Rodent models for ovarian cancer research
animal models; carcinoma cell-line; epithelial; gene-therapy; immunodeficient mice; luteinizing-hormone; lysophosphatidic acid; metastasis in-vivo; mouse models; neoplasms; nude-mice; Obstetrics & Gynecology; Oncology; ovarian-cancer; p53; review; tumor-associated antigens
Animal models that are biologically and clinically relevant are essential for conducting research to investigate the pathophysiologic progression of disease and to develop diagnostic or therapeutic strategies. Several rodent models that vary in methods of induction allow appropriate in vivo evaluation for ovarian cancer. The types of rodent models discussed include chemically (nonhormonal and hormonal) induced, genetic (knockout and transgenic), xenograft, and syngeneic. A summary of the available rodent models is provided with a discussion of the advantages and disadvantages of each. Optimization and application of these rodent models to future research may benefit the detection and treatment of ovarian cancer.
Stakleff K D S; Von Gruenigen V E
International Journal of Gynecological Cancer
2003
2003-07
Journal Article
<a href="http://doi.org/10.1046/j.1525-1438.2003.13317.x" target="_blank" rel="noreferrer noopener">10.1046/j.1525-1438.2003.13317.x</a>
Measuring bone blood supply in mice using fluorescent microspheres
Angiogenesis; Biochemistry & Molecular Biology; flow measurement; hindlimb ischemia; mouse models; perfusion; rabbits; rats
Fluorescent microspheres are commonly used to assess bone blood supply in large animals, but the technique is not widely used in smaller mammals, as traditional methods such as reference blood sampling, ventilation and catheterization are not easily applied. This protocol describes a viable alternative for measuring bone and organ perfusion in mice using modified fluorescent microsphere techniques. Microspheres are injected directly into the left heart and a reference tissue is used to calculate relative bone and organ blood supply. On the basis of a sample of 15 mice with 5 tissues each, the entire protocol takes 140.5 h to complete from animal preparation through statistical analysis. This timing includes 72 h of mandated pauses for bone decalcification and digestion, as well as 48 h for data analysis. Exclusive of pauses or additional analyses that could increase the time required, this protocol takes 20.5 h bench time to complete.
Serrat M A
Nature Protocols
2009
2009
Journal Article
<a href="http://doi.org/10.1038/nprot.2009.190" target="_blank" rel="noreferrer noopener">10.1038/nprot.2009.190</a>