Autoschizis Of Human Ovarian Carcinoma Cells: Scanning Electron And Light Microscopy Of A New Cell Death Induced By Sodium Ascorbate: Menadione Treatment
adenocarcinoma; ascorbate; autoschizis; cancer-chemotherapy; combined vitamin-c; cultured-mammalian-cells; growth-invitro; induced oxidative stress; Instruments & Instrumentation; MDAH 2774; menadione; Microscopy; Microscopy; n-nitrosomorpholine; nuclear matrix; ovary; scanning; synergistic antitumor-activity; tumor-cells; ultrastructural aspects
Gilloteaux J; Jamison J M; Arnold D; Jarjoura D; Von Greuningen V; Summers J L
Scanning
2003
2003-05
Journal Article or Conference Abstract Publication
n/a
Suppression Of The Invasive Capacity Of Human Breast Cancer Cells By Inhibition Of Urokinase Plasminogen Activator Via Amiloride And B428
angiogenesis; carcinoma; integrins; migration; prevention; prognostic marker; proliferation; pulmonary metastases; receptor; Surgery; tumor-cells
Evans D M; Sloan-Stakleff K
American Surgeon
2000
2000-05
Journal Article or Conference Abstract Publication
n/a
Identification of a Class of Novel Tubulin Inhibitors
agents; analog jcc76; biological evaluation; breast-cancer cells; halichondrin-b; heat-shock proteins; hsp27 phosphorylation; in-vivo; nimesulide; nonsteroidal antiinflammatory drugs; Pharmacology & Pharmacy; tumor-cells
We previously developed a series of anticancer agents based on cyclooxygenase-2 (COX-2) inhibitor nimesulide as a lead compound. However, the molecular targets of these agents still remain unclear. In this study, we synthesized a biotinylated probe based on a representative molecule of the compound library and performed protein pull-down assays to purify the anticancer targets of the compound. Via proteomic approaches, the major proteins bound to the probe were identified to be tubulin and heat shock protein 27 (Hsp27), and the compound inhibited tubulin polymerization by binding at the colchicine domain. However, the tubulin inhibitory effect of the compound activated the Hsp27 phosphorylation and possibly overrode the direct Hsp27 inhibitory effects, which made it difficult to solely validate the Hsp27 target. Taken together, the compound was a dual ligand of tubulin and Hsp27, inhibited tubulin polymerization, and had the potential to be a class of new chemotherapeutic agents.
Yi X; Zhong B; Smith K M; Geldenhuys W J; Feng Y; Pink J J; Dowlati A; Xu Y; Zhou A M; Su B
Journal of Medicinal Chemistry
2012
2012-04
Journal Article
<a href="http://doi.org/10.1021/jm300100d" target="_blank" rel="noreferrer noopener">10.1021/jm300100d</a>
The Art and Science of Surgical Margins for the Dermatopathologist
basal cell carcinoma; basal cell carcinoma; cutaneous melanoma; Dermatology; excision; field cells; lentigo maligna melanoma; local recurrence; margins; neurotropic melanoma; perineural invasion; primary cutaneous melanoma; prognostic-factors; skin cancer; squamous cell carcinoma; staged excision; surgical; tumor-cells
Basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and primary cutaneous melanoma (PCM) are the major forms of skin cancer. Surgical excision is one of the most frequently utilized treatment modalities for these tumors. Methods: literature review. Results: recommendations for lateral surgical excision margin (LEM) for BCCs is 4 mm for low-risk BCCs and Mohs surgery or resection with complete circumferential peripheral and deep margin assessment for high risk. Recommended LEM is 4-6 mm for low-risk SCCs and Mohs surgery or resection with complete circumferential peripheral and deep margin assessment for high risk BCCs. If SCC or BCC is >20 mm in area L with no other high-risk factors and can be repaired primarily, 10-mm clinical margins may be used. Recommended LEM is 5 mm for melanoma-in-situ; 1 cm for PCM <1 mm (Breslow); 1-2 cm for PCM 1.01-2 mm (Breslow); and, 2-3 cm for high-risk PCM >2.01 mm (Breslow). Tumor subtype-specific recommendations for histologic margins are offered which provide the greatest degree of certainty regarding the completeness of excision. Conclusion: Recommendations can be made regarding appropriate surgical excision margins by classifying skin cancers as low-risk or high-risk based on histopathological and clinical factors. Ascertaining that histopathologic margins are free of tumor is not a perfect science and requires thoughtful sampling, grossing, and staining procedures.
Weinstein M C; Brodell R T; Bordeaux J; Honda K
American Journal of Dermatopathology
2012
2012-10
Journal Article
<a href="http://doi.org/10.1097/DAD.0b013e31823347cb" target="_blank" rel="noreferrer noopener">10.1097/DAD.0b013e31823347cb</a>
The association of vitamins C and K-3 kills cancer cells mainly by autoschizis, a novel form of cell death. Basis for their potential use coadjuvants in anticancer therapy
antitumor-activity; ascorbic-acid; autoschizis; cancer; chemotherapy; dna; factor-kappa-b; fragmentation; induced apoptosis; lines; oxidative stress; Pharmacology & Pharmacy; pretreatment; tumor-cells; vitamins C and K-3
`Deficiency of alkaline and acid DNase is a hallmark in all non-necrotic cancer cells in animals and humans. These enzymes are reactivated at early stages of cancer cell death by vitamin C (acid DNase) and vitamin K-3 (alkaline DNase). Moreover, the coadministration of these vitamins (in a ratio of 100:1, for C and K-3, respectively) produced selective cancer cell death. Detailed morphological studies indicated that cell death is produced mainly by autoschizis, a new type of cancer cell death. Several mechanisms are involved in such a cell death induced by CK3, they included: formation of H2O2 during vitamins redox cycling, oxidative stress, DNA fragmentation, no caspase-3 activation, and cell membrane injury with progressive loss of organelle-free cytoplasm. Changes in the phosphorylation level of some critical proteins leading to inactivation of NF-kappaB appear as main intracellular signal transduction pathways. The increase knowledge in the mechanisms underlying cancer cells death by CK3 may ameliorate the techniques of their in vivo administration. The aim is to prepare the introduction of the association of vitamins C and K-3 into human clinics as a new, non-toxic adjuvant cancer therapy. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
Verrax J; Cadrobbi J; Delvaux M; Jamison J M; Gilloteaux J; Summers J L; Taper H S; Calderon P B
European Journal of Medicinal Chemistry
2003
2003-05
Journal Article
<a href="http://doi.org/10.1016/s0223-5234(03)00082-5" target="_blank" rel="noreferrer noopener">10.1016/s0223-5234(03)00082-5</a>