression and PGE(2) production in OA, our results may have important implications for the development of novel therapeutic strategies for the prevention/treatment of OA.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/rheumatology/kes363" target="_blank" rel="noreferrer noopener">10.1093/rheumatology/kes363</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2013
Chen D X
Chondrocytes
COX-2
delphinidin
Down-Regulation
factor-alpha
gene-expression
Haqqi T M
Haseeb A
human osteoarthritis chondrocytes
IL-1 beta
in-vitro
interleukin-1
Journal Article or Conference Abstract Publication
Kinase
nf-kappa-b
Osteoarthritis
Oxidative Stress
PGE(2)
rheumatoid-arthritis
Rheumatology
tumor-necrosis-factor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s10029-012-0941-2" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s10029-012-0941-2</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
677-687
Issue
6
Volume
16
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bone Marrow-derived Mesenchymal Stromal Cells And Platelet-rich Plasma On A Collagen Matrix To Improve Fascial Healing
Publisher
An entity responsible for making the resource available
Hernia
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-12
Subject
The topic of the resource
chronic wounds; closure; growth-factor; Hernia; in-vitro; incisional hernia; Mesenchymal; PRP (platelet-rich plasma); rat; repair; Stem; stem-cells; Stromal; Surgery; suture; tissue
Creator
An entity primarily responsible for making the resource
Heffner J J; Holmes J W; Ferrari J P; Krontiris-Litowitz J; Marie H; Fagan D L; Perko J C; Dorion H A
Description
An account of the resource
To demonstrate improved healing of a midline laparotomy after application of mesenchymal stromal cells and platelet-rich plasma on a collagen matrix and introduce a potential cellular-based therapy for the prevention of incisional hernia formation. Up to 10 % of laparotomies are complicated by postoperative incisional hernias. Despite continuous improvements in surgical technique and technology, hernia rates have remained constant. Cell-based therapies focused on augmentation of the body's natural healing properties could reduce hernia formation. Midline laparotomies were performed on 42 Lewis rats. Three groups were studied: (1) primary repair only, (2) primary repair with CollaTape (TM) (CoTa) overlay and platelet-rich plasma (PRP), and (3) primary repair with CoTa overlay and PRP and bone marrow-derived mesenchymal stromal cells (BM-MSCs). Abdominal wall fascia was recovered at 4 and 8 weeks in each group. Biomechanical testing and histological evaluation was performed. At 4 weeks, there was a twofold increase in tensile strength between groups 1 and 2 and a fourfold increase between groups 1 and 3 (p < 0.001). Group 3 had a 320 % increase in total energy absorption at 4 weeks compared to group 1 and a 142 % increase at 8 weeks (p < 0.001). Vascularization and collagen abundance were significantly increased in group 3 at both time points. The addition of BM-MSCs, PRP, and CoTa led to a marked improvement in abdominal wall strength and energy absorption. Histologic evaluation confirmed increased vascularity and collagen abundance consistent with the biomechanical findings. Application of this therapy may ultimately reduce incisional hernia formation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s10029-012-0941-2" target="_blank" rel="noreferrer noopener">10.1007/s10029-012-0941-2</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2012
chronic wounds
closure
Dorion H A
Fagan D L
Ferrari J P
growth-factor
Heffner J J
Hernia
Holmes J W
in-vitro
incisional hernia
Journal Article or Conference Abstract Publication
Krontiris-Litowitz J
Marie H
Mesenchymal
Perko J C
PRP (platelet-rich plasma)
rat
repair
Stem
stem-cells
Stromal
Surgery
suture
tissue
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1089/ten.2006.12.691" target="_blank" rel="noreferrer noopener">http://doi.org/10.1089/ten.2006.12.691</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
691-703
Issue
4
Volume
12
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Comparison Of Different Chondrocytes For Use In Tissue Engineering Of Cartilage Model Structures
Publisher
An entity responsible for making the resource available
Tissue Engineering
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-04
Subject
The topic of the resource
articular-cartilage; auricular cartilage; Cell Biology; construct; growth; in-vitro; regeneration; scaffold; shape; stem-cells; vivo
Creator
An entity primarily responsible for making the resource
Isogai N; Kusuhara H; Ikada Y; Ohtani H; Jacquet R; Hillyer J; Lowder E; Landis W J
Description
An account of the resource
This study compares bovine chondrocytes harvested from four different animal locations-nasoseptal, articular, costal, and auricular-for tissue-engineered cartilage modeling. While the work serves as a preliminary investigation for fabricating a human ear model, the results are important to tissue-engineered cartilage in general. Chondrocytes were cultured and examined to determine relative cell proliferation rates, type II collagen and aggrecan gene expression, and extracellular matrix production. Respective chondrocytes were then seeded onto biodegradable poly(L-lactide-epsilon-caprolactone) disc-shaped scaffolds. Cell-copolymer constructs were cultured and subsequently implanted in the subcutaneous space of athymic mice for up to 20 weeks. Neocartilage development in harvested constructs was assessed by molecular and histological means. Cell culture followed over periods of up to 4 weeks showed chondrocyte proliferation from the tissue sources varied, as did levels of type II collagen and aggrecan gene expression. For both genes, highest expression was found for costal chondrocytes, followed by nasoseptal, articular, and auricular cells. Retrieval of 20-week discs from mice revealed changes in construct dimensions with different chondrocytes. Greatest disc diameter was found for scaffolds seeded with auricular chondrocytes, followed by those with costal, nasoseptal, and articular cells. Greatest disc thickness was measured for scaffolds containing costal chondrocytes, followed by those with nasoseptal, auricular, and articular cells. Retrieved copolymer alone was smallest in diameter and thickness. Only auricular scaffolds developed elastic fibers after 20 weeks of implantation. Type II collagen and aggrecan were detected with differing expression levels on quantitative RT-PCR of discs implanted for 20 weeks. These data demonstrate that bovine chondrocytes obtained from different cartilaginous sites in an animal may elicit distinct responses during their respective development of a tissue-engineered neocartilage. Thus, each chondrocyte type establishes or maintains its particular developmental characteristics, and this observation is critical in the design and elaboration of any tissue-engineered cartilage model.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1089/ten.2006.12.691" target="_blank" rel="noreferrer noopener">10.1089/ten.2006.12.691</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2006
articular-cartilage
auricular cartilage
Cell Biology
construct
growth
Hillyer J
Ikada Y
in-vitro
Isogai N
Jacquet R
Journal Article or Conference Abstract Publication
Kusuhara H
Landis W J
Lowder E
Ohtani H
Regeneration
scaffold
shape
stem-cells
Tissue Engineering
vivo
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1023/a:1023936503105" target="_blank" rel="noreferrer noopener">http://doi.org/10.1023/a:1023936503105</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
147-154
Issue
3
Volume
39
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Experiments With Osteoblasts Cultured Under Varying Orientations With Respect To The Gravity Vector
Publisher
An entity responsible for making the resource available
Cytotechnology
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002
Subject
The topic of the resource
attachment; averaged gravity; Biotechnology & Applied Microbiology; bone; Cell Biology; cell-culture; clinostat; gene-expression; growth; growth rate; in-vitro; inversion; microgravity; osteoblasts; proliferation; space; substrate
Creator
An entity primarily responsible for making the resource
Kacena M A; Todd P; Gerstenfeld L C; Landis W J
Description
An account of the resource
Substrate attachment is crucial for normal growth and differentiation of many cell types. To better understand the role of gravity in osteoblast attachment and growth in vitro, 17-day-old embryonic chick calvarial osteoblasts were subjected to directional variations with respect to gravity. Osteoblasts, grown in MEM or DME supplemented with 10% FBS and attached to type I collagen-coated coverslips, were loaded into cylindrical containers completely filled with medium and oriented so that cells were either atop or beneath, or coverslips continuously rotated (similar to2 rpm) in a clinostat, thereby continuously changing their orientation with respect to gravity. Cells in these three conditions were collected daily for up to 6 days, and cell viability, two osteoblast functions, and proliferation were assessed. Data suggest the number and function of attached osteoblasts is unaltered by inversion or clino-rotation in initially confluent cultures. In sparsely plated cultures, however, osteoblast viability was significantly decreased (similar to50%) in inverted and rotated cultures during the first 3 days of sampling, but from days 4 - 6 no significant difference was found in viable cell number for the three conditions. Decreases in viable cell number within the first days of the experiments could result from death followed by detachment, detachment followed by death, differences in proliferation rate, or lag-phase duration. To help distinguish among these, BrdU labeling for 2 or 24 hr was used to assess cell proliferation rate. Log-phase growth rates were calculated and were unchanged among the three conditions tested. These results point to an increase in lag-phase duration in inverted and rotated cultures. In summary, changing the cell-substrate attachment direction with respect to gravity causes an immediate response in the form of diminished viable osteoblast number in sparse, early cultures, but the effect disappears after 3 - 4 days and does not occur in mature, confluent cultures.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1023/a:1023936503105" target="_blank" rel="noreferrer noopener">10.1023/a:1023936503105</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2002
attachment
averaged gravity
Biotechnology & Applied Microbiology
Bone
Cell Biology
cell-culture
clinostat
Cytotechnology
gene-expression
Gerstenfeld L C
growth
growth rate
in-vitro
inversion
Journal Article or Conference Abstract Publication
Kacena M A
Landis W J
microgravity
Osteoblasts
proliferation
space
substrate
Todd P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf02870949" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf02870949</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
28-34
Issue
1
Volume
15
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Experiments With Osteoblasts Cultured Under Hypergravity Conditions
Publisher
An entity responsible for making the resource available
Microgravity Science and Technology
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004
Subject
The topic of the resource
bone; cells; differentiation; Engineering; expression; human dermal fibroblasts; in-vitro; Mechanics; microgravity; rats; space-flight; system; Thermodynamics
Creator
An entity primarily responsible for making the resource
Kacena M A; Todd P; Gerstenfeld L C; Landis W J
Description
An account of the resource
To understand further the role of gravity in osteoblast attachment, osteoblasts were subjected to hypergravity conditions in vitro. Scanning electron microscopy of all confluent coverslips from FPA units show that the number of attached osteoblasts was similar among gravitational levels and growth durations (similar to90 cells/microscopic field). Specifically. confluent 1.0G control cultures contained an average of 91+/-8 cells/field, 3.3G samples had 88+/-8 cells/field, and 4.0G cultures averaged 90+/-7 cells/field. The sparsely plated cultures assessed by immunohistochemistry also had similar numbers of cells at each time point (1.0G was similar to 3.3 and 4.0G), but cell number changed from one time point to the next as those cells proliferated Immunohistochemistry of centrifuged samples showed an increase in number (up to 160% increase) and thickness (tip to 49% increase) of actin fibers, a decrease in intensity of fibro-nectin fluorescence (18-23% decrease) and an increase in number of vinculin bulbs (202-374% increase in number of vinculin bulbs/area). While hypergravity exposure did not alter the number of attached osteoblasts, it did result in altered actin, fibronectin, and vinculin elements, changing some aspects of osteoblast-substrate adhesion.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/bf02870949" target="_blank" rel="noreferrer noopener">10.1007/bf02870949</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2004
Bone
Cells
differentiation
Engineering
expression
Gerstenfeld L C
human dermal fibroblasts
in-vitro
Journal Article or Conference Abstract Publication
Kacena M A
Landis W J
mechanics
microgravity
Microgravity Science and Technology
Rats
space-flight
system
Thermodynamics
Todd P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
454-459
Issue
10
Volume
39
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Dublin Core
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Title
A name given to the resource
Osteoblasts Subjected To Spaceflight And Simulated Space Shuttle Launch Conditions
Publisher
An entity responsible for making the resource available
In Vitro Cellular & Developmental Biology-Animal
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-11
Subject
The topic of the resource
acceleration; bone cells; bone-formation; Cell Biology; cells; Developmental Biology; differentiation; flight; gene-expression; growing rats; in-vitro; messenger-rna expression; microgravity; vibration; weightlessness
Creator
An entity primarily responsible for making the resource
Kacena M A; Todd P; Landis W J
Description
An account of the resource
To understand further the effects of spaceflight on osteoblast-enriched cultures, normal chicken calvarial osteoblasts were flown aboard shuttle flight STS-77, and the total number of attached cells was determined. Spaceflight and control cultures were chemically fixed 3 h and 3 d after launch. These fixed cultures were processed for scanning electron microscopy (SEM). The SEM analysis showed that with just 3 d of exposure to spaceflight, coverslip cultures contained 300 +/- 100 cells/mm(2), whereas 1G control samples contained a confluent monolayer of cells (2400 +/- 200 cells/mm(2)). Although the cultures flown in space experienced a drastic decline in cell number in just 3 d, without further experimentation it was impossible to determine whether the decline was a result of microgravity, the harsh launch environment, or some combination of these factors. Therefore, this research attempted to address the effect of launch by subjecting osteoblasts to conditions simulating shuttle launch accelerations, noise, and vibrations. No differences, compared with controls, were seen in the number of total or viable cells after exposure to these various launch conditions. Taken together, these data indicate that the magnitude of gravitational loading (3G maximum) and vibration (7.83G rms maximum) resulting from launch does not adversely affect osteoblasts in terms of total or viable cell number immediately, but launch conditions, or the microgravity environment itself, may start a cascade of events that over several d contributes to cell loss.
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2003
acceleration
bone cells
bone-formation
Cell Biology
Cells
Developmental Biology
differentiation
Flight
gene-expression
growing rats
In Vitro Cellular & Developmental Biology-Animal
in-vitro
Journal Article or Conference Abstract Publication
Kacena M A
Landis W J
messenger-rna expression
microgravity
Todd P
vibration
Weightlessness
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/pmic.200500447" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/pmic.200500447</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1250-1260
Issue
4
Volume
6
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Increased Oxidation And Degradation Of Cytosolic Proteins In Alcohol-exposed Mouse Liver And Hepatoma Cells
Publisher
An entity responsible for making the resource available
Proteomics
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-02
Subject
The topic of the resource
alcoholism; apoptosis; Biochemistry & Molecular Biology; CYP2E1; cytochrome p-4502e1; Degradation; disease; ethanol; in-vitro; induced; injury; nitric-oxide; oxidative stress; oxidized proteins; peroxiredoxin; protein; protein oxidation; rat-liver; stress
Creator
An entity primarily responsible for making the resource
Kim B J; Hood B L; Aragon R A; Hardwick Jr; Conrads T R; Veenstra T D; Song B J
Description
An account of the resource
We recently developed a sensitive method using biotin-N-maleimide (biotin-NM) as a probe to positively identify oxidized mitochondrial proteins. In this study, biotin-NM was used to identify oxidized cytosolic proteins in alcohol-fed mouse livers. Alcohol treatment for 6 wk elevated the levels of CYP2E1 and nitrotyrosine, a marker of oxidative stress. Markedly increased levels of oxidized proteins were detected in alcohol-fed mouse livers compared to pair-fed controls. The biotin-NM-labeled oxidized proteins from alcohol-exposed mouse livers were subsequently purified with streptavidin-agarose and resolved on 2-DE. More than 90 silver-stained protein spots that displayed differential intensities on 2-D gels were identified by MS. Peptide sequence analysis revealed that many enzymes or proteins involved in stress response, chaperone activity, intermediary metabolism, and antioxidant defense systems such as peroxiredoxin were oxidized after alcohol treatment. Smaller fragments of many proteins were repeatedly detected only in alcohol-fed mice, indicating that many oxidized proteins after alcohol exposure were degraded. Immunoblot results showed that the level of oxidized peroxiredoxin (inactivated) was markedly increased in the alcohol-exposed mouse livers and ethanol-sensitive hepatoma cells compared to the corresponding controls. Our results may explain the underlying mechanism for cellular dysfunction and increased susceptibility to other toxic agents following alcohol-mediated oxidative stress.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/pmic.200500447" target="_blank" rel="noreferrer noopener">10.1002/pmic.200500447</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2006
alcoholism
Apoptosis
Aragon R A
Biochemistry & Molecular Biology
Conrads T R
CYP2E1
cytochrome p-4502e1
degradation
Disease
ETHANOL
Hardwick Jr
Hood B L
in-vitro
Induced
Injury
Journal Article or Conference Abstract Publication
Kim B J
nitric-oxide
Oxidative Stress
oxidized proteins
peroxiredoxin
Protein
Protein oxidation
proteomics
rat-liver
Song B J
Stress
Veenstra T D
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1111/j.1524-475X.2008.00451.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1111/j.1524-475X.2008.00451.x</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
136-146
Issue
1
Volume
17
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Tissue Engineering A Model For The Human Ear: Assessment Of Size, Shape, Morphology, And Gene Expression Following Seeding Of Different Chondrocytes
Publisher
An entity responsible for making the resource available
Wound Repair and Regeneration
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-01
Subject
The topic of the resource
auricular reconstruction; bone sialoprotein; cartilage tissue; Cell Biology; cells; costal cartilage; culture; Dermatology; growth-plate; in-vitro; regeneration; Research & Experimental Medicine; Surgery; transplantation
Creator
An entity primarily responsible for making the resource
Kusuhara H; Isogai N; Enjo M; Otani H; Ikada Y; Jacquet R; Lowder E; Landis W J
Description
An account of the resource
This study examines the tissue engineering of a human ear model through use of bovine chondrocytes isolated from four different cartilaginous sites (nasoseptal, articular, costal, and auricular) and seeded onto biodegradable poly(l-lactic acid) and poly(l-lactide-epsilon-caprolactone) (50 : 50) polymer ear-shaped scaffolds. After implantation in athymic mice for up to 40 weeks, cell/scaffold constructs were harvested and analyzed in terms of size, shape, histology, and gene expression. Gross morphology revealed that all the tissue-engineered cartilages retained the initial human auricular shape through 40 weeks of implantation. Scaffolds alone lost significant size and shape over the same period. Quantitative reverse transcription-polymerase chain reaction demonstrated that the engineered chondrocyte/scaffolds yielded unique expression patterns for type II collagen, aggrecan, and bone sialoprotein mRNA. Histological analysis showed type II collagen and proteoglycan to be the predominant extracellular matrix components of the various constructs sampled at different implantation times. Elastin was also present but it was found only in constructs seeded with auricular chondrocytes. By 40 weeks of implantation, tissue-engineered cartilage of costal origin became calcified, marked by a notably high relative gene expression level of bone sialoprotein and the presence of rigid, nodular protrusions formed by mineralizing rudimentary cartilaginous growth plates. The collective data suggest that nasoseptal, articular, and auricular cartilages represent harvest sites suitable for development of tissue-engineered human ear models with retention over time of three-dimensional construct architecture, gene expression, and extracellular matrix composition comparable to normal, nonmineralizing cartilages. Calcification of constructs of costal chondrocyte origin clearly shows that chondrocytes from different tissue sources are not identical and retain distinct characteristics and that these specific cells are inappropriate for use in engineering a flexible ear model.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1111/j.1524-475X.2008.00451.x" target="_blank" rel="noreferrer noopener">10.1111/j.1524-475X.2008.00451.x</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2009
auricular reconstruction
bone sialoprotein
cartilage tissue
Cell Biology
Cells
costal cartilage
Culture
Dermatology
Enjo M
growth-plate
Ikada Y
in-vitro
Isogai N
Jacquet R
Journal Article or Conference Abstract Publication
Kusuhara H
Landis W J
Lowder E
Otani H
Regeneration
Research & Experimental Medicine
Surgery
Transplantation
Wound Repair and Regeneration
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1359/jbmr.2000.15.6.1099" target="_blank" rel="noreferrer noopener">http://doi.org/10.1359/jbmr.2000.15.6.1099</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1099-1112
Issue
6
Volume
15
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Title
A name given to the resource
Spaceflight Effects On Cultured Embryonic Chick Bone Cells
Publisher
An entity responsible for making the resource available
Journal of Bone and Mineral Research
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-06
Subject
The topic of the resource
biomechanics; bone adaptation; collagen; differentiation; Endocrinology & Metabolism; in-vitro; mechanical strain; messenger-rna expression; microgravity; osteoblast; osteoblasts; osteopontin; restriction; signal-transduction; space-flight
Creator
An entity primarily responsible for making the resource
Landis W J; Hodgens K J; Block D; Toma C D; Gerstenfeld L C
Description
An account of the resource
A model calcifying system of primary osteoblast cell cultures derived from normal embryonic chicken calvaria has been flown aboard the shuttle, Endeavour, during the National Aeronautics and Space Administration (NASA) mission STS-59 (April 9-20, 1994) to characterize unloading and other spaceflight effects on the bone cells. Aliquots of cells (similar to 7 x 10(6)) grown in Dulbecco's modified Eagle's medium (DMEM) + 10% fetal bovine serum (FBS) were mixed with microcarrier beads, inoculated into cartridge culture units of artificial hollow fiber capillaries, and carried on the shuttle. To promote cell differentiation, cartridge media were supplemented with 12.5 mu g/ml ascorbate and 10 mM P-glycerophosphate for varying time periods before and during Right. Four cartridges contained cells from 17-day-old embryos grown for 5 days in the presence of ascorbate prior to launch (defined as flight cells committed to the osteoblastic lineage) and four cartridges supported cells from 14-day-old embryos grown for 10 days with ascorbate before launch (uncommitted flight cells). Eight cartridges prepared in the same manner were maintained under normal gravity throughout the Right (control cells) and four additional identical cartridges under normal gravity were terminated on the day of launch (basal cells). From shuttle launch to landing, all cartridges were contained in closed hardware units maintaining 5% CO2, 37 degrees C, and media delivery at a rate of similar to 1.5 ml/6 h. During day 3 and day 5 of flight, duplicate aliquots of conditioned media and accumulated cell products were collected in both the Right and the control hardware units. At the mission end, comparisons among Right, basal, and control samples were made in cell metabolism gene expression for type I collagen and osteocalcin, and ultrastructure. Both committed and uncommitted flight cells were metabolically active, as measured by glucose uptake and lactate production, at approximately the same statistical levels as control counterparts. Flight cells elaborated a less extensive extracellular matrix, evidenced by a reduced collagen gene expression and collagen protein appearance compared with controls. Osteocalcin was expressed by all cells, a result indicating progressive differentiation of both flight and control osteoblasts, but its message levels also were reduced in flight cells compared with ground samples. This finding suggested that osteoblasts subjected to flight followed a slower progression toward a differentiated function. The summary of data indicates that spaceflight, including microgravity exposure, demonstrably affects bone cells by down-regulating type I collagen and osteocalcin gene expression and thereby inhibiting expression of the osteogenic phenotype notably by committed osteoblasts. The information is important for insight into the response of bone cells to changes of gravity and of force in general.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1359/jbmr.2000.15.6.1099" target="_blank" rel="noreferrer noopener">10.1359/jbmr.2000.15.6.1099</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2000
biomechanics
Block D
Bone adaptation
Collagen
differentiation
Endocrinology & Metabolism
Gerstenfeld L C
Hodgens K J
in-vitro
Journal Article or Conference Abstract Publication
Journal of Bone and Mineral Research
Landis W J
mechanical strain
messenger-rna expression
microgravity
OSTEOBLAST
Osteoblasts
Osteopontin
restriction
signal-transduction
space-flight
Toma C D
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0304-3940(91)90494-e" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0304-3940(91)90494-e</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1-4
Issue
1
Volume
134
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Prolactin Stimulates Dopamine Release From The Rat Corpus Striatum In The Absence Of Extracellular Calcium
Publisher
An entity responsible for making the resource available
Neuroscience Letters
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991-12
Subject
The topic of the resource
amphetamine; behavior; binding-sites; brain; calcium; cerebrospinal-fluid; corpus striatum; dopamine; hypothalamus; in-vitro; Neurosciences & Neurology; prolactin; rat
Creator
An entity primarily responsible for making the resource
Laping N J; Dluzen D E; Ramirez V D
Description
An account of the resource
Prolactin (PRL) increased basal dopamine (DA) release and attenuated amphetamine (AMPH)-stimulated DA release in vitro from rat corpus striatum in a concentration-dependent manner with 10(-5) M PRL being the most effective. The effects of PRL on DA release were enhanced in the absence of extracellular calcium. PRL at 10(-5) M did not alter the DA post-superfusion content of the striatal tissue. These results indicate that the stimulatory effect of PRL on basal DA release does not require extra-cellular calcium and the inhibitory effect on AMPH-stimulated DA release is not due to depletion of DA stores.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0304-3940(91)90494-e" target="_blank" rel="noreferrer noopener">10.1016/0304-3940(91)90494-e</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1991
amphetamine
Behavior
binding-sites
Brain
calcium
cerebrospinal-fluid
corpus striatum
Dluzen D E
Dopamine
Hypothalamus
in-vitro
Journal Article or Conference Abstract Publication
Laping N J
Neuroscience letters
Neurosciences & Neurology
prolactin
Ramirez V D
rat
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0014-2999(92)90390-p" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0014-2999(92)90390-p</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
337-341
Issue
3
Volume
211
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The Actions Of 3-aminopropanephosphinic Acid At Gaba(b) Receptors In Rat Hippocampus
Publisher
An entity responsible for making the resource available
European Journal of Pharmacology
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-02
Subject
The topic of the resource
2-hydroxy-saclofen; b receptors; baclofen; brain slices; ca3 pyramidal cells; excitatory transmission; gaba(b) receptors (postsynaptic); gaba(b) receptors (presynaptic); inhibitory transmission; in-vitro; neurons; patch; Pharmacology & Pharmacy; potent; slice recording; synapses; transmission
Creator
An entity primarily responsible for making the resource
Lovinger D M; Harrison N L; Lambert N A
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0014-2999(92)90390-p" target="_blank" rel="noreferrer noopener">10.1016/0014-2999(92)90390-p</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1992
2-hydroxy-saclofen
b receptors
baclofen
brain slices
ca3 pyramidal cells
European journal of pharmacology
excitatory transmission
gaba(b) receptors (postsynaptic)
gaba(b) receptors (presynaptic)
Harrison N L
in-vitro
inhibitory transmission
Lambert N A
Lovinger D M
Neurons
patch
Pharmacology & Pharmacy
potent
slice recording
synapses
Transmission
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1111/j.1471-4159.2010.06948.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1111/j.1471-4159.2010.06948.x</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
515-525
Issue
2
Volume
115
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Chronic Exposure To Nicotine And Saquinavir Decreases Endothelial Notch-4 Expression And Disrupts Blood-brain Barrier Integrity
Publisher
An entity responsible for making the resource available
Journal of Neurochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-10
Subject
The topic of the resource
acetylcholine-receptors; Biochemistry & Molecular Biology; choline transporter; cigarette-smoke condensate; electron-spin-resonance; endothelial cell toxicity; gene-expression; in-vitro; inhibitors; Neurosciences & Neurology; nicotine; Notch-4; osmotic pumps; parkinsons-disease; perfusion technique; protease; protease inhibitors; proteins; ritonaivir; ROS; saquinqavir; tight junction
Creator
An entity primarily responsible for making the resource
Manda V K; Mittapalli R K; Geldenhuys W J; Lockman P R
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1111/j.1471-4159.2010.06948.x" target="_blank" rel="noreferrer noopener">10.1111/j.1471-4159.2010.06948.x</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2010
acetylcholine-receptors
Biochemistry & Molecular Biology
Choline transporter
cigarette-smoke condensate
electron-spin-resonance
endothelial cell toxicity
Geldenhuys W J
gene-expression
in-vitro
inhibitors
Journal of neurochemistry
Lockman P R
Manda V K
Mittapalli R K
Neurosciences & Neurology
Nicotine
Notch-4
osmotic pumps
parkinsons-disease
perfusion technique
protease
protease inhibitors
Proteins
ritonaivir
ROS
saquinqavir
tight junction
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/1120009x.2002.11782351" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/1120009x.2002.11782351</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
42-56
Volume
14
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Clinical and public health implications of macrolide-resistant Streptococcus pneumoniae
Publisher
An entity responsible for making the resource available
Journal of Chemotherapy
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-07
Subject
The topic of the resource
Infectious Diseases; Pathology; united-states; Pharmacology & Pharmacy; in-vitro; Oncology; community-acquired pneumonia; macrolide resistance; streptococci; respiratory; pneumoniae; haemophilus-influenzae; moraxella-catarrhalis; tract infections; macrolides; otitis-media; group-a; erythromycin resistance; decreased susceptibility; initial antimicrobial therapy; pneumococcal resistance; S.; treatment failure
Creator
An entity primarily responsible for making the resource
Moellering R C; Consensus Working Grp
Description
An account of the resource
Macrolide resistance among Streptococcus pneumoniae is a growing global concern, although its specific impact on public health is not currently well defined. A Consensus Working Group was convened in March 2001 to address whether credible, scientific data substantiate macrolide resistance in S. pneumoniae as: (i) producing significant morbidity; (ii) creating attendant health and economic burdens; (iii) constituting a public health threat; and (iv) warranting intervention, including development of new antibiotics with efficacy against these strains. Despite the limitations of available clinical data, concern about the possibility of treatment failure with macrolides is being expressed in clinical practice and in formal treatment guidelines, threatening the important role of these agents in the treatment of respiratory tract infections. Further studies are required to monitor and control macrolide resistance and evaluate settings in which macrolide treatment failures are occurring, and new therapeutic interventions are needed.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/1120009x.2002.11782351" target="_blank" rel="noreferrer noopener">10.1080/1120009x.2002.11782351</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2002
Community-acquired pneumonia
Consensus Working Grp
decreased susceptibility
erythromycin resistance
group-a
haemophilus-influenzae
in-vitro
Infectious Diseases
initial antimicrobial therapy
Journal Article or Conference Abstract Publication
Journal of Chemotherapy
macrolide resistance
macrolides
Moellering R C
moraxella-catarrhalis
oncology
otitis-media
Pathology
Pharmacology & Pharmacy
pneumococcal resistance
pneumoniae
respiratory
S.
streptococci
tract infections
Treatment Failure
united-states
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/adem.201500282" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/adem.201500282</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
518-531
Issue
4
Volume
18
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A Novel Hybrid-Structured Titanium Surface Promotes Adhesion of Human Dermal Fibroblasts and Osteogenesis of Human Mesenchymal Stem Cells while Reducing S-epidermidis Biofilm Accumulation
Publisher
An entity responsible for making the resource available
Advanced Engineering Materials
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-04
Subject
The topic of the resource
differentiation; energy; hydrophilicity; implants; in-vitro; Materials Science; nanotopography; osseointegration; osteoactivin; osteoblast lineage cells; responses
Creator
An entity primarily responsible for making the resource
Park B W; Krieger J; Sondag G R; Moussa F M; Rankenberg J; Safadi F F; Gatsonis N A; McGimpsey W G; Lambert C R; Malcuit C
Description
An account of the resource
We provide a comparative analysis of protein adsorption, primary human cell behavior, and biofilm formation on modified titanium substrates of either micro-, nano-, or hybrid micro/nano-scale feature sizes. While studies revealed that nano-scale structures initially decreased the attachment and spreading of both human fibroblasts (hDFs) and mesenchymal stem cells (hMSCs), hMSC differentiation studies revealed that hybrid structures promoted the highest levels of osteogenic gene expression and attenuated biofilm formation by Staphylococcus epidermidis. Taken together, this novel approach of generating a hybrid topographical feature results in a potential implant material capable of enhanced dermal cell adhesion and osteogenic differentiation while limiting biofilm accumulation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/adem.201500282" target="_blank" rel="noreferrer noopener">10.1002/adem.201500282</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2016
Advanced Engineering Materials
differentiation
energy
Gatsonis N A
hydrophilicity
Implants
in-vitro
Journal Article
Krieger J
Lambert C R
Malcuit C
Materials Science
McGimpsey W G
Moussa F M
nanotopography
osseointegration
OSTEOACTIVIN
osteoblast lineage cells
Park B W
Rankenberg J
responses
Safadi F F
Sondag G R
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1111/j.1525-142X.2005.05048.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1111/j.1525-142X.2005.05048.x</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
440-457
Issue
5
Volume
7
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Dublin Core
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Title
A name given to the resource
Morphoregulation of teeth: modulating the number, size, shape and differentiation by tuning Bmp activity
Publisher
An entity responsible for making the resource available
Evolution & Development
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-09
Subject
The topic of the resource
ameloblast; Bone morphogenetic protein-2; dentition; Developmental Biology; differentiation; early tooth development; enamel knot; Evolutionary Biology; Genetics & Heredity; homeobox genes; in-vitro; missense mutation; molar teeth; murine; signaling pathways
Creator
An entity primarily responsible for making the resource
Plikus M V; Zeichner-David M; Mayer J A; Reyna J; Bringas P; Thewissen J G M; Snead M L; Chai Y; Chuong C M
Description
An account of the resource
During development and evolution, the morphology of ectodermal organs can be modulated so that an organism can adapt to different environments. We have proposed that morphoregulation can be achieved by simply tilting the balance of molecular activity. We test the principles by analyzing the effects of partial downregulation of Bmp signaling in oral and dental epithelia of the keratin 14-Noggin transgenic mouse. We observed a wide spectrum of tooth phenotypes. The dental formula changed from 1.0.0.3/1.0.0.3 to 1.0.0.2(1)/1.0.0.0. All mandibular and M3 maxillary molars were selectively lost because of the developmental block at the early bud stage. First and second maxillary molars were reduced in size, exhibited altered crown patterns, and failed to form multiple roots. In these mice, incisors were not transformed into molars. Histogenesis and differentiation of ameloblasts and odontoblasts in molars and incisors were abnormal. Lack of enamel caused misocclusion of incisors, leading to deformation and enlargement in size. Therefore, subtle differences in the level, distribution, and timing of signaling molecules can have major morphoregulatory consequences. Modulation of Bmp signaling exemplifies morphoregulation hypothesis: simple alteration of key signaling pathways can be used to transform a prototypical conical-shaped tooth into one with complex morphology. The involvement of related pathways and the implication of morphoregulation in tooth evolution are discussed.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1111/j.1525-142X.2005.05048.x" target="_blank" rel="noreferrer noopener">10.1111/j.1525-142X.2005.05048.x</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2005
ameloblast
Bone morphogenetic protein-2
Bringas P
Chai Y
Chuong C M
Dentition
Developmental Biology
differentiation
early tooth development
enamel knot
Evolution & Development
Evolutionary Biology
Genetics & Heredity
homeobox genes
in-vitro
Journal Article
Mayer J A
missense mutation
molar teeth
murine
Plikus M V
Reyna J
signaling pathways
Snead M L
Thewissen J G M
Zeichner-David M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/03008200701692685" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/03008200701692685</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
316-323
Issue
6
Volume
48
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Analysis of human osteoarthritic connective tissue by laser capture microdissection and QRT-PCR
Publisher
An entity responsible for making the resource available
Connective Tissue Research
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007
Subject
The topic of the resource
adult articular-cartilage; aggrecan gene expression; arthroplasty; cartilage; Cell Biology; chondrocytes; disease; gene-expression; human osteoarthritis; in-vitro; knee; laser capture; microdissection; molecular analysis; Orthopedics; QRT-PCR; quality-of-life; replacement surgery; skeletal tissues; type II collagen
Creator
An entity primarily responsible for making the resource
Scharschmidt T; Jacquet R; Laskovski J; Lowder E; Weiner S; Landis W J
Description
An account of the resource
Gene expression levels for type II collagen and aggrecan have been determined as potential measures and disease markers of human osteoarthritis in patients undergoing total knee arthroplasty. In this regard, specimens of affected articular cartilage obtained intraoperatively at the time of surgery were placed in RNAlater (TM) to maintain RNA integrity and subsequently frozen-sectioned. Individual or small numbers of chondrocytes were isolated by laser capture microdissection and their total RNA was extracted and analyzed by quantitative reverse transcription-polymerase chain reaction. Results indicate that type II collagen and aggrecan mRNA expression from specific cells in osteoarthritic tissues are detectable and reproducible using these approaches. Our work is the first to demonstrate successful isolation of RNA limited to chondrocytes comprising small quantities of human osteoarthritic material. The study presents a new avenue by which the disease and its progression may be critically assayed.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/03008200701692685" target="_blank" rel="noreferrer noopener">10.1080/03008200701692685</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2007
adult articular-cartilage
aggrecan gene expression
Arthroplasty
Cartilage
Cell Biology
Chondrocytes
Connective tissue research
Disease
gene-expression
human osteoarthritis
in-vitro
Jacquet R
Journal Article
Knee
Landis W J
laser capture
Laskovski J
Lowder E
Microdissection
molecular analysis
Orthopedics
QRT-PCR
quality-of-life
replacement surgery
Scharschmidt T
skeletal tissues
type II collagen
Weiner S
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1208/s12249-014-0097-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1208/s12249-014-0097-8</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
741-752
Issue
3
Volume
15
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Title
A name given to the resource
Light-Activatable Gold Nanoshells for Drug Delivery Applications
Publisher
An entity responsible for making the resource available
Aaps Pharmscitech
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-06
Subject
The topic of the resource
cancer nanotheranostics; drug delivery; gold nanoshells; growth-factor receptor; in-vitro; nanocarrier; nanoparticles; nf-kappa-b; Pharmacology & Pharmacy; photothermal-chemotherapy; release; surface-plasmon resonance; targeted delivery; theranostics; thermal therapy; triggered; triggered release
Creator
An entity primarily responsible for making the resource
Singhana B; Slattery P; Chen A; Wallace M; Melancon M P
Description
An account of the resource
Gold nanoshells (AuNSs) are currently being investigated as nanocarriers for drug delivery systems and have both diagnostic and therapeutic applications, including photothermal ablation, hyperthermia, drug delivery, and diagnostic imaging, particularly in oncology. AuNSs are valuable for their localized surface plasmon resonance, biocompatibility, low immunogenicity, and facile functionalization. AuNSs used for drug delivery can be spatially and temporally triggered to release controlled quantities of drugs inside the target cells when illuminated with a near-infrared (NIR) laser. Recently, many research groups have demonstrated that these AuNS complexes are able to deliver antitumor drugs (e.g., doxorubicin, paclitaxel, small interfering RNA, and single-stranded DNA) into cancer cells, which enhances the efficacy of treatment. AuNSs can also be functionalized with active targeting ligands such as antibodies, aptamers, and peptides to increase the particles' specific binding to the desired targets. This article reviews the current research on NIR light-activatable AuNSs used as nanocarriers for drug delivery systems and cancer theranostics.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1208/s12249-014-0097-8" target="_blank" rel="noreferrer noopener">10.1208/s12249-014-0097-8</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2014
AAPS PharmSciTech
cancer nanotheranostics
Chen A
Drug delivery
gold nanoshells
growth-factor receptor
in-vitro
Journal Article
Melancon M P
nanocarrier
Nanoparticles
nf-kappa-b
Pharmacology & Pharmacy
photothermal-chemotherapy
release
Singhana B
Slattery P
surface-plasmon resonance
targeted delivery
Theranostics
thermal therapy
triggered
triggered release
Wallace M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/1097-4644(20010601)81:3%3C535::aid-jcb1067%3E3.0.co;2-6" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/1097-4644(20010601)81:3%3C535::aid-jcb1067%3E3.0.co;2-6</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
535-546
Issue
3
Volume
81
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Baculovirus-expressed vitamin D-binding protein-macrophage activating factor (DBP-maf) activates osteoclasts and binding of 25-hydroxyvitamin D-3 does not influence this activity
Publisher
An entity responsible for making the resource available
Journal of Cellular Biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001
Subject
The topic of the resource
activating factor (DBP-maf); albumin; alpha-fetoprotein; Biochemistry & Molecular Biology; bone resorption; bone resorption; Cell Biology; cells; family; gc-globulin; group-specific component; identification; in-vitro; member; osteoclast activation; recombinant DBP-maf; vitamin D-binding protein (DBP); vitamin D-binding protein macrophage
Creator
An entity primarily responsible for making the resource
Swamy N; Ghosh S; Schneider G B; Ray R
Description
An account of the resource
Vitamin D-binding protein (DBP) is a multi-functional serum protein that is converted to vitamin D-binding protein-macrophage activating factor (DBP-maf) by post-translational modification. DBP-maf is a new cytokine that mediates bone resorption by activating osteoclasts, which are responsible for resorption of bone. Defective osteoclast activation leads to disorders like osteopetrosis, characterized by excessive accumulation of bone mass. Previous studies demonstrated that two nonallelic mutations in the rat with osteopetrosis have independent defects in the cascade involved in the conversion of DBP to DBP-maf. The skeletal defects associated with osteopetrosis are corrected in these mutants with in vivo DBP-maf treatment. This study evaluates the effects of various forms of DBP-maf (native, recombinant, and 25-hydroxyvitamin D-3 bound) on osteoclast function in vitro in order to determine some of the structural requirements of this protein that relate to bone resorbing activities. Osteoclast activity was determined by evaluating pit formation using osteoclasts, isolated from the long bones of newborn rats, incubated on calcium phosphate coated, thin film, Ostologic MultiTest Slides. Incubation of osteoclasts with ex vivo generated native DBP-maf resulted in a dose dependent, statistically significant, activation of the osteoclasts. The activation was similar whether or not the vitamin D binding site of the DBP-maf was occupied. The level of activity in response to DBP-maf was greater than that elicited by optimal doses of other known stimulators (PTH and 1,25(OH)(2)D-3) of osteoclast function. Furthermore, another potent macrophage activating factor, interferon-gamma, had no effect on osteoclast activity. The activated form of a full length recombinant DBP, expressed in E. coli showed no activity in the in vitro assay. Contrary to this finding, baculovirus-expressed recombinant DBP-maf demonstrated significant osteoclast activating activity. The normal conversion of DBP to DBP-maf requires the selective removal of galactose and sialic acid from the third domain of the protein. Hence, the differential effects of the two recombinant forms of DBP-maf is most likely related to glycosylation; E. coli expressed recombinant DBP is non-glycosylated, whereas the baculovirus expressed form is glycosylated. These data support the essential role of glycosylation for the osteoclast activating property of DBP-mai. J. Cell. Biochem. 81:535-546, 2001. (C) 2001 Wiley-Liss, Inc.
Identifier
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<a href="http://doi.org/10.1002/1097-4644(20010601)81:3%3C535::aid-jcb1067%3E3.0.co;2-6" target="_blank" rel="noreferrer noopener">10.1002/1097-4644(20010601)81:3%3C535::aid-jcb1067%3E3.0.co;2-6</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2001
activating factor (DBP-maf)
albumin
Alpha-fetoprotein
Biochemistry & Molecular Biology
Bone resorption
Cell Biology
Cells
Family
gc-globulin
Ghosh S
group-specific component
identification
in-vitro
Journal Article
Journal of cellular biochemistry
member
osteoclast activation
Ray R
recombinant DBP-maf
Schneider G B
Swamy N
vitamin D-binding protein (DBP)
vitamin D-binding protein macrophage
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1021/la500905z" target="_blank" rel="noreferrer noopener">http://doi.org/10.1021/la500905z</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
5202-5208
Issue
18
Volume
30
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Dextran-Peptide Hybrid for Efficient Gene Delivery
Publisher
An entity responsible for making the resource available
Langmuir
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-05
Subject
The topic of the resource
cationic lipids; cells; Chemistry; cytotoxicity; drug delivery; in-vitro; Materials Science; nanoparticles; nonviral vector; plasmid dna; polyethylenimine; therapy
Creator
An entity primarily responsible for making the resource
Tang Q; Cao B; Lei X; Sun B B; Zhang Y Q; Cheng G
Description
An account of the resource
Gene therapy has drawn significant interest in the past two decades since it provides a promising strategy to treat both genetic disorders and acquired diseases. However, the transfer of gene therapy to clinical applications is troubled with many difficulties, since many current systems are of toxicity, low transfection efficiency and low biodegradability. To address these challenges, we developed a dextran-peptide hybrid system as a safe and efficient vector for gene therapy and investigated the structure-function-cytotoxicity relationship of this dextran-peptide hybrid system. Dextrans (Dex10, Dex20, and Dex70) with different molecular weights (10, 20 and 70 kDa) were conjugated with a cationic peptide, R5H5, at various degrees of substitution. Gene expression and cytotoxicity mediated by this delivery system were evaluated against SKOV-3 human ovarian carcinoma cells and compared to 25 kDa branched poly(ethylenimine) (PEI). The results showed that Dex10-R5H5 and Dex20-R5H5 hybrids derived from low molecular weight dextrans induced higher gene expression and lower cytotoxicity than Dex70-R5H5 hybrid from higher molecular weight dextran. The best performance on gene expression was achieved by Dex10-R5H5 at 40% substitution of R5H5, which induced greater gene expression than PEI at a low N/P ratio of S. Dex10-R5H5/DNA complexes at 40% substitution of R5H5 also showed much higher cell viability (93%) than PEI/DNA (66%) at the same N/P ratio. These results indicate that the Dex-R5H5 hybrid with the low molecular weight of dextran and the high degree of substitution of R5H5 is a very promising material for safe and efficient gene therapy.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1021/la500905z" target="_blank" rel="noreferrer noopener">10.1021/la500905z</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2014
Cao B
Cationic lipids
Cells
Chemistry
Cheng G
cytotoxicity
Drug delivery
in-vitro
Journal Article
Langmuir
Lei X
Materials Science
Nanoparticles
nonviral vector
plasmid dna
polyethylenimine
Sun B B
Tang Q
therapy
Zhang Y Q
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1039/c4ra12878h" target="_blank" rel="noreferrer noopener">http://doi.org/10.1039/c4ra12878h</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
28019-28022
Issue
35
Volume
5
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A naturally derived dextran-peptide vector for microRNA antagomir delivery
Publisher
An entity responsible for making the resource available
Rsc Advances
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015
Subject
The topic of the resource
cells; Chemistry; gene delivery; human cancers; in-vitro; mechanism; RNA interference; therapy; vivo
Creator
An entity primarily responsible for making the resource
Tang Q; Lei X; Cao B; Sun B B; Zhang Y Q; Cheng G
Description
An account of the resource
Single stranded microRNAs and their antagomirs are unstable and polyanionic, which impedes efficient cellular uptake and reduces half-life. Therefore, effective delivery systems with low toxicity for microRNAs are urgently needed for the success of microRNA-based therapy. Here, a dextran-peptide hybrid, Dex10-R5H5(40%), was developed as a carrier to deliver microRNAs. Dex10-R5H5(40%) loaded with antagomir-149 could reduce the level of endogenous microRNA-149 by 76% and it is more effective than the commercially available transfection reagent, RNAiMAX, which leads to 67% reduction. Additionally, Dex10-R5H5(40%) exhibited no cytotoxicity to HepG2 cells. These results indicate that the dextran-peptide hybrid may be a promising delivery system for the safe and efficient microRNA-based therapy.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1039/c4ra12878h" target="_blank" rel="noreferrer noopener">10.1039/c4ra12878h</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2015
Cao B
Cells
Chemistry
Cheng G
gene delivery
human cancers
in-vitro
Journal Article
Lei X
mechanism
RNA Interference
Rsc Advances
Sun B B
Tang Q
therapy
vivo
Zhang Y Q
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.4315/0362-028x.jfp-12-532" target="_blank" rel="noreferrer noopener">http://doi.org/10.4315/0362-028x.jfp-12-532</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1210-1217
Issue
7
Volume
76
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Title
A name given to the resource
Efficacy of Commonly Used Disinfectants for Inactivation of Human Noroviruses and Their Surrogates
Publisher
An entity responsible for making the resource available
Journal of Food Protection
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-07
Subject
The topic of the resource
Biotechnology & Applied Microbiology; enteric viruses; feline calicivirus; Food Science & Technology; gii.4 norovirus; hand sanitizers; in-vitro; murine norovirus; norwalk-like viruses; stainless-steel; surfaces; virucidal activity
Creator
An entity primarily responsible for making the resource
Tung G; Macinga D; Arbogast J; Jaykus L A
Description
An account of the resource
Human noroviruses (HuNoVs) are the most common cause of acute viral gastroenteritis worldwide and are a leading cause of foodborne disease. Their environmental persistence and purported resistance to disinfection undoubtedly contribute to their success as foodborne disease agents. The purpose of this study was to compare the efficacy of three commonly used disinfectant active ingredients against representative HuNoV strains and cultivable surrogates. Ethanol (50, 70, and 90%), sodium hypochlorite (5, 75, 250, 500, and 1,000 ppm), and a quaternary ammonium compound blend (at 0.1 x, 1.0 x, and 10 x concentrations) were evaluated against two norovirus (NoV) genogroup II strains (GII.2 and GII.4) and two surrogates (feline calicivirus [FCV] and murine norovirus [MNV-1]). Virucidal suspension assays (30-s exposure) were conducted in accordance with ASTM International standard E-1052. Virus inactivation was quantified using reverse transcription quantitative PCR targeting the ORFI-ORFII junction (HuNoV), the RNA polymerase region (MNV-1), or the ORFI region (F(:V); infectivity assays were also performed for MNV-1 and FCV. The two HuNoV strains and FCV were relatively resistant to ethanol (<0.5 log inactivation) irrespective of concentration, but MNV-1 was much more susceptible (log inactivation, similar to 2.0 log at higher ethanol concentrations). Both HuNoV strains were more resistant to hypochlorite than were either of the animal surrogates, with the human strains requiring >= 500 ppm of hypochlorite to achieve statistically significant reduction (>= 3.0 log) in virus concentration. All four viruses were resistant to inactivation (<0.5-log reduction) using the quaternary ammonium compound formulation at all concentrations tested. This study is novel in that it clearly demonstrates the relative ineffectiveness of common active disinfectant ingredients against HuNoV and highlights the fact that the cultivable surrogates do not always mimic HuNoV strains.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.4315/0362-028x.jfp-12-532" target="_blank" rel="noreferrer noopener">10.4315/0362-028x.jfp-12-532</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2013
Arbogast J
Biotechnology & Applied Microbiology
enteric viruses
feline calicivirus
Food Science & Technology
gii.4 norovirus
hand sanitizers
in-vitro
Jaykus L A
Journal Article
Journal of Food Protection
Macinga D
murine norovirus
norwalk-like viruses
stainless-steel
surfaces
Tung G
virucidal activity
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1097/00007632-199803010-00020" target="_blank" rel="noreferrer noopener">http://doi.org/10.1097/00007632-199803010-00020</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
634-640
Issue
5
Volume
23
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Title
A name given to the resource
Spine update - Lumbar interbody cages
Publisher
An entity responsible for making the resource available
Spine
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
1998-03
Subject
The topic of the resource
anterior; arthrodesis; bone; cages; carbon; composite; fusion; interface; in-vitro; lumbar spine; material orthopedic implants; model; Neurosciences & Neurology; Orthopedics; wear
Creator
An entity primarily responsible for making the resource
Weiner B K; Fraser R D
Description
An account of the resource
Interbody cage devices, used to assist interbody fusion, are rapidly gaining popularity in the surgical management of chronic low back pain. This update provides a structural classification of commonly used devices and assesses them against a set of clearly defined surgical goals, including ability to correct the existing mechanical deformation, ability to provide mechanical stability, ability to provide a suitable environment for arthrodesis, and ability to limit "built-in" morbidity. In addition, the materials used in the devices are examined regarding their biomechanical, biologic, and radiographic characteristics.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1097/00007632-199803010-00020" target="_blank" rel="noreferrer noopener">10.1097/00007632-199803010-00020</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1998
anterior
arthrodesis
Bone
cages
carbon
composite
Fraser R D
fusion
in-vitro
interface
Journal Article
lumbar spine
material orthopedic implants
model
Neurosciences & Neurology
Orthopedics
Spine
wear
Weiner B K
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1021/jm4004736" target="_blank" rel="noreferrer noopener">http://doi.org/10.1021/jm4004736</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
5306-5320
Issue
13
Volume
56
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Title
A name given to the resource
Synthesis and Anticancer Mechanism Investigation of Dual Hsp27 and Tubulin Inhibitors
Publisher
An entity responsible for making the resource available
Journal of Medicinal Chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-07
Subject
The topic of the resource
breast-cancer; c-dependent activation; cancer cells; chaperone activity; cytochrome-c; heat-shock proteins; in-vitro; nimesulide analog jcc76; pancreatic-cancer; Pharmacology & Pharmacy; self-association
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Zhong B; Chennamaneni S; Lama R; Yi X; Geldenhuys W J; Pink J J; Dowlati A; Xu Y; Zhou A M; Su B
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Heat shock protein 27 (Hsp27) is a chaperone protein, and its expression is increased in response to various stress stimuli including anticancer chemotherapy, which allows the cells to survive and causes drug resistance. We previously identified lead compounds that bound to Hsp27 and tubulin via proteomic approaches. Systematic ligand based optimization in the current study significantly increased the cell growth inhibition and apoptosis inducing activities of the compounds. Compared to the lead compounds, one of the new derivatives exhibited much better potency to inhibit tubulin polymerization but a decreased activity to inhibit Hsp27 chaperone function, suggesting that the structural modification dissected the dual targeting effects of the compound. The most potent compounds 20 and 22 exhibited strong cell proliferation inhibitory activities at subnanomolar concentration against 60 human cancer cell lines conducted by Developmental Therapeutic Program at the National Cancer Institute and represented promising candidates for anticancer drug development.
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<a href="http://doi.org/10.1021/jm4004736" target="_blank" rel="noreferrer noopener">10.1021/jm4004736</a>
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Journal Article
2013
breast-cancer
c-dependent activation
cancer cells
chaperone activity
Chennamaneni S
cytochrome-c
Dowlati A
Geldenhuys W J
Heat-Shock Proteins
in-vitro
Journal Article
Journal of medicinal chemistry
Lama R
nimesulide analog jcc76
pancreatic-cancer
Pharmacology & Pharmacy
Pink J J
self-association
Su B
Xu Y
Yi X
Zhong B
Zhou A M