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Text
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URL Address
<a href="http://doi.org/10.1152/ajpendo.00457.2017" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpendo.00457.2017</a>
Pages
E416–E424
Issue
3
Volume
315
Dublin Core
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Title
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Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS.
Publisher
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American journal of physiology. Endocrinology and metabolism
Date
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2018
2018-09
Subject
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dyslipidemia; insulin resistance; kinetics; lipid turnover; mass spectrometry
Creator
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Chen Ying; Berejnaia Olga; Liu Jinqi; Wang Sheng-Ping; Daurio Natalie A; Yin Wu; Mayoral Rafael; Petrov Aleksandr; Kasumov Takhar; Zhang Guo-Fang; Previs Stephen F; Kelley David E; McLaren David G
Description
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Numerous studies have implicated dyslipidemia as a key factor in mediating insulin resistance. Ceramides have received special attention since their levels are inversely associated with normal insulin signaling and positively associated with factors that are involved in cardiometabolic disease. Despite the growing literature surrounding ceramide biology, there are limited data regarding the activity of ceramide synthesis and turnover in vivo. Herein, we demonstrate the ability to measure ceramide kinetics by coupling the administration of [(2)H]water with LC-MS/MS analyses. As a "proof-of-concept" we determined the effect of a diet-induced alteration on ceramide flux; studies also examined the effect of myriocin (a known inhibitor of serine palmitoyltransferase, the first step in sphingosine biosynthesis). Our data suggest that one can estimate ceramide synthesis and draw conclusions regarding the source of fatty acids; we discuss caveats in regards to method development in this area.
Identifier
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<a href="http://doi.org/10.1152/ajpendo.00457.2017" target="_blank" rel="noreferrer noopener">10.1152/ajpendo.00457.2017</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2018
American journal of physiology. Endocrinology and metabolism
Berejnaia Olga
Chen Ying
Daurio Natalie A
Department of Pharmaceutical Sciences
dyslipidemia
Insulin Resistance
Kasumov Takhar
Kelley David E
Kinetics
lipid turnover
Liu Jinqi
Mass spectrometry
Mayoral Rafael
McLaren David G
NEOMED College of Pharmacy
Petrov Aleksandr
Previs Stephen F
Wang Sheng-Ping
Yin Wu
Zhang Guo-Fang