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Text
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URL Address
<a href="http://doi.org/10.1080/03008200701692685" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/03008200701692685</a>
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Pages
316-323
Issue
6
Volume
48
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Title
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Analysis of human osteoarthritic connective tissue by laser capture microdissection and QRT-PCR
Publisher
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Connective Tissue Research
Date
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2007
2007
Subject
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adult articular-cartilage; aggrecan gene expression; arthroplasty; cartilage; Cell Biology; chondrocytes; disease; gene-expression; human osteoarthritis; in-vitro; knee; laser capture; microdissection; molecular analysis; Orthopedics; QRT-PCR; quality-of-life; replacement surgery; skeletal tissues; type II collagen
Creator
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Scharschmidt T; Jacquet R; Laskovski J; Lowder E; Weiner S; Landis W J
Description
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Gene expression levels for type II collagen and aggrecan have been determined as potential measures and disease markers of human osteoarthritis in patients undergoing total knee arthroplasty. In this regard, specimens of affected articular cartilage obtained intraoperatively at the time of surgery were placed in RNAlater (TM) to maintain RNA integrity and subsequently frozen-sectioned. Individual or small numbers of chondrocytes were isolated by laser capture microdissection and their total RNA was extracted and analyzed by quantitative reverse transcription-polymerase chain reaction. Results indicate that type II collagen and aggrecan mRNA expression from specific cells in osteoarthritic tissues are detectable and reproducible using these approaches. Our work is the first to demonstrate successful isolation of RNA limited to chondrocytes comprising small quantities of human osteoarthritic material. The study presents a new avenue by which the disease and its progression may be critically assayed.
Identifier
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<a href="http://doi.org/10.1080/03008200701692685" target="_blank" rel="noreferrer noopener">10.1080/03008200701692685</a>
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Journal Article
2007
adult articular-cartilage
aggrecan gene expression
Arthroplasty
Cartilage
Cell Biology
Chondrocytes
Connective tissue research
Disease
gene-expression
human osteoarthritis
in-vitro
Jacquet R
Journal Article
Knee
Landis W J
laser capture
Laskovski J
Lowder E
Microdissection
molecular analysis
Orthopedics
QRT-PCR
quality-of-life
replacement surgery
Scharschmidt T
skeletal tissues
type II collagen
Weiner S