Impact Of Parturition On Chemokine Homing Factor Expression In The Vaginal Distention Model Of Stress Urinary Incontinence

Title

Impact Of Parturition On Chemokine Homing Factor Expression In The Vaginal Distention Model Of Stress Urinary Incontinence

Creator

Lenis A T; Kuang M; Woo L L; Hijaz A; Penn M S; Butler R S; Rackley R; Damaser M S; Wood H M

Publisher

Journal of Urology

Date

2013
2013-04

Description

Purpose: Human childbirth simulated by vaginal distention is known to increase the expression of chemokines and receptors involved in stem cell homing and tissue repair. We hypothesized that pregnancy and parturition in rats contributes to the expression of chemokines and receptors after vaginal distention. Materials and Methods: We used 72 age matched female Lewis rats, including virgin rats with and without vaginal distention, and delivered rats with and without vaginal distention. Each rat was sacrificed immediately, or 3 or 7 days after vaginal distention and/or parturition, and the urethra was harvested. Relative expression of chemokines and receptors was determined by real-time polymerase chain reaction. Mixed models were used with the Bonferroni correction for multiple comparisons. Results: Vaginal distention up-regulated urethral expression of CCL7 immediately after injury in virgin and postpartum rats. Hypoxia inducible factor-1 alpha and vascular endothelial growth factor were up-regulated only in virgin rats immediately after vaginal distention. CD191 expression was immediately up-regulated in postpartum rats without vaginal distention compared to virgin rats without vaginal distention. CD195 was up-regulated in virgin rats 3 days after vaginal distention compared to virgin rats without vaginal distention. CD193 and CXCR4 showed delayed up-regulation in virgin rats 7 days after vaginal distention. CXCL12 was up-regulated in virgin rats 3 days after vaginal distention compared to immediately after vaginal distention. Interleukin-8 and CD192 showed no differential expression. Conclusions: Vaginal distention results in up-regulation of the chemokines and receptors expressed during tissue injury, which may facilitate the spontaneous functional recovery previously noted. Pregnancy and delivery up-regulated CD191 and attenuated the expression of hypoxia inducible factor-1 alpha and vascular endothelial growth factor in the setting of vaginal distention, likely by decreasing hypoxia.

Subject

animal-model; cells; cytokines; deficiency; delivery; female; obstetric; pregnancy; rats; simulated childbirth injuries; urethra; urogenital organs; Urology & Nephrology; vagina; women; wounds and injuries

Format

Journal Article or Conference Abstract Publication

Search for Full-text

Users with a NEOMED Library login can search for full-text journal articles at the following url: https://libraryguides.neomed.edu/home

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

1588-1594

Issue

4

Volume

189

Citation

Lenis A T; Kuang M; Woo L L; Hijaz A; Penn M S; Butler R S; Rackley R; Damaser M S; Wood H M, “Impact Of Parturition On Chemokine Homing Factor Expression In The Vaginal Distention Model Of Stress Urinary Incontinence,” NEOMED Bibliography Database, accessed April 13, 2021, https://neomed.omeka.net/items/show/10705.

Social Bookmarking