Estrogen And Parkinson's Disease
alpha-synuclein gene; antiphospholipid antibodies; brain dopamine; dopamine; female rats; gender differences; nigrostriatal dopaminergic system; oral-contraceptives; postmenopausal women; sex-differences; striatal
Horstink Mwim; Strijks E; Dluzen D E
Parkinson's Disease
2003
2003
Book Chapter
n/a
Inhibition Of Striatal Dopamine Transporter Activity By 17 Beta-estradiol
brain; dopamine transporter; estradiol; estrous-cycle; gonadal steroid hormone; h-3 gbr-12935 binding; mptp-induced neurotoxicity; nigrostriatal; nucleus-accumbens; Pharmacology & Pharmacy; progesterone; protein-kinase-c; release; sex-differences; striatal; synaptosome; uptake inhibition; uptake sites
Disshon K A; Boja J W; Dluzen D E
European Journal of Pharmacology
1998
1998-03
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/s0014-2999(98)00008-9" target="_blank" rel="noreferrer noopener">10.1016/s0014-2999(98)00008-9</a>
Multiple binding sites for I-125 RTI-121 and other cocaine analogs in rat frontal cerebral cortex
Neurosciences & Neurology; localization; h-3 gbr-12935 binding; transporter; membranes; dopamine transporter; cocaine; nucleus-accumbens; norepinephrine transporter; medial prefrontal cortex; brain synaptosomes; glycosylation; human dopamine; reinforcing properties; RTI-121; self-stimulation; serotonin transporter; striatal
In an effort to identify novel binding sites for cocaine and its analogs, we carried out binding studies with the high-affinity and selective ligand [I-125]RTI-121 in rat frontal cortical tissue. Very low densities of binding sites were found. Saturation analysis revealed that the binding was to both high- and low-affinity sites. Pharmacological competition studies were carried out with inhibitors of the dopamine, norepinephrine, and serotonin transporters. The various transporter inhibitors inhibited the binding of 15 pM [I-125]RTI-121 in a biphasic fashion following a two-site binding model. The resultant data were complex and did not suggest a simple association with any single transporter. Correlational analysis supported the following hypothesis: [I-125] RTI-121 binds to known transporters and not to novel sites; these include dopamine, norepinephrine, and serotonin transporters. Immunoprecipitation of transporters photoaffinity labeled with [(125)] RTI-82 and subsequent analysis of SDS-page gels revealed the presence of authentic dopamine transporters in these samples; displacement of the photoaffinity label occurred with a typical dopamine transporter pharmacology. These data are compatible with the binding properties of RTI-181 and the presence of several known transporters in the tissue studied. Synapse 30:9-17, 1998. (C) 1998 Wiley-Liss, Inc.dagger
Boja J W; Carroll F I; Vaughan R A; Kopajtic T; Kuhar M J
Synapse
1998
1998-09
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1002/(sici)1098-2396(199809)30:1%3C9::aid-syn2%3E3.3.co;2-p" target="_blank" rel="noreferrer noopener">10.1002/(sici)1098-2396(199809)30:1%3C9::aid-syn2%3E3.3.co;2-p</a>