1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1016/0167-0115(91)90061-k" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0167-0115(91)90061-k</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 263-270 Issue 2 Volume 36 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource ALTERATION OF SOMATOSTATIN BUT NOT GROWTH HORMONE-RELEASING FACTOR PITUITARY BINDING-SITES IN OBESE ZUCKER RATS Publisher An entity responsible for making the resource available Regulatory Peptides Date A point or period of time associated with an event in the lifecycle of the resource 1991 1991-10 Subject The topic of the resource binding site; cells; defect; Endocrinology & Metabolism; growth hormone-releasing factor; hypothalamus; messenger-rna; Physiology; pituitary; secretion; somatostatin; tissue; zucker rat Creator An entity primarily responsible for making the resource Abribat T; Finkelstein J A; Gaudreau P Description An account of the resource The present study was designed to determine whether the diminution of growth hormone (GH) secretion that occurs in obese Zucker rats is related to alterations of GH-releasing factor (GRF) or somatostatin (SRIF) pituitary binding sites. Cold saturation studies were performed in pituitary homogenates of 4-month-old lean and obese rats, using [I-125-Tyr-10]hGRF(1-44)NH-2 as radioligand and [I-127-Tyr-10]hGRF-(1-44)NH-2 as competitor, and in pituitary membrane preparations, using [I-125-Tyr-0, D-Trp-8]SRIF14 as radioligand and [I-127-Tyr-0, D-Trp-8]SRIF14 as competitor. In lean rats, analysis of the curves by the Ligand program revealed the presence of two distinct classes of GRF binding sites, the first being of high affinity (0.74 +/- 0.11 nM) and low capacity (118 +/- 31 fmol/mg protein), the second being of lower affinity (880 +/- 240 nM) and higher capacity (140 +/- 35 pmol/mg protein), and of a single class of SRIF binding sites (affinity: 0.40 +/- 0.12 nM; capacity: 24 +/- 6 fmol/mg protein). In obese rats, no difference was observed in GRF binding parameters for both classes of sites, but the concentration of somatostatin binding sites was reduced by 67% when compared to their lean littermates. These findings suggest that the SRIF pituitary receptors are down-regulated in obese Zucker rats and indicate that no alteration of GRF pituitary binding sites contribute to the blunted GH secretion observed in this model of obesity. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1016/0167-0115(91)90061-k" target="_blank" rel="noreferrer noopener">10.1016/0167-0115(91)90061-k</a> Format The file format, physical medium, or dimensions of the resource Journal Article or Conference Abstract Publication 1991 Abribat T binding site Cells defect Endocrinology & Metabolism Finkelstein J A Gaudreau P growth hormone-releasing factor Hypothalamus Journal Article or Conference Abstract Publication messenger-rna Physiology pituitary Regulatory Peptides secretion somatostatin tissue zucker rat