Changes In Spinal Serotonin Turnover Mediate Age-related Differences In The Behavioral Manifestations Of Peripheral Nerve Injury
allodynia; Behavioral Sciences; brain; endorphin; hyperalgesia; monoamine oxidase; mononeuropathy; neuropathic pain; Neurosciences & Neurology; norepinephrine; oxidative stress; Pharmacology & Pharmacy; rat; sciatic nerve; serotonin; spinal cord; systems
Lovell J A; Novak J C; Stuesse S L; Cruce W L R; Crisp T
Pharmacology Biochemistry and Behavior
2000
2000-08
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/s0091-3057(00)00285-9" target="_blank" rel="noreferrer noopener">10.1016/s0091-3057(00)00285-9</a>
Recognition And Understanding Of Goals And Roles: The Key Internal Features Of Mental Health Court Teams
boundaries; community treatment; conflict; Government & Law; ill offenders; Interdisciplinary team; justice; law-enforcement; Mental health court; Mental illness; Professional roles; Psychiatry; systems; working
Gallagher M; Skubby D; Bonfine N; Munetz M R; Teller J L S
International Journal of Law and Psychiatry
2011
2011-11
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/j.ijlp.2011.10.005" target="_blank" rel="noreferrer noopener">10.1016/j.ijlp.2011.10.005</a>
Cholesterol-Peptide Hybrids to Form Liposome-Like Vesicles for Gene Delivery
condensation; dna; histidine; nanoparticles; oligopeptides; polymers; prospects; Science & Technology - Other Topics; systems; therapy; vectors
In this paper, four amphiphilic cholesterol-peptide conjugates (Ch-R5H5, Ch-R3H3, Ch-R5 and Ch-R5) were designed and synthesized, and their properties in gene delivery were evaluated in vitro with an aim of developing more efficient gene delivery carriers. These amphiphilic cholesterol-peptide conjugates are composed of hydrophobic cholesterol and positively charged peptides. They were able to self-assemble into micelles at low concentrations and their critical micelle concentrations in phosphate buffered saline (pH 7.4) are <= 85 mu g/mL. Amphiphilic cholesterol-peptide conjugates condensed DNA more efficiently than a hydrophilic cationic oligoarginine (R10) peptide with no hydrophobic segment. Their transfection efficiencies were at least two orders of magnitude greater than that of R10 peptide in HEK-293 cells. Moreover, the introduction of histidine residues in cholesterol-peptide conjugates led to higher gene expression efficiency compared with cholesterol-peptides without histidine (Ch-R5 and Ch-R3), and the luciferase expression level was comparable or even higher than that induced by PEI at its optimal N/P ratio. In particular, Ch-R5H5 condensed DNA into smaller nanoparticles than Ch-R3H3 at higher N/P ratios, and the minimum size of Ch-R5H5/DNA complexes was 180 nm with zeta potential of 23 mV, achieved at the N/P ratio of 30. This liposome-like vesicle may be a promising gene delivery carrier for intravenous therapy.
Tang Q; Cao B; Wu H Y; Cheng G
PLOS ONE
2013
2013-01
Journal Article
<a href="http://doi.org/10.1371/journal.pone.0054460" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0054460</a>
Consensus Development of a Modern Ontology of Emergency Department Presenting Problems-The Hierarchical Presenting Problem Ontology (HaPPy)
care; chief complaint; coded chief complaints; electronic medical-record; emergency; emergency department; Medical Informatics; medicine; ontology; presenting problem; rods; systems; terminologies
Objective Numerous attempts have been made to create a standardized "presenting problem" or "chief complaint" list to characterize the nature of an emergency department visit. Previous attempts have failed to gain widespread adoption as they were not freely shareable or did not contain the right level of specificity, structure, and clinical relevance to gain acceptance by the larger emergency medicine community. Using real-world data, we constructed a presenting problem list that addresses these challenges. Materials and Methods We prospectively captured the presenting problems for 180,424 consecutive emergency department patient visits at an urban, academic, Level I trauma center in the Boston metro area. No patients were excluded. We used a consensus process to iteratively derive our system using real-world data. We used the first 70% of consecutive visits to derive our ontology, followed by a 6-month washout period, and the remaining 30% for validation. All concepts were mapped to Systematized Nomenclature of Medicine-Clinical Terms (SNOMED CT). Results Our system consists of a polyhierarchical ontology containing 692 unique concepts, 2,118 synonyms, and 30,613 nonvisible descriptions to correct misspellings and nonstandard terminology. Our ontology successfully captured structured data for 95.9% of visits in our validation data set. Discussion and Conclusion We present the HierArchical Presenting Problem ontologY (HaPPy). This ontology was empirically derived and then iteratively validated by an expert consensus panel. HaPPy contains 692 presenting problem concepts, each concept being mapped to SNOMED CT. This freely sharable ontology can help to facilitate presenting problem-based quality metrics, research, and patient care.
Horng S; Greenbaum N R; Nathanson L A; McClay J C; Goss F R; Nielson J A
Applied Clinical Informatics
2019
2019-05
<a href="https://www.biorxiv.org/content/10.1101/126870v2" target="_blank" rel="noreferrer noopener">10.1055/s-0039-1691842</a>