Small non-coding RNAome of ageing chondrocytes.

Title

Small non-coding RNAome of ageing chondrocytes.

Creator

Balaskas P; Green JA; Haqqi TM; Dyer P; Kharaz YA; Fang Y; Liu X; Welting TJM; Peffers MJ

Publisher

International Journal of Molecular Sciences

Date

2020
2020-08-07

Description

Ageing is a leading risk factor predisposing cartilage to osteoarthritis. However, little research has been conducted on the effect of ageing on the expression of small non-coding RNAs (sncRNAs). RNA from young and old chondrocytes from macroscopically normal equine metacarpophalangeal joints was extracted and subjected to small RNA sequencing (RNA-seq). Differential expression analysis was performed in R using package DESeq2. For transfer RNA (tRNA) fragment analysis, tRNA reads were aligned to horse tRNA sequences using Bowtie2 version 2.2.5. Selected microRNA (miRNAs or miRs) and small nucleolar RNA (snoRNA) findings were validated using real-time quantitative Polymerase Chain Reaction (qRT-PCR) in an extended cohort of equine chondrocytes. tRNA fragments were further investigated in low- and high-grade OA human cartilage tissue. In total, 83 sncRNAs were differentially expressed between young and old equine chondrocytes, including miRNAs, snoRNAs, small nuclear RNAs (snRNAs), and tRNAs. qRT-PCR analysis confirmed findings. tRNA fragment analysis revealed that tRNA halves (tiRNAs), tiRNA-5035-GluCTC and tiRNA-5031-GluCTC-1 were reduced in both high grade OA human cartilage and old equine chondrocytes. For the first time, we have measured the effect of ageing on the expression of sncRNAs in equine chondrocytes. Changes were detected in a number of different sncRNA species. This study supports a role for sncRNAs in ageing cartilage and their potential involvement in age-related cartilage diseases.

Subject

chondrocyte; ageing; equine; small non-coding RNA

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Format

journalArticle

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Issue

16

Volume

21

ISSN

1422-0067 1422-0067

NEOMED College

NEOMED College of Medicine

NEOMED Department

Department of Anatomy & Neurobiology
NEOMED Postdoc Publications

Update Year & Number

August 2020 List

Citation

Balaskas P; Green JA; Haqqi TM; Dyer P; Kharaz YA; Fang Y; Liu X; Welting TJM; Peffers MJ, “Small non-coding RNAome of ageing chondrocytes.,” NEOMED Bibliography Database, accessed January 27, 2021, https://neomed.omeka.net/items/show/11233.

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