1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1021/bi9018237" target="_blank" rel="noreferrer noopener">http://doi.org/10.1021/bi9018237</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 2529-2539 Issue 11 Volume 49 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Peroxynitrite-Mediated Oxidative Modifications of Complex II: Relevance in Myocardial Infarction Publisher An entity responsible for making the resource available Biochemistry Date A point or period of time associated with an event in the lifecycle of the resource 2010 2010-03 Subject The topic of the resource Biochemistry & Molecular Biology; cytochrome-c; fatty-acids; identification; membrane; mitochondrial; modulation; nitric-oxide; postischemic heart; succinate-ubiquinone; thiyl Creator An entity primarily responsible for making the resource Zhang L W; Chen C L; Kang P T; Garg V; Hu K L; Green-Church K B; Chen Y R Description An account of the resource Increased O-2(center dot-) and NO production is a key mechanism of mitochondrial dysfunction in mycocardial ischemia/reperfusion injury. In complex impairment and enhanced tyrosine nitration of the 70 kDa FAD-binding protein occur in the post-ischemic myocardium and are thought to be mediated by peroxynitrite (OONO-) ill vivo [Chen, Y.-R., et ill. (2008) J. Biol. Chem. 283, 27991-28003]. To gain deeper insights into the redox protein thiols involved ill OONO--mediated Oxidative post-translational modifications relevant ill myocardial infarction, We subjected Isolated myocardial complex 11 to ill vltro protein nitration with OONO-. This resulted ill site-specific nitration at the 70 kDa polypeptide and impairment of complex II-derived electron transfer activity. Under reducing conditions, the gel band of the 70 kDa POlyPePtldC MIS subjected to in-gel trypsin/chymotrypsin digestion and then LC-MS/MS analysis Nitration Of Y-56 and Y-142 was previosly reported. Further analysis revealed that C-267, C-476 and C-537 are involved ill OONO--mediated S-sulfonation. To identify the disulfide formation mediated by OONO-, nitrated complex 11 was alkylated with iodoacetamide. In-gel proteolytic digestion and LC-MS/MS analysis were conducted under nonreducing conditions. The MS/MS data were examined with MassMatrix, indicating that three cysteine pairs, C-306-C-312,C-439-C-444, and C-288-C-575, were involved in OONO--mediated disulfide formation. Immuno-spin trapping with an anti-DMPO antibody and subsequent MS Was used to define Oxidative modification with protein radical formation. An OONO--dependent DMPO adduct was detected. and further LC-MS/MS analysis indicated C-288 and C-655 were involved in DMPO binding. These I-CSLIltS offered a complete profile of OONO--mediated Oxidative modifications that may be relevant Ill the disease model of myocardial infarction. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1021/bi9018237" target="_blank" rel="noreferrer noopener">10.1021/bi9018237</a> Format The file format, physical medium, or dimensions of the resource Journal Article 2010 BIOCHEMISTRY Biochemistry & Molecular Biology Chen C L Chen Y R cytochrome-c fatty-acids Garg V Green-Church K B Hu K L identification Journal Article Kang P T membrane Mitochondrial modulation nitric-oxide postischemic heart succinate-ubiquinone thiyl Zhang L W