Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.

Title

Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.

Creator

Ansari MY; Haqqi TM

Publisher

Methods in Molecular Biology

Date

2021
1905-07

Description

Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines.

Subject

Inflammation; Mitochondria; Oxidative stress; Osteoarthritis; Chondrocytes; Inflammation; Osteoarthritis; Autophagy; CCCP; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Parkin; PINK1; Autophagy; CCCP; Chondrocytes; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondria; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Oxidative stress; Parkin; PINK1

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Format

journalArticle

Search for Full-text

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Pages

215-224

Volume

2245

ISSN

1940-6029 1064-3745

NEOMED College

NEOMED College of Medicine

NEOMED Department

Department of Anatomy & Neurobiology

Update Year & Number

January 2021 List

Citation

Ansari MY; Haqqi TM, “Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.,” NEOMED Bibliography Database, accessed July 7, 2022, https://neomed.omeka.net/items/show/11498.

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