Amphiphilic Gels With Controlled Mesh Dimensions For Insulin Delivery
drug release; islets; methacrylate)-1-polyisobutylene; networks; xenotransplantation
Kennedy J P; Fenyvesi G; Na S; Keszler B; Rosenthal K S
Polymer Gels: Fundamentals and Applications
2003
2003
Book Chapter
n/a
Amphiphilic Membranes With Controlled Mesh Dimensions For Insulin Delivery
immunosuppression; islets; networks; Polymer Science; porcine; release; xenotransplantation
A series of amphiphilic networks (membranes), consisting of hydrophilic poly(N,N-dimethylacrylamide) (PDMAAM) main chains crosslinked by hydrophobic telechelic polyisobutylene di- and trimethacrylates (MA-PIB-MA or circle divide (PEB-MA)(3)) have been synthesized and used for the preparation of thin-walled tubules suitable for the immunoisolation of porcine islets. The molecular weight cut-off (MWCO) ranges, insulin and glucose diffusion coefficients and permeabilities of various membranes have been determined. The molecular weight of the PDMAAM moiety between two hydrophobic crosslinking points M-c,M-hydrophilic) controls permeability, which in turn can be controlled by synthesis conditions. The strengths and elongations of water-swollen membranes crosslinked with circle divide (PIB-MA)(3) are superior by a factor of about two to those prepared with MA-PIB-MA. Based on the values from these experiments, a well-defined membrane prepared with circle divide (PEB-MA)(3) was selected and used to encapsulate porcine islet cells. Gratifyingly, the encapsulated islet cells remain functional and viable, and cells within the tubule release insulin upon glucose challenge. These in vitro experiments are sufficiently promising to encourage us to continue our studies to develop a bioartificial pancreas.
Kennedy J P; Fenyvesi G; Levy R P; Rosenthal K S
Macromolecular Symposia
2001
2001-06
Journal Article or Conference Abstract Publication
n/a