1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1046/j.1471-4159.2000.0740081.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1046/j.1471-4159.2000.0740081.x</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 81-91 Issue 1 Volume 74 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rat Alpha(1)-macroglobulin Enhances Nerve Growth Factor-promoted Neurite Outgrowth, Trka Phosphorylation, And Gene Expression Of Pheochromocytoma Pc12 Cells Publisher An entity responsible for making the resource available Journal of Neurochemistry Date A point or period of time associated with an event in the lifecycle of the resource 2000 2000-01 Subject The topic of the resource alpha-2-macroglobulin receptor; alpha-macroglobulins; alpha(2)-macroglobulin; Biochemistry & Molecular Biology; caudate putamen; cerebral cortical-neurons; choline-acetyltransferase; delayed-response genes; immediate-early genes; myelin-associated glycoprotein; nerve growth factor receptor; neurite-promoting factor; Neurosciences & Neurology; ngf receptor; protein; signal; signal-transduction; transduction Creator An entity primarily responsible for making the resource Lee P G; Koo P H Description An account of the resource Monoamine-activated human alpha(2)-macroglobulin (alpha(2)M) has been previously demonstrated to inhibit TrkA-, TrkB-, and TrkC-mediated signal transduction. Rat alpha(1),-macroglobulin (alpha(1)M) and alpha(2)M are structural homologues of human alpha(2)M, but rat alpha(1)M is distinctly different from rat alpha(2)M in many ways and its role in the mammalian nervous system is unknown. In this report, monoamine-activated rat alpha(1)M was demonstrated to enhance in a dose-dependent manner nerve growth factor (NGF)-promoted neurite outgrowth in pheochromocytoma PC12 cells. Monoamine-activated alpha(1)M by itself, however, was neither neurotrophic nor mitogenic to PC12 cells. To investigate further its possible mode of action, the ability of monoamine-activated alpha(1)M and normal alpha(1)M to bind and to activate the NGF receptor (TrkA) was investigated. Monoamine-activated alpha(1)M formed a more stable complex with TrkA than normal alpha(1)M, but the binding of mono-amina-activated alpha(1)M to TrkA was adversely affected by prior stimulation of TrkA with NGF. In addition, monoamine-activated alpha(1)M enhanced the NGF-promoted TrkA phosphorylation and up-regulated the expression of NGF-inducible immediate-early genes (c-jun and NGFI-A) and delayed-response genes (SCG10 and transin) in PC12 cells; normal alpha(1)M, in contrast, produced little or no effect, This study demonstrates that alpha(1)M, the constitutive form of ar-macroglobulin in the rat, possesses the ability to promote NGF-mediated differentiation in PC12 cells, possibly via its direct action on TrkA receptors and TrkA-mediated signal transduction and gene expression. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1046/j.1471-4159.2000.0740081.x" target="_blank" rel="noreferrer noopener">10.1046/j.1471-4159.2000.0740081.x</a> Format The file format, physical medium, or dimensions of the resource Journal Article or Conference Abstract Publication 2000 alpha-2-macroglobulin receptor alpha-macroglobulins alpha(2)-macroglobulin Biochemistry & Molecular Biology caudate putamen cerebral cortical-neurons choline-acetyltransferase delayed-response genes immediate-early genes Journal Article or Conference Abstract Publication Journal of neurochemistry Koo P H Lee P G myelin-associated glycoprotein nerve growth factor receptor neurite-promoting factor Neurosciences & Neurology ngf receptor Protein signal signal-transduction Transduction