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<a href="http://doi.org/10.1046/j.1469-7580.1998.19210013.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1046/j.1469-7580.1998.19210013.x</a>
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Pages
13-23
Volume
192
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Title
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Ultrastructural and cytochemical evaluation of sepsis-induced changes in the rat pulmonary intravascular mononuclear phagocytes
Publisher
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Journal of Anatomy
Date
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1998
1998-01
Subject
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acid-phosphatase; Anatomy & Morphology; Blood; cells; coagulation; differentiation; endotoxin; expression; host defence; human macrophage; lung uptake; monocytes; mononuclear phagocyte system; surface-coat
Creator
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Singh B; Doane K J; Niehaus G D
Description
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Sepsis stimulates an increase in the number and activity of mononuclear phagocytes in systemic host-defence organs. The present study was conducted to define the ultrastructural and cytochemical characteristics of the mononuclear phagocytes that sequester in the lung microvasculature of septic rats, Fourteen rats were challenged with a single intraperitoneal injection of saline (0.5 ml/100 g), E. coli (2 x 10(7)/100 g) or glucan (4 mg/100 g), and euthanased 2, 4, or 7 d later. The lungs were inflation fixed and processed for transmission electron microscopy. Cellular morphology was used to identify the intravascular mononuclear phagocytes and acid phosphatase (AcPase) expression was monitored as an index of cellular differentiation and activation. Control rats contained a limited number of monocytes in the pulmonary vasculature. In contrast, large numbers of activated mononuclear phagocytes were seen in the microvasculature within 48 h of treatment with either microbial product. The recruited pulmonary intravascular mononuclear phagocytes (PIMP) exhibited AcPase-reactive Golgi complexes, accumulation of secretory vesicles and other features of cell activation consistent with enhanced biosynthetic activity. Subsequent electron microscopy, conducted 4 and 7 d posttreatment, suggested that a progressive decline in the number and activity of PIMPs then occurred. In order to quantify the sepsis-induced accumulation of AcPase-positive PIMP, the experimental challenges were repeated in 11 rats and, 48 h later, tissue samples were evaluated by light microscopy for tartrate-insensitive acid phosphatase. Control rats exhibited 0.148 +/- 0.107 AcPase-positive PIMP/alveoli. E. coli and glucan challenged animals exhibited significant (P < 0.01) increases in AcPase-positive mononuclear phagocytes, with 0.782+/-0.073 and 0.636+/-0.170 PIMP/alveoli respectively, The results demonstrate that focal sepsis stimulates a significant, but transient, recruitment of activated mononuclear phagocytes into the rat pulmonary microvasculature.
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<a href="http://doi.org/10.1046/j.1469-7580.1998.19210013.x" target="_blank" rel="noreferrer noopener">10.1046/j.1469-7580.1998.19210013.x</a>
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Journal Article
1998
acid-phosphatase
Anatomy & Morphology
Blood
Cells
coagulation
differentiation
Doane K J
endotoxin
expression
host defence
human macrophage
Journal Article
Journal of anatomy
lung uptake
monocytes
mononuclear phagocyte system
Niehaus G D
Singh B
surface-coat