Modulation Of The Antiviral Activity Of Poly (a-u) By Ethidium Bromide And Propidium Iodide

Title

Modulation Of The Antiviral Activity Of Poly (a-u) By Ethidium Bromide And Propidium Iodide

Creator

Jamison J M; Bonilla P J; Tsai C C

Publisher

Antiviral Chemistry & Chemotherapy

Date

1990
1990-02

Description

The role of ethidium bromide (EB) and propidium iodide (PI) in modulating the antiviral and interferon-inducing activities of poly(adenylate-uridylate) (poly (A-U )) was examined using the human foreskin fibro-blast-vesicular stomatitis virus (HSF-VSV) bioassay system in which the concentration of poly (A-U) was fixed at 0.05 mM or 0.2 mM while the EB or PI concentration was varied to produce variable EB (or PI)/ribonucleotide ratios ranging from 1: 16 to 2:1. EB, PI and poly (A-U) tested individually were not efficacious antiviral agents. When poly (A-U) was combined with the ethidium bromide or propidium iodide the antiviral activity was potentiated 15- to 22-fold at EB (or Pl)/ribonucleotide ratios in the region of 1/4. The interferon-inducing activity of the EB (or Pl)/poly (A-U) combinations were equal to the sum of the interferon-inducing activity of the poly (A-U) and the EB or (PI). These results indicate that the EB and PI potentiate the antiviral activity of the poly (A-U) without superinduction of interferon. The direct viral inactivation study demonstrated that EB, PI, poly (A-U) and the EB (or Pl)/poly (A-U) combinations did not inactivate the VSV at concentrations near the 50% viral inhibitory dose.

Subject

Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Virology

Format

Journal Article or Conference Abstract Publication

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

53-60

Issue

1

Volume

1

Citation

Jamison J M; Bonilla P J; Tsai C C, “Modulation Of The Antiviral Activity Of Poly (a-u) By Ethidium Bromide And Propidium Iodide,” NEOMED Bibliography Database, accessed May 6, 2021, https://neomed.omeka.net/items/show/10313.

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