1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1016/j.ab.2017.03.009" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ab.2017.03.009</a> Pages 29–32 Volume 526 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource An effective and efficient method of transfecting primary human chondrocytes in suspension. Publisher An entity responsible for making the resource available Analytical biochemistry Date A point or period of time associated with an event in the lifecycle of the resource 2017 2017-06 Subject The topic of the resource *Chondrocytes; *Osteoarthritis; *siRNA; *Transfection; Aggrecans/genetics; Articular/metabolism/pathology; Cartilage; Cells; Chondrocytes/cytology/*metabolism; Collagen Type II/genetics; Cultured; Humans; Messenger/*genetics; mRNA Cleavage and Polyadenylation Factors/antagonists & inhibitors/genetics; Nucleotidyltransferases/antagonists & inhibitors/genetics; Plasmids/*genetics; RNA; Small Interfering/*genetics; Transfection/*methods Creator An entity primarily responsible for making the resource Makki Mohammad Shahidul; Akhtar Nahid; Haqqi Tariq M Description An account of the resource Human chondrocytes accumulate an ECM-rich matrix by secreting matrix macromolecules during monolayer culture, which makes them difficult to transfect efficiently. Here we report a non-viral based protocol to transfect the primary human chondrocytes with high efficiency in suspension. Chondrocyte cultures were digested using Pronase and Collagenase and transfected in suspension. Transfection efficiencies of more than 80% were achieved routinely using the protocol described. The viability of siRNA transfected or un-transfected chondrocytes was not affected and resulted in 80-90% knockdown of the target mRNA levels. This protocol may be useful in gene knockdown, and ectopic overexpression studies in chondrocytes. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1016/j.ab.2017.03.009" target="_blank" rel="noreferrer noopener">10.1016/j.ab.2017.03.009</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). *CHONDROCYTES *OSTEOARTHRITIS *siRNA *Transfection 2017 Aggrecans/genetics Akhtar Nahid Analytical biochemistry Articular/metabolism/pathology Cartilage Cells Chondrocytes/cytology/*metabolism Collagen Type II/genetics Cultured Department of Anatomy & Neurobiology Haqqi Tariq M Humans Makki Mohammad Shahidul Messenger/*genetics mRNA Cleavage and Polyadenylation Factors/antagonists & inhibitors/genetics NEOMED College of Medicine Nucleotidyltransferases/antagonists & inhibitors/genetics Plasmids/*genetics RNA Small Interfering/*genetics Transfection/*methods