1
40
8
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1515/mamm.1999.63.2.159" target="_blank" rel="noreferrer noopener">http://doi.org/10.1515/mamm.1999.63.2.159</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
159-166
Issue
2
Volume
63
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bat Salivary Proteins Segregate According To Diet
Publisher
An entity responsible for making the resource available
Mammalia
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999
Subject
The topic of the resource
carbonic-anhydrase; cat; glands; mammals; parotid-saliva; polyacrylamide gel-electrophoresis; proline-rich proteins; purification; rat; secretion; stimulation; Zoology
Creator
An entity primarily responsible for making the resource
Dumont E R; Etzel K; Hempel D
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1515/mamm.1999.63.2.159" target="_blank" rel="noreferrer noopener">10.1515/mamm.1999.63.2.159</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1999
carbonic-anhydrase
cat
Dumont E R
Etzel K
glands
Hempel D
Mammalia
Mammals
parotid-saliva
polyacrylamide gel-electrophoresis
proline-rich proteins
purification
rat
secretion
Stimulation
Zoology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
483-491
Volume
206
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY OF CHOLESTEROL 7-ALPHA-HYDROXYLASE
Publisher
An entity responsible for making the resource available
Methods in Enzymology
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991
Subject
The topic of the resource
Biochemistry & Molecular Biology; cloning; enzyme; purification; rat-liver microsomes; cholesterol 7-alpha-hydroxylase
Creator
An entity primarily responsible for making the resource
Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1991
Biochemistry & Molecular Biology
Chiang J Y L
cholesterol 7-alpha-hydroxylase
Cloning
enzyme
Journal Article or Conference Abstract Publication
Methods in Enzymology
purification
rat-liver microsomes
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1172/jci119502" target="_blank" rel="noreferrer noopener">http://doi.org/10.1172/jci119502</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
11-17
Issue
1
Volume
100
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Title
A name given to the resource
Kallistatin is a potent new vasodilator
Publisher
An entity responsible for making the resource available
Journal of Clinical Investigation
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-07
Subject
The topic of the resource
Research & Experimental Medicine; expression; purification; endothelium; vasopressin; inhibitor; bradykinin; blood pressure; human tissue kallikrein; hypertensive rats; kallikrein; kallikrein-binding protein; kallistatin; kinins; renal pressure; vasorelaxation
Creator
An entity primarily responsible for making the resource
Chao J L; Stallone J N; Liang Y M; Chen L M; Wang D Z; Chao L
Description
An account of the resource
Kallistatin is a serine proteinase inhibitor which binds to tissue kallikrein and inhibits its activity, The aim of this study is to evaluate if kallistatin has a direct effect on the vasculature and on blood pressure homeostasis. We found that an intravenous bolus injection of human kallistatin caused a rapid, potent, and transient reduction of mean arterial blood pressure in anesthetized rats. Infusion of purified kallistatin (0.07-1.42 nmoVkg) into cannulated rat jugular vein produced a 20-85 mmHg reduction of blood pressure in a dose-dependent manner. Hoe 140, a bradykinin B-2-receptor antagonist, had no effect on the hypotensive effect of kallistatin yet it abolished the blood pressure-lowering effect of kinin and kallikrein. Relaxation of isolated aortic rings by kallistatin was observed in the presence (ED50 of 3.4 x 10(-9) M) and in the absence of endothelium (ED50 of 10(-9) M). Rat kallikrein-binding protein, but not kinin or kallikrein, induced vascular relaxation of aortic rings. Neither Hoe 140 nor N-omega-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor, affected vasorelaxation induced by kallistatin. Kallistatin also caused dose-dependent vasodilation of the renal vasculature in the isolated, perfused rat kidney, Specific kallistatin-binding sites were identified in rat aorta by Scatchard plot analysis with a K-d of 0.25+/-0.07 nM and maximal binding capacity of 47.9+/-10.4 fmol/mg protein (mean+/-SEM, n = 3). These results indicate that kallistatin is a potent vasodilator which may function directly through a vascular smooth muscle mechanism independent of an endothelial bradykinin receptor. This study introduces the potential significance of kallistatin in directly regulating blood pressure to reduce hypertension.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1172/jci119502" target="_blank" rel="noreferrer noopener">10.1172/jci119502</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1997
Blood Pressure
bradykinin
Chao J L
Chao L
Chen L M
Endothelium
expression
human tissue kallikrein
hypertensive rats
inhibitor
Journal Article or Conference Abstract Publication
Journal of Clinical Investigation
kallikrein
kallikrein-binding protein
kallistatin
kinins
Liang Y M
purification
renal pressure
Research & Experimental Medicine
Stallone J N
vasopressin
vasorelaxation
Wang D Z
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1111/j.1432-1033.1993.tb18082.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1111/j.1432-1033.1993.tb18082.x</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
705-710
Issue
3
Volume
215
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Title
A name given to the resource
CHOLESTEROL 7-ALPHA-HYDROXYLASE IS UP-REGULATED BY THE COMPETITIVE INHIBITOR 7-OXOCHOLESTEROL IN RAT-LIVER
Publisher
An entity responsible for making the resource available
European Journal of Biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-08
Subject
The topic of the resource
Biochemistry & Molecular Biology; expression; cloning; dietary-cholesterol; purification; messenger-rna; circadian-rhythm; reductase; microsomes; sequence; serum-cholesterol
Creator
An entity primarily responsible for making the resource
Breuer O; Sudjanasugiaman E; Eggertsen G; Chiang J Y L; Bjorkhem I
Description
An account of the resource
Rats of the Sprague-Dawley strain were infused intravenously with a fat emulsion (Intralipid, trademark of Kabi Pharmacia, Uppsala, Sweden) containing 7-oxocholesterol. This resulted in an increased cholesterol 7alpha-hydroxylase activity in liver microsomes as compared to controls and was accompanied by increased levels of cholesterol 7alpha-hydroxylase mRNA and microsomal cholesterol 7alpha-hydroxylase protein. Rats were also fed a cholestyramine-supplemented diet and infused with 7-oxocholesterol. These animals excreted about half as much bile acids in faeces as cholestyramine-fed controls. Addition of 7-oxocholesterol to liver microsomes from normal rats in amounts corresponding to those present in microsomes from 7-oxocholesterol-treated rats inhibited the cholesterol 7alpha-hydroxylase activity by about 75%. Cholesterol induced a type-I binding spectrum when added to a purified bacterial-expressed cholesterol 7alpha-hydroxylase (P-450c7DELTA2-24). 7-Oxocholesterol competitively inhibited the cholesterol binding spectrum, while 7beta-hydroxycholesterol did not interfere with binding of cholesterol to the enzyme. It is concluded that treatment with the competitive inhibitor 7-oxocholesterol leads to a reduced bile acid biosynthesis and, as a consequence of reduced bile acid inhibition, a compensatory increase in cholesterol 7alpha-hydroxylase synthesis. The high enzyme activity measured in microsomal preparations from 7-oxocholesterol-treated rats may be due to a continuous conversion of 7-oxocholesterol into less inhibitory metabolites, e.g. 7beta-hydroxycholesterol. The latter compound was found in high concentrations in liver microsomes from rats treated with 7-oxocholesterol. The physiological importance of these results is discussed in relation to the previous findings that 7-oxocholesterol is accumulated in liver after cholesterol feeding and that 7-oxocholesterol is formed from cholesterol during lipid peroxidation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1111/j.1432-1033.1993.tb18082.x" target="_blank" rel="noreferrer noopener">10.1111/j.1432-1033.1993.tb18082.x</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1993
Biochemistry & Molecular Biology
Bjorkhem I
Breuer O
Chiang J Y L
circadian-rhythm
Cloning
dietary-cholesterol
Eggertsen G
European Journal of Biochemistry
expression
Journal Article or Conference Abstract Publication
messenger-rna
Microsomes
purification
reductase
sequence
serum-cholesterol
Sudjanasugiaman E
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0003-9861(95)90012-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0003-9861(95)90012-8</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
289-296
Issue
2
Volume
320
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
FATTY-ACID DISCRIMINATION AND OMEGA-HYDROXYLATION BY CYTOCHROME-P450 4A1 AND A CYTOCHROME P4504A1/NADPH-P450 REDUCTASE FUSION PROTEIN
Publisher
An entity responsible for making the resource available
Archives of Biochemistry and Biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-07
Subject
The topic of the resource
Biochemistry & Molecular Biology; Biophysics; catalytic properties; cytochrome p450 4a1; escherichia-coli; expression; fusion protein; gene family; inhibition; lauric acid; mechanisms; omega-hydroxylation; purification
Creator
An entity primarily responsible for making the resource
Alterman M A; Chaurasia C S; Lu P; Hardwick J P; Hanzlik R P
Description
An account of the resource
The omega-hydroxylation of fatty acids by certain cytochrome P450 enzymes shows a degree of chain-length and regiospecificity which is remarkable in view of the conformational flexibility of these substrates, the strong similarity in properties among homologs, and the lack of polar groups (other than the carboxy terminus) with which to guide and strengthen enzyme-substrate interactions, To investigate the chemical basis for these features of omega-hydroxylation we designed and synthesized a series of lauric acid analogs and evaluated them as substrates and inhibitors of omega-hydroxylation catalyzed by cytochrome P4504A1 and a cytochrome P450 4A1/NADPH-P450 reductase fusion protein, Among n-alkanoic acids, lauric acid was found to have the optimum chain length for the fusion protein, as it does for native cytochrome P450 4A1, With both enzymes, chain shortening caused a precipitous drop in turnover while chain lengthening caused a gradual drop in turnover, The fusion protein omega-hydroxylated methyl laurate and lauryl alcohol about 1/10th as efficiently as lauric acid, but it did not hydroxylate lauramide, 10-Methoxydecanoic acid underwent O-demethylation (via omega-hydroxylation). The branched substrate 11-methyllauric acid was hydroxylated efficiently and selectively at the omega-position, In contrast, the cyclopropyl analog 11,12-methanolauric acid was not detectably hydroxylated, although it induced Type I binding spectrum and inhibited lauric acid omega-hydroxylation by 43% at equimolar concentrations, omega-(Imidazolyl)-decanoic acid induced a Type II heme-binding spectrum and was an especially potent inhibitor of lauric acid hydroxylation, Collectively these data suggest that the active site of cytochrome P450 4A1 has an elongated tubular shape of definite length (ca, 14 Angstrom) with a recognition site for polar groups (including but not limited to carboxyl) at its entrance and the (oxo)heme group at its terminus, (C) 1995 Academic Press, Inc.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0003-9861(95)90012-8" target="_blank" rel="noreferrer noopener">10.1016/0003-9861(95)90012-8</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1995
Alterman M A
Archives of biochemistry and biophysics
Biochemistry & Molecular Biology
Biophysics
catalytic properties
Chaurasia C S
cytochrome p450 4a1
escherichia-coli
expression
fusion protein
gene family
Hanzlik R P
Hardwick J P
inhibition
Journal Article or Conference Abstract Publication
lauric acid
Lu P
mechanisms
omega-hydroxylation
purification
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0166-3542(97)00040-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0166-3542(97)00040-5</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
63-72
Issue
1
Volume
36
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Title
A name given to the resource
The antiviral xanthate compound D609 inhibits herpes simplex virus type 1 replication and protein phosphorylation
Publisher
An entity responsible for making the resource available
Antiviral Research
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-09
Subject
The topic of the resource
antiviral drug; cells; culture; D609; gene; growth; HSV-1; identification; interruption; kinase; Pharmacology & Pharmacy; phosphorylation; protein; protein-kinase; pseudorabies virus; purification; us3; US3 PK; Virology
Creator
An entity primarily responsible for making the resource
Walro D G; Rosenthal K S
Description
An account of the resource
The mechanism of antiviral action of tricyclodecan-9-yl-xanthogenate (D609) was investigated in vitro. D609 inhibited herpes simplex virus type 1 (HSV-1) replication without apparent cytotoxicity. It reduced phosphorylation of virus-infected cell polypeptides and inhibited the HSV-1 encoded protein kinase (US3 PK) and, to a lesser extent, cellular protein kinase C in vitro. Virus production was reduced by D609 at concentrations greater than 3.8 mu M, With complete inhibition at 75.2 mu M at an MOI of 1 PFU/cell or less. Addition of D609 could be delayed until 7 h post-infection and still inhibit virus replication. Phosphorylation of infected cell viral polypeptides of 34 (similar molecular weight to the substrate of the viral US3 protein kinase) and 69 kDa was inhibited at 18.4 mu M Treatment of infected or uninfected cells with 37.6 mu M D609 reduced protein phosphorylation to background levels. A concentration of 1.9 mu M D609 in vitro inhibited the viral US3-encoded PK, which had been purified from infected cell lysates by affinity chromatography and identified by specific antibody. Purified cellular protein kinase C was inhibited at 75.2 mu M D609 whereas other cellular kinases including casein kinase 1 and cAMP dependent kinase were not inhibited at concentrations as high as 188 mu M D609. Collectively these data indicate that the mechanism of antiviral action of D609 is by inhibition of protein kinases and protein phosphorylation affecting a late step in HSV replication. (C) 1997 Elsevier Science B.V.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0166-3542(97)00040-5" target="_blank" rel="noreferrer noopener">10.1016/s0166-3542(97)00040-5</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1997
antiviral drug
Antiviral research
Cells
Culture
D609
gene
growth
HSV-1
identification
interruption
Journal Article
Kinase
Pharmacology & Pharmacy
Phosphorylation
Protein
protein-kinase
pseudorabies virus
purification
Rosenthal K S
us3
US3 PK
Virology
Walro D G
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
241-249
Issue
4
Volume
17
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Specificity of cDNA-expressed human and rodent cytochrome P450s in the oxidative metabolism of the potent carcinogen 7,12-dimethylbenz a anthracene
Publisher
An entity responsible for making the resource available
Molecular Carcinogenesis
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-12
Subject
The topic of the resource
12-dimethylbenz(a)anthracene; 12-dimethylbenz(a)anthracene; 12-dimethylbenzanthracene metabolites; 7; binding; Biochemistry & Molecular Biology; cDNA expression; chromatography; cytochrome P450; high-performance liquid; human-tissues; hydrocarbons; liver; lung-cancer; metabolism of polycyclic aromatic; microsomes; mouse skin; Oncology; purification; rat; vaccinia virus
Creator
An entity primarily responsible for making the resource
Shou M G; Korzekwa K R; Krausz K W; Buters J T M; Grogan J; Goldfarb I; Hardwick J P; Gonzalez F J; Gelboin H V
Description
An account of the resource
7,12-Dimethylbenz[a]anthracene (DMBA), a potent carcinogen, requires metabolic activation by cytochrome P450s (P450s) to electrophilic metabolites that result in DNA modification, mutagenicity, and carcinogenicity. In this study, we used eight human forms, four rodent forms, and one rabbit form of P450 expressed from recombinant vaccinia or baculovirus vectors to define their specificity for metabolizing DMBA. Of the eight human P450s, 1A1 was the most active (specific activity = 14.7 nmol/min/nmol of P450) in total metabolism of DMBA and showed approximately 6- to 33-fold more activity than other P450s. 2B6, 2C9, and 1A2 were also capable of metabolizing DMBA (2.0-2.5 nmol/min/nmol of P450), whereas 2C8, 2E1, 3A4, and 3A5 exhibited relatively low activities. Among animal P450s, mouse 1A1 exhibited activity similar to that of human 1A1 and had 5.0- to 37-fold more activity than other rodent and rabbit P450s. In regard to enzyme regioselectivity, most human and rodent P450s predominantly formed the 8,9-diol, but human 2B6 and rat 2B1 preferentially formed the 5,6-diol. In the production of monohydroxymethyl metabolites, all the enzymes yielded more 7-hydroxymethyl-12-methylbenz[a]anthracene (7HOM12MBA) than 12-hydroxymethyl-7-methylbenz[a]anthracene (7M12HOMBA), except for human 1A1, which presented the reverse selectivity. Human liver microsomes from 10 organ donors were shown to metabolize DM BA and in most circumstances generated the meta belie profile DM BA trans-8,9-d dihydrodiol > 7HOM12MBA greater than or equal to DMBA trans-5,6-dihydrodiol greater than or equal to 7,12-dihydroxymethylbenz[a]anthracene > 7M12HOMBA > DMBA trans-3,4-dihydrodiol. Thus, the combined activity of hepatic microsomal 2C9, 1A2, and 2B6 may contribute to the metabolic activation and the metabolism of DMBA in normal human liver. (C) 1996 Wiley-Liss, Inc.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g" target="_blank" rel="noreferrer noopener">10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
12-dimethylbenz(a)anthracene
12-dimethylbenzanthracene metabolites
1996
7
Binding
Biochemistry & Molecular Biology
Buters J T M
cDNA expression
Chromatography
cytochrome P450
Gelboin H V
Goldfarb I
Gonzalez F J
Grogan J
Hardwick J P
high-performance liquid
human-tissues
Hydrocarbons
Journal Article
Korzekwa K R
Krausz K W
Liver
lung-cancer
metabolism of polycyclic aromatic
Microsomes
Molecular carcinogenesis
mouse skin
oncology
purification
rat
Shou M G
vaccinia virus
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1053/jhep.1996.v24.pm0008938182" target="_blank" rel="noreferrer noopener">http://doi.org/10.1053/jhep.1996.v24.pm0008938182</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1468-1474
Issue
6
Volume
24
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cholesterol 7 alpha-hydroxylase activities from human and rat liver are modulated in vitro posttranslationally by phosphorylation/dephosphorylation
Publisher
An entity responsible for making the resource available
Hepatology
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-12
Subject
The topic of the resource
bile-acid synthesis; cloning; dietary-cholesterol; enzyme; Escherichia coli; expression; Gastroenterology & Hepatology; hmg-coa reductase; messenger-rna levels; Phosphatase; purification
Creator
An entity primarily responsible for making the resource
Nguyen L B; Shefer S; Salen G; Chiang J Y L; Patel M
Description
An account of the resource
Purified cholesterol 7 alpha-hydroxylases (C7 alpha H) from human and rat liver microsomes, and from transformed Escherichia coli expression systems, were incubated with 0.3 mmol/L [gamma-P-32] adenosine triphosphate (ATP) in the presence and absence of bacterial alkaline phosphatase (AP) or rabbit muscle adenosine 3',5'-cyclic monophosphate (cAMP) dependent protein kinase. The amounts of P-32 incorporation after separation of human and rat C7 alpha H proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were related to C7 alpha H catalytic activities (determined by a radioisotope incorporation method) and enzyme protein mass (determined by Western blotting and laser densitometry). Both human and rat C7 alpha H activities significantly decreased after dephosphorylation by AP (-57%--72%) and increased up to twofold with phosphorylation by rabbit muscle cAMP-dependent protein kinase. The increases in C7 alpha H activities were proportional to the amounts of cAMP-dependent protein kinase used, and were coupled to P-32 incorporation into the purified enzymes, Both the activation of C7 alpha H and the amounts of P-32 incorporation were time-dependent and reached a maximum after 1 hour of incubation with 5 U of cAMP-dependent protein kinase. In a second set of experiments, purified human and rat Liver C7 alpha H were dephosphorylated by 30-minute incubation with AP, followed by inactivation of the phosphatase by the inhibitor NaF, and rephosphorylation of C7 alpha H by 30-minute incubation with rabbit muscle cAMP-dependent protein kinase or bovine heart cAMP-independent protein kinase. Rephosphorylation of the dephosphorylated C7 alpha H proteins by cAMP-dependent protein kinase increased C7 alpha H catalytic activities up to fourfold, and the stimulation in catalytic activities paralleled the increases in P-32 incorporation into the purified enzymes. Bovine heart protein kinase was as potent as rabbit muscle cAMP-dependent protein kinase in stimulating catalytic activity and P-32 incorporation into the human C7 alpha H protein. Because the protein mass of these purified enzymes did not change, the short-term regulation or catalytic efficiency of C7 alpha H (activity per protein mass unit) is modulated, in vitro, posttranslationally by a phosphorylation/ dephosphorylation mechanism in both the human and the rat enzymes.
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<a href="http://doi.org/10.1053/jhep.1996.v24.pm0008938182" target="_blank" rel="noreferrer noopener">10.1053/jhep.1996.v24.pm0008938182</a>
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Journal Article
1996
bile-acid synthesis
Chiang J Y L
Cloning
Department of Internal Medicine
dietary-cholesterol
enzyme
Escherichia coli
expression
Gastroenterology & Hepatology
Hepatology
HMG-CoA reductase
Journal Article
messenger-rna levels
NEOMED College of Medicine
Nguyen L B
Patel M
Phosphatase
purification
Salen G
Shefer S