Differences In Infrared Spectroscopic Data Of Connective Tissues In Transflectance And Transmittance Modes

Title

Differences In Infrared Spectroscopic Data Of Connective Tissues In Transflectance And Transmittance Modes

Creator

Hanifi A; McGoverin C; Ou Y T; Safadi F; Spencer R G; Pleshko N

Publisher

Analytica Chimica Acta

Date

2013
2013-05

Description

Fourier transform infrared imaging spectroscopy (FT-IRIS) has been used extensively to characterize the composition and orientation of macromolecules in thin tissue sections. Earlier and current studies of normal and polarized FT-IRIS data have primarily used tissues sectioned onto infrared transmissive substrates, such as salt windows. Recently, the use of low-emissivity ("low-e") substrates has become of great interest because of their low cost and favorable infrared optical properties. However, data are collected in transflectance mode when using low-e slides and in transmittance mode using salt windows. In the current study we investigated the comparability of these two modes for assessment of the composition of connective tissues. FT-IRIS data were obtained in transflectance and transmittance modes from serial sections of cartilage, bone and tendon, and from a standard polymer, polymethylmethacrylate. Both non-polarized and polarized FTIR data differed in absorbance, and in some cases peak position, between transflectance and transmittance modes. However, the FT-IRIS analysis of the collagen fibril orientation in cartilage resulted in the expected zonal arrangement of fibrils in both transmittance and transflectance. We conclude that numerical comparison of FT-IRIS-derived parameters of tissue composition should account for substrate type and data collection mode, while analysis of overall tissue architecture may be more invariant between modes. (c) 2013 Elsevier B.V. All rights reserved.

Subject

BaF2 window; bone; cartilage; Chemistry; collagen orientation; consequences; Fourier transform infrared imaging spectroscopy; Low-e; proteoglycan; scattering; slide; Transflectance; Transmittance; variance

Format

Journal Article or Conference Abstract Publication

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

41-49

Volume

779

Citation

Hanifi A; McGoverin C; Ou Y T; Safadi F; Spencer R G; Pleshko N, “Differences In Infrared Spectroscopic Data Of Connective Tissues In Transflectance And Transmittance Modes,” NEOMED Bibliography Database, accessed January 20, 2021, https://neomed.omeka.net/items/show/10070.

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