Transient receptor potential vanilloid 4 channel deletion regulates pathological but not developmental retinal angiogenesis.

Title

Transient receptor potential vanilloid 4 channel deletion regulates pathological but not developmental retinal angiogenesis.

Creator

Cappelli HC;Guarino BD;Kanugula AK;Adapala R K;Perera V;Smith MA;Paruchuri S;Thodeti CK

Publisher

Journal of Cellular Physiology

Date

2020
2020-10-20

Description

Transient receptor potential vanilloid 4 (TRPV4) channels are mechanosensitive ion channels that regulate systemic endothelial cell (EC) functions such as vasodilation, permeability, and angiogenesis. TRPV4 is expressed in retinal ganglion cells, Müller glia, pigment epithelium, microvascular ECs, and modulates cell volume regulation, calcium homeostasis, and survival. TRPV4-mediated physiological or pathological retinal angiogenesis remains poorly understood. Here, we demonstrate that TRPV4 is expressed, functional, and mechanosensitive in retinal ECs. The genetic deletion of TRPV4 did not affect postnatal developmental angiogenesis but increased pathological neovascularization in response to oxygen-induced retinopathy (OIR). Retinal vessels from TRPV4 knockout mice subjected to OIR exhibited neovascular tufts that projected into the vitreous humor and displayed reduced pericyte coverage compared with wild-type mice. These results suggest that TRPV4 is a regulator of retinal angiogenesis, its deletion augments pathological retinal angiogenesis, and that TRPV4 could be a novel target for the development of therapies against neovascular ocular diseases.

Subject

neovascularization; TRPV4; mechanotransduction; ECM stiffness; human retinal endothelial cells

Identifier

Format

journalArticle

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

ISSN

1097-4652 0021-9541

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NEOMED College of Medicine
NEOMED College of Pharmacy

NEOMED Department

NEOMED Student Publications
Department of Integrative Medical Sciences
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Department of Pharmaceutical Sciences

Update Year & Number

October 2020 List

Citation

Cappelli HC;Guarino BD;Kanugula AK;Adapala R K;Perera V;Smith MA;Paruchuri S;Thodeti CK, “Transient receptor potential vanilloid 4 channel deletion regulates pathological but not developmental retinal angiogenesis.,” NEOMED Bibliography Database, accessed November 28, 2021, https://neomed.omeka.net/items/show/11340.

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