1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M110.134890" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M110.134890</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
32182-32191
Issue
42
Volume
285
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Ampk-dependent Repression Of Hepatic Gluconeogenesis Via Disruption Of Creb Center Dot Crtc2 Complex By Orphan Nuclear Receptor Small Heterodimer Partner
Publisher
An entity responsible for making the resource available
Journal of Biological Chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-10
Subject
The topic of the resource
activated protein-kinase; binding-protein; Biochemistry & Molecular Biology; creb coactivator crtc2; gene-expression; insulin; metformin; phosphorylation; shp; torc2; transcriptional activity
Creator
An entity primarily responsible for making the resource
Lee J M; Seo W Y; Song K H; Chanda D; Kim Y D; Kim D K; Lee M W; Ryu D; Kim Y H; Noh J R; Lee C H; Chiang J Y L; Koo S H; Choi H S
Description
An account of the resource
Orphan nuclear receptor small heterodimer partner (SHP) plays a key role in transcriptional repression of gluconeogenic enzyme gene expression. Here, we show that SHP inhibited protein kinase A-mediated transcriptional activity of cAMP-response element-binding protein (CREB), a major regulator of glucose metabolism, to modulate hepatic gluconeogenic gene expression. Deletion analysis of phosphoenolpyruvate carboxykinase (PEPCK) promoter demonstrated that SHP inhibited forskolin-mediated induction of PEPCK gene transcription via inhibition of CREB transcriptional activity. In vivo imaging demonstrated that SHP inhibited CREB-regulated transcription coactivator 2 (CRTC2)-mediated cAMP-response element-driven promoter activity. Furthermore, overexpression of SHP using adenovirus SHP decreased CRTC2-dependent elevations in blood glucose levels and PEPCK or glucose-6-phosphatase (G6Pase) expression in mice. SHP and CREB physically interacted and were co-localized in vivo. Importantly, SHP inhibited both wild type CRTC2 and S171A (constitutively active form of CRTC2) coactivator activity and disrupted CRTC2 recruitment on the PEPCK gene promoter. In addition, metformin or overexpression of a constitutively active form of AMPK (Ad-CA-AMPK) inhibited S171A-mediated PEPCK and G6Pase gene expression, and hepatic glucose production and knockdown of SHP partially relieved the metformin- and Ad-CA-AMPK-mediated repression of hepatic gluconeogenic enzyme gene expression in primary rat hepatocytes. In conclusion, our results suggest that a delayed effect of metformin-mediated induction of SHP gene expression inhibits CREB-dependent hepatic gluconeogenesis.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M110.134890" target="_blank" rel="noreferrer noopener">10.1074/jbc.M110.134890</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2010
activated protein-kinase
binding-protein
Biochemistry & Molecular Biology
Chanda D
Chiang J Y L
Choi H S
creb coactivator crtc2
gene-expression
insulin
Journal Article or Conference Abstract Publication
Journal of Biological Chemistry
Kim D K
Kim Y D
Kim Y H
Koo S H
Lee C H
Lee J M
Lee M W
metformin
Noh J R
Phosphorylation
Ryu D
Seo W Y
SHP
Song K H
torc2
transcriptional activity