1
40
4
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Hyperlink
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URL
https://doi.org/10.26502/josm.511500060
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A Pre-clinical Standard Operating Procedure for Evaluating Orthobiologics in an In Vivo Rat Spinal Fusion Model
Creator
An entity primarily responsible for making the resource
Andrew L Alejo
Scott McDermott
Yusuf Khalil
Hope C Ball
Gabrielle T Robinson
Ernesto Solorzano
Amanda M Alejo
Jacob Douglas
Trinity K Samson
Jesse W Young
Fayez F Safadi
Date
A point or period of time associated with an event in the lifecycle of the resource
2022
Description
An account of the resource
The rat animal model is a cost effective and reliable model used in spinal pre-clinical research. Complications from various surgical procedures in humans often arise that were based on these pre-clinical animal models. Therefore safe and efficacious pre-clinical animal models are needed to establish continuity into clinical trials. A Standard Operating Procedure (SOP) is a validated method that allows researchers to safely and carefully replicate previously successful surgical techniques. Thus, the aim of this study is to describe in detail the procedures involved in a common rat bilateral posterolateral intertransverse spinal fusion SOP used to test the efficacy and safety different orthobiologics using a collagen-soaked sponge as an orthobiologic carrier. Only two orthobiologics are currently FDA approved for spinal fusion surgery which include recombinant bone morphogenetic protein 2 (rhBMP-2), and I-FACTOR. While there are many additional orthobiologics currently being tested, one way to show their safety profile and gain FDA approval, is to use well established pre-clinical animal models. A preoperative, intraoperative, and postoperative surgical setup including specific anesthesia and euthanasia protocols are outlined. Furthermore, we describe different postoperative methods used to validate the spinal fusion SOP, which include μCT analysis, histopathology, biomechanical testing, and blood analysis. This SOP can help increase validity, transparency, efficacy, and reproducibly in future rat spinal fusion surgery procedures.
Source
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J Orthop Sports Med
. 2022;4(3):224-240. doi: 10.26502/josm.511500060. Epub 2022 Sep 5.
Language
A language of the resource
English
2022
Orthobiologics
Posterolateral intertransverse fusion model
Protocols
rat model
rhBMP-2
Spinal Fusion
Standard Operating Procedure (SOP)
-
Text
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URL Address
<a href="http://doi.org/10.1161/atvbaha.109.186189" target="_blank" rel="noreferrer noopener">http://doi.org/10.1161/atvbaha.109.186189</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
802-U56
Issue
6
Volume
29
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Amplification of Coronary Arteriogenic Capacity of Multipotent Stromal Cells by Epidermal Growth Factor
Publisher
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Arteriosclerosis Thrombosis and Vascular Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-06
Subject
The topic of the resource
coronary circulation; angiogenesis; collateral circulation; Cardiovascular System & Cardiology; Myocardial infarction; expression; binding; Hematology; mesenchymal stem-cells; smooth-muscle-cells; rat model; endothelial-cells; collateral growth; improve heart function
Creator
An entity primarily responsible for making the resource
Belmadani S; Matrougui K; Kolz C; Pung Y F; Palen D; Prockop D J; Chilian W M
Description
An account of the resource
Objective-We determined whether increasing adherence of multipotent stromal cells (MSCs) would amplify their effects on coronary collateral growth (CCG). Methods and Results-Adhesion was established in cultured coronary endothelials cells (CECs) or MSCs treated with epidermal growth factor (EGF). EGF increased MSCs adhesion to CECs, and increased intercellular adhesion molecule (ICAM-1) or vascular cell adhesion molecule (VCAM-1) expression. Increased adherence was blocked by EGF receptor antagonism or antibodies to the adhesion molecules. To determine whether adherent MSCs, treated with EGF, would augment CCG, repetitive episodes of myocardial ischemia (RI) were introduced and CCG was measured from the ratio of collateral-dependent (CZ) and normal zone (NZ) flows. CZ/NZ was increased by MSCs without treatment versus RI-control and was further increased by EGF-treated MSCs. EGF-treated MSCs significantly improved myocardial function versus RI or RI + MSCs demonstrating that the increase in collateral flow was functionally significant. Engraftment of MSCs into myocardium was also increased by EGF treatment. Conclusions-These results reveal the importance of EGF in MSCs adhesion to endothelium and suggest that MSCs may be effective therapies for the stimulation of coronary collateral growth when interventions are used to increase their adhesion and homing (in vitro EGF treatment) to the jeopardized myocardium. (Arterioscler Thromb Vasc Biol. 2009; 29: 802-808.)
Identifier
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<a href="http://doi.org/10.1161/atvbaha.109.186189" target="_blank" rel="noreferrer noopener">10.1161/atvbaha.109.186189</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2009
angiogenesis
Arteriosclerosis Thrombosis and Vascular Biology
Belmadani S
Binding
Cardiovascular System & Cardiology
Chilian W M
Collateral Circulation
collateral growth
Coronary Circulation
endothelial-cells
expression
Hematology
improve heart function
Journal Article or Conference Abstract Publication
Kolz C
Matrougui K
mesenchymal stem-cells
myocardial infarction
Palen D
Prockop D J
Pung Y F
rat model
smooth-muscle-cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1371/journal.pone.0038359" target="_blank" rel="noreferrer noopener">http://doi.org/10.1371/journal.pone.0038359</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
13-13
Issue
5
Volume
7
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Performance of Repetitive Tasks Induces Decreased Grip Strength and Increased Fibrogenic Proteins in Skeletal Muscle: Role of Force and Inflammation
Publisher
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PLOS ONE
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-05
Subject
The topic of the resource
carpal-tunnel-syndrome; factor gene-expression; flexor tendon cells; in-vivo; lengthening contractions; necrosis-factor-alpha; rat model; Science & Technology - Other Topics; strain injury; tissue growth-factor; tnf-alpha
Creator
An entity primarily responsible for making the resource
Abdelmagid S M; Barr A E; Rico M; Amin M; Litvin J; Popoff S N; Safadi F; Barbe M F
Description
An account of the resource
Background: This study elucidates exposure-response relationships between performance of repetitive tasks, grip strength declines, and fibrogenic-related protein changes in muscles, and their link to inflammation. Specifically, we examined forearm flexor digitorum muscles for changes in connective tissue growth factor (CTGF; a matrix protein associated with fibrosis), collagen type I (Col1; a matrix component), and transforming growth factor beta 1 (TGFB1; an upstream modulator of CTGF and collagen), in rats performing one of two repetitive tasks, with or without anti-inflammatory drugs. Methodology/Results: To examine the roles of force versus repetition, rats performed either a high repetition negligible force food retrieval task (HRNF), or a high repetition high force handle-pulling task (HRHF), for up to 9 weeks, with results compared to trained only (TR-NF or TR-HF) and normal control rats. Grip strength declined with both tasks, with the greatest declines in 9-week HRHF rats. Quantitative PCR (qPCR) analyses of HRNF muscles showed increased expression of Col1 in weeks 3-9, and CTGF in weeks 6 and 9. Immunohistochemistry confirmed PCR results, and also showed greater increases of CTGF and collagen matrix in 9-week HRHF rats than 9-week HRNF rats. ELISA, and immunohistochemistry revealed greater increases of TGFB1 in TR-HF and 6-week HRHF, compared to 6-week HRNF rats. To examine the role of inflammation, results from 6-week HRHF rats were compared to rats receiving ibuprofen or anti-TNF-alpha treatment in HRHF weeks 4-6. Both treatments attenuated HRHF-induced increases in CTGF and fibrosis by 6 weeks of task performance. Ibuprofen attenuated TGFB1 increases and grip strength declines, matching our prior results with anti-TNF alpha. Conclusions/Significance: Performance of highly repetitive tasks was associated with force-dependent declines in grip strength and increased fibrogenic-related proteins in flexor digitorum muscles. These changes were attenuated, at least short-term, by anti-inflammatory treatments.
Identifier
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<a href="http://doi.org/10.1371/journal.pone.0038359" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0038359</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2012
Abdelmagid S M
Amin M
Barbe M F
Barr A E
carpal-tunnel-syndrome
factor gene-expression
flexor tendon cells
in-vivo
Journal Article or Conference Abstract Publication
lengthening contractions
Litvin J
necrosis-factor-alpha
PloS one
Popoff S N
rat model
Rico M
Safadi F
Science & Technology - Other Topics
strain injury
tissue growth-factor
TNF-alpha
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.nurt.2008.10.035" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.nurt.2008.10.035</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
141-151
Issue
1
Volume
6
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Dual-Target-Directed Drugs that Block Monoamine Oxidase B and Adenosine A(2A) Receptors for Parkinson's Disease
Publisher
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Neurotherapeutics
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-01
Subject
The topic of the resource
adenosine A(2A) receptor; antagonist; caffeine; dopaminergic neurotoxicity; dual-target-directed drug; human-brain; inhibition; istradefylline; l-dopa; mao-b; monoamine-oxidase-b; motor complications; Neurosciences & Neurology; parkinsons-disease; Pharmacology & Pharmacy; rat model; substantia-nigra
Creator
An entity primarily responsible for making the resource
Petzer J P; Castagnoli N; Schwarzschild M A; Chen J F; Van der Schyf C J
Description
An account of the resource
Inadequacies of the current pharmacotherapies to treat Parkinson's disease (PD) have prompted efforts to identify novel drug targets. The adenosine A(2A) receptor is one such target. Antagonists of this receptor (A(2A) antagonists) are considered promising agents for the symptomatic treatment of PD. Evidence suggests that A(2A) antagonists may also have neuroprotective properties that may prevent the development of the dyskinesia that often complicates levodopa treatment. Because the therapeutic benefits of A(2A) antagonists are additive to that of dopamine replacement therapy, it may be possible to reduce the dose of the dopaminergic drugs and therefore the occurrence of side effects. Inhibitors of monoamine oxidase (MAO)-B also are considered useful tools for the treatment of PD. When used in combination with levodopa, inhibitors of MAO-B may enhance the elevation of dopamine levels after levodopa treatment, particularly when used in early stages of the disease when dopamine production may not be so severely compromised. Furthermore, MAO-B inhibitors may also possess neuroprotective properties in part by reducing the damaging effect of dopamine turnover in the brain. These effects of MAO-B inhibitors are especially relevant when considering that the brain shows an age-related increase in MAO-B activity. Based on these observations, dual-target-directed drugs, compounds that inhibit MAO-B and antagonize A(2A) receptors, may have value in the management of PD. This review summarizes recent efforts to develop such dual-acting drugs using caffeine as the lead compound.
Identifier
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<a href="http://doi.org/10.1016/j.nurt.2008.10.035" target="_blank" rel="noreferrer noopener">10.1016/j.nurt.2008.10.035</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2009
adenosine A(2A) receptor
Antagonist
caffeine
Castagnoli N
Chen J F
dopaminergic neurotoxicity
dual-target-directed drug
human-brain
inhibition
istradefylline
Journal Article
l-dopa
mao-b
monoamine-oxidase-b
motor complications
Neurosciences & Neurology
Neurotherapeutics
parkinsons-disease
Petzer J P
Pharmacology & Pharmacy
rat model
Schwarzschild M A
substantia-nigra
Van der Schyf C J