Neuropeptide Y expression defines a novel class of GABAergic projection neuron in the inferior colliculus.

Title

Neuropeptide Y expression defines a novel class of GABAergic projection neuron in the inferior colliculus.

Creator

Silveira Marina A; Anair Justin D; Beebe Nichole L; Mirjalili Pooyan; Schofield Brett R; Roberts Michael T

Publisher

The Journal of neuroscience : the official journal of the Society for Neuroscience

Date

2020
2020-05-06

Description

Located in the midbrain, the inferior colliculus (IC) integrates information from numerous auditory nuclei and is an important hub for sound processing. Despite its importance, little is known about the molecular identity and functional roles of defined neuron types in the IC. Using a multifaceted approach in mice of both sexes, we found that neuropeptide Y (NPY) expression identifies a major class of inhibitory neurons, accounting for approximately one-third of GABAergic neurons in the IC. Retrograde tracing showed that NPY neurons are principal neurons that can project to the medial geniculate nucleus. In brain slice recordings, many NPY neurons fired spontaneously, suggesting that NPY neurons may drive tonic inhibition onto postsynaptic targets. Morphological reconstructions showed that NPY neurons are stellate cells, and the dendrites of NPY neurons in the tonotopically-organized central nucleus of the IC cross isofrequency laminae. Immunostaining confirmed that NPY neurons express NPY, and we therefore hypothesized that NPY signaling regulates activity in the IC. In crosses between Npy1r(cre) and Ai14 Cre-reporter mice, we found that NPY Y1 receptor (Y1R)-expressing neurons are glutamatergic and were broadly distributed throughout the rostro-caudal extent of the IC. In whole-cell recordings, application of a high affinity Y1R agonist led to hyperpolarization in most

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Format

journalArticle

Search for Full-text

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ISSN

1529-2401 0270-6474

NEOMED College

NEOMED College of Medicine

NEOMED Department

Department of Anatomy & Neurobiology

Update Year & Number

June 2020 Update II

Citation

Silveira Marina A; Anair Justin D; Beebe Nichole L; Mirjalili Pooyan; Schofield Brett R; Roberts Michael T, “Neuropeptide Y expression defines a novel class of GABAergic projection neuron in the inferior colliculus.,” NEOMED Bibliography Database, accessed July 23, 2021, https://neomed.omeka.net/items/show/11088.

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