Cryopreservation Of Porcine Articular Cartilage: Mri And Biochemical Results After Different Freezing Protocols

Title

Cryopreservation Of Porcine Articular Cartilage: Mri And Biochemical Results After Different Freezing Protocols

Creator

Laouar L; Fishbein K; McGann L E; Horton W E; Spencer R G; Jomha N M

Publisher

Cryobiology

Date

2007
2007-02

Description

The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups - (1) phosphate buffered saline (PBS) control, (2) PBS snap frozen in liquid nitrogen, (3) slow-cooled in dimethyl sulfoxide (DMSO), and (4) slow cooled in PBS (in absence of DMSO). MRI results demonstrated three distinct zones in the cartilage. After exposure to ice formation during cryopreservation procedures, alterations in MRI determined matrix fixed charged density and magnetization transfer rate were noted. In addition, biochemical assays demonstrated significant alterations in chondroitin sulfate and hydroxyproline content over time without differences in hydration or DNA content. In conclusion, MRI was able to detect some changes in the intact cartilage matrix structure consistent with biochemical assessments after ice formation during cryopreservation of intact porcine AC. Furthermore, biochemical assessments supported some of these findings and changed significantly after incubating the cartilage matrix for 36-72 h in PBS in terms of chondroitin sulfate and hydroxyproline content. (c) 2006 Elsevier Inc. All rights reserved.

Subject

2-step procedure; articular-cartilage; biochemical; cryopreservation; culture cells frozen; diffusion; dimethyl sulfoxide; femoral condyles; fresh; Life Sciences & Biomedicine - Other Topics; magnetic-resonance microscopy; MRI; osteoarticular allografts; osteochondral allograft transplantation; Physiology; porcine; survival; tissue

Format

Journal Article or Conference Abstract Publication

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

36-43

Issue

1

Volume

54

Citation

Laouar L; Fishbein K; McGann L E; Horton W E; Spencer R G; Jomha N M, “Cryopreservation Of Porcine Articular Cartilage: Mri And Biochemical Results After Different Freezing Protocols,” NEOMED Bibliography Database, accessed June 23, 2021, https://neomed.omeka.net/items/show/10647.

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