1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1016/s0006-3495(01)76113-9" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0006-3495(01)76113-9</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 1406-1416 Issue 3 Volume 80 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Determination of membrane immersion depth with O-2: A high-pressure F-19 NMR study Publisher An entity responsible for making the resource available Biophysical Journal Date A point or period of time associated with an event in the lifecycle of the resource 2001 2001-03 Subject The topic of the resource Biophysics; cholesterol; conformational-changes; dynamics; lipid bilayers; micelles; nuclear magnetic-resonance; phospholipid-bilayers; protein-structure; solid-state nmr; x-ray-diffraction Creator An entity primarily responsible for making the resource Prosser R S; Luchette P A; Westerman P W; Rozek A; Hancock R E W Description An account of the resource Oxygen is known to partition with an increasing concentration gradient toward the hydrophobic membrane interior. At partial pressures (P-O2) of 100 Atm or more, this concentration gradient is sufficient to induce paramagnetic effects that depend sensitively on membrane immersion depth. This effect is demonstrated for the fluorine nucleus by depth-dependent: paramagnetic shifts and spin-lattice relaxation rates, using a fluorinated detergent, CF3(CF2)(5)C2H4-O-maltose (TFOM), reconstituted into a lipid bilayer model membrane system, To interpret the spin-lattice relaxation rates (R-1(P)) in terms of a precise immersion depth, two specifically fluorinated cholesterol species (6-fluorocholesterol and 25-fluorocholesterol), whose membrane immersion depths were independently estimated, were studied by F-19 NMR. The paramagnetic relaxation rates, R-1(P), of the cholesterol species were then used to parameterize a Gaussian profile that directly relates R-1(P) to immersion depth z.: This same Gaussian curve could then be used to determine the membrane immersion depth of all six fluorinated chain positions of TFOM and of two adjacent residues of specifically fluorinated analogs of the antibacterial peptide indolicidin. The potential of this method for determination of immersion depth and topology of membrane proteins is discussed. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1016/s0006-3495(01)76113-9" target="_blank" rel="noreferrer noopener">10.1016/s0006-3495(01)76113-9</a> Format The file format, physical medium, or dimensions of the resource Journal Article 2001 Biophysical Journal Biophysics Cholesterol conformational-changes dynamics Hancock R E W Journal Article Lipid Bilayers Luchette P A micelles nuclear magnetic-resonance phospholipid-bilayers Prosser R S protein-structure Rozek A solid-state nmr Westerman P W x-ray-diffraction