1
40
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Text
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URL Address
<a href="http://doi.org/10.1093/rheumatology/kes363" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/rheumatology/kes363</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
998-1008
Issue
6
Volume
52
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Dublin Core
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Title
A name given to the resource
Delphinidin Inhibits Il-1 Beta-induced Activation Of Nf-kappa B By Modulating The Phosphorylation Of Irak-1(ser376) In Human Articular Chondrocytes
Publisher
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Rheumatology
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-06
Subject
The topic of the resource
chondrocytes; COX-2; delphinidin; down-regulation; factor-alpha; gene-expression; human osteoarthritis chondrocytes; IL-1 beta; in-vitro; interleukin-1; kinase; nf-kappa-b; osteoarthritis; oxidative stress; PGE(2); rheumatoid-arthritis; Rheumatology; tumor-necrosis-factor
Creator
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Haseeb A; Chen D X; Haqqi T M
Description
An account of the resource
Objective. In OA, there is enhanced expression of pro-inflammatory cytokines such as IL-1 beta in the affected joint. Delphinidin, an anthocyanidin found in pigmented fruits and vegetables, has been shown to possess anti-inflammatory and antioxidant properties. In the present study we determined whether delphinidin would inhibit the IL-1 beta-induced activation of NF-kappa B in human chondrocytes and determined the mechanism of its action. Methods. PGE(2) levels and activation of NF-kappa B p65 in human OA chondrocytes were determined by ELISA-based assays. Protein expression of cyclo-oxygenase-2 (COX-2) and phosphorylation of kinases was determined by western immunoblotting. Expression level of mRNAs was determined by TaqMan assays. Results. Delphinidin inhibited IL-1 beta-induced expression of COX-2 and production of PGE(2) in human chondrocytes. Delphinidin also inhibited IL-1 beta-mediated phosphorylation of IL-1 receptor-associated kinase-1(Ser376), phosphorylation of IKK alpha/beta, expression of IKK beta, degradation of I kappa B alpha, and activation and nuclear translocation of NF-kappa B/p65. Phosphorylation of TGF-beta-activated kinase 1 was not observed but NF-kappa B-inducing kinase (NIK) was phosphorylated and phosphorylation of NIK was blocked by delphinidin in IL-1 beta-treated human chondrocytes. Conclusion. These data identify delphinidin as a novel inhibitor of IL-1 beta-induced production of cartilage-degrading molecule PGE(2) via inhibition of COX-2 expression and provide new insight into the mechanism of its action. Our results also identify inhibition of IRAK1(Ser376) phosphorylation by delphinidin in IL-1 beta-induced activation of NF-kappa B in human chondrocytes. Given the important role played by IL-1 beta-induced NF-kappa B activation, COX-2 expression and PGE(2) production in OA, our results may have important implications for the development of novel therapeutic strategies for the prevention/treatment of OA.
Identifier
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<a href="http://doi.org/10.1093/rheumatology/kes363" target="_blank" rel="noreferrer noopener">10.1093/rheumatology/kes363</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2013
Chen D X
Chondrocytes
COX-2
delphinidin
Down-Regulation
factor-alpha
gene-expression
Haqqi T M
Haseeb A
human osteoarthritis chondrocytes
IL-1 beta
in-vitro
interleukin-1
Journal Article or Conference Abstract Publication
Kinase
nf-kappa-b
Osteoarthritis
Oxidative Stress
PGE(2)
rheumatoid-arthritis
Rheumatology
tumor-necrosis-factor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/1529-0131(200007)43:7%3C1580::aid-anr23%3E3.3.co;2-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/1529-0131(200007)43:7%3C1580::aid-anr23%3E3.3.co;2-7</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1580-1590
Issue
7
Volume
43
Search for Full-text
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Response of engineered cartilage tissue to biochemical agents as studied by proton magnetic resonance microscopy
Publisher
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Arthritis and Rheumatism
Date
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2000
2000-07
Subject
The topic of the resource
acid; anterior cruciate ligament; canine articular-cartilage; chondrocytes; interleukin-1; matrix; nmr; osteoarthritis; Rheumatology; self-diffusion; water
Creator
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Potter K; Butler J J; Horton W E; Spencer R G S
Description
An account of the resource
Objective, To test the hypothesis that magnetic resonance imaging (MRI) results correlate with the biochemical composition of cartilage matrix and can therefore be used to evaluate natural tissue development and the effects of biologic interventions. Methods. Chondrocytes harvested from day-16 chick embryo sterna were inoculated into an MRI-compatible hollow-fiber bioreactor, The tissue that formed over a period of 2-4 weeks was studied biochemically, histologically, and with MRI. Besides natural development, the response of the tissue to administration of retinoic acid, interleukin-1 beta (IL-1 beta), and daily dosing with ascorbic acid was studied. Results. Tissue wet and dry weight, glycosaminoglycan (GAG) content, and collagen content all increased with development time, while tissue hydration decreased. The administration of retinoic acid resulted in a significant reduction in tissue wet weight, proteoglycan content, and cell number and an increase in hydration as compared with controls. Daily dosing with ascorbic acid increased tissue collagen content significantly compared with controls, while the administration of IL-1 beta resulted in increased proteoglycan content. The water proton longitudinal and transverse relaxation rates correlated well with GAG and collagen concentrations of the matrix as well as with tissue hydration. In contrast, the magnetization transfer value for the tissue correlated only with total collagen, Finally, the self-coefficient of water correlated with tissue diffusion hydration. Conclusion. Parameters derived from MR images obtained noninvasively can be used to quantitatively assess the composition of cartilage tissue generated in a bioreactor. We conclude that MRI is a promising modality for the assessment of certain biochemical properties of cartilage in a wide variety of settings.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/1529-0131(200007)43:7%3C1580::aid-anr23%3E3.3.co;2-7" target="_blank" rel="noreferrer noopener">10.1002/1529-0131(200007)43:7%3C1580::aid-anr23%3E3.3.co;2-7</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2000
acid
anterior cruciate ligament
Arthritis and rheumatism
Butler J J
canine articular-cartilage
Chondrocytes
Horton W E
interleukin-1
Journal Article
matrix
nmr
Osteoarthritis
Potter K
Rheumatology
self-diffusion
Spencer R G S
Water