1
40
3
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1089/ten.2006.12.691" target="_blank" rel="noreferrer noopener">http://doi.org/10.1089/ten.2006.12.691</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
691-703
Issue
4
Volume
12
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Comparison Of Different Chondrocytes For Use In Tissue Engineering Of Cartilage Model Structures
Publisher
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Tissue Engineering
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-04
Subject
The topic of the resource
articular-cartilage; auricular cartilage; Cell Biology; construct; growth; in-vitro; regeneration; scaffold; shape; stem-cells; vivo
Creator
An entity primarily responsible for making the resource
Isogai N; Kusuhara H; Ikada Y; Ohtani H; Jacquet R; Hillyer J; Lowder E; Landis W J
Description
An account of the resource
This study compares bovine chondrocytes harvested from four different animal locations-nasoseptal, articular, costal, and auricular-for tissue-engineered cartilage modeling. While the work serves as a preliminary investigation for fabricating a human ear model, the results are important to tissue-engineered cartilage in general. Chondrocytes were cultured and examined to determine relative cell proliferation rates, type II collagen and aggrecan gene expression, and extracellular matrix production. Respective chondrocytes were then seeded onto biodegradable poly(L-lactide-epsilon-caprolactone) disc-shaped scaffolds. Cell-copolymer constructs were cultured and subsequently implanted in the subcutaneous space of athymic mice for up to 20 weeks. Neocartilage development in harvested constructs was assessed by molecular and histological means. Cell culture followed over periods of up to 4 weeks showed chondrocyte proliferation from the tissue sources varied, as did levels of type II collagen and aggrecan gene expression. For both genes, highest expression was found for costal chondrocytes, followed by nasoseptal, articular, and auricular cells. Retrieval of 20-week discs from mice revealed changes in construct dimensions with different chondrocytes. Greatest disc diameter was found for scaffolds seeded with auricular chondrocytes, followed by those with costal, nasoseptal, and articular cells. Greatest disc thickness was measured for scaffolds containing costal chondrocytes, followed by those with nasoseptal, auricular, and articular cells. Retrieved copolymer alone was smallest in diameter and thickness. Only auricular scaffolds developed elastic fibers after 20 weeks of implantation. Type II collagen and aggrecan were detected with differing expression levels on quantitative RT-PCR of discs implanted for 20 weeks. These data demonstrate that bovine chondrocytes obtained from different cartilaginous sites in an animal may elicit distinct responses during their respective development of a tissue-engineered neocartilage. Thus, each chondrocyte type establishes or maintains its particular developmental characteristics, and this observation is critical in the design and elaboration of any tissue-engineered cartilage model.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1089/ten.2006.12.691" target="_blank" rel="noreferrer noopener">10.1089/ten.2006.12.691</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2006
articular-cartilage
auricular cartilage
Cell Biology
construct
growth
Hillyer J
Ikada Y
in-vitro
Isogai N
Jacquet R
Journal Article or Conference Abstract Publication
Kusuhara H
Landis W J
Lowder E
Ohtani H
Regeneration
scaffold
shape
stem-cells
Tissue Engineering
vivo
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/ar.23064" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/ar.23064</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
328-333
Issue
1
Volume
298
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Dublin Core
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Title
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Morphometry, Geometry, Function, and the Future
Publisher
An entity responsible for making the resource available
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-01
Subject
The topic of the resource
Anatomy & Morphology; evolution; morphology; performance; primates; darwins finches; adaptation; selection; form; evolutionary; fitness; shape; biological anthropology; geometric morphometrics
Creator
An entity primarily responsible for making the resource
McNulty K P; Vinyard C J
Description
An account of the resource
The proliferation of geometric morphometrics (GM) in biological anthropology and more broadly throughout the biological sciences has resulted in a multitude of studies that adopt landmark-based approaches for addressing a variety of questions in evolutionary morphology. In some cases, particularly in the realm of systematics, the fit between research question and analytical design is quite good. Functional-adaptive studies, however, do not readily conform to the methods available in the GM toolkit. The symposium organized by Terhune and Cooke entitled Assessing function via shape: What is the place of GM in functional morphology? held at the 2013 meetings of the American Association of Physical Anthropologists was designed specifically to explore this relationship between landmark-based methods and analyses of functional morphology, and the articles in this special issue, which stem in large part from this symposium, provide numerous examples of how the two approaches can complement and contrast each other. Here, we underscore some of the major difficulties in interpreting GM results within a functional regime. In combination with other contributions in this issue, we identify emerging areas of research that will help bridge the gap between multivariate morphometry and functional-adaptive analysis. Ultimately, neither geometric nor functional morphometric approaches is sufficient to elaborate the adaptive pathways that explain morphological evolution through natural selection. These perspectives must be further integrated with research from physiology, developmental biology, genomics, and ecology. Anat Rec, 298:328-333, 2015. (c) 2014 Wiley Periodicals, Inc.
Identifier
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<a href="http://doi.org/10.1002/ar.23064" target="_blank" rel="noreferrer noopener">10.1002/ar.23064</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2015
Adaptation
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
Anatomy & Morphology
biological anthropology
darwins finches
Evolution
evolutionary
fitness
form
geometric morphometrics
Journal Article or Conference Abstract Publication
McNulty K P
morphology
Performance
Primates
Selection
shape
Vinyard C J
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bpj.2010.04.011" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bpj.2010.04.011</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
115-123
Issue
1
Volume
99
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Dublin Core
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Title
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Actomyosin Tension Exerted on the Nucleus through Nesprin-1 Connections Influences Endothelial Cell Adhesion, Migration, and Cyclic Strain-Induced Reorientation
Publisher
An entity responsible for making the resource available
Biophysical Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-07
Subject
The topic of the resource
cytoskeleton; Biophysics; in-vitro; actin; envelope; polarization; dreifuss muscular-dystrophy; focal adhesions; lamin-a/c; mechanical stiffness; membrane protein; shape
Creator
An entity primarily responsible for making the resource
Chancellor T J; Lee J; Thodeti C K; Lele T
Description
An account of the resource
Endothelial cell polarization and directional migration is required for angiogenesis. Polarization and motility requires not only local cytoskeletal remodeling but also the motion of intracellular organelles such as the nucleus. However, the physiological significance of nuclear positioning in the endothelial cell has remained largely unexplored. Here, we show that siRNA knockdown of nesprin-1, a protein present in the linker of nucleus to cytoskeleton complex, abolished the reorientation of endothelial cells in response to cyclic strain. Confocal imaging revealed that the nuclear height is substantially increased in nesprin-1 depleted cells, similar to myosin inhibited cells. Nesprin-1 depletion increased the number of focal adhesions and substrate traction while decreasing the speed of cell migration; however, there was no detectable change in nonmuscle myosin II activity in nesprin-1 deficient cells. Together, these results are consistent with a model in which the nucleus balances a portion of the actomyosin tension in the cell. In the absence of nesprin-1, actomyosin tension is balanced by the substrate, leading to abnormal adhesion, migration, and cyclic strain-induced reorientation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.bpj.2010.04.011" target="_blank" rel="noreferrer noopener">10.1016/j.bpj.2010.04.011</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2010
actin
Biophysical Journal
Biophysics
Chancellor T J
cytoskeleton
dreifuss muscular-dystrophy
envelope
Focal Adhesions
in-vitro
Journal Article or Conference Abstract Publication
lamin-a/c
Lee J
Lele T
mechanical stiffness
membrane protein
polarization
shape
Thodeti C K