A new LC-MS/MS technique for separation of gangliosides using a phenyl-hexyl column: Systematic separation according to sialic acid class and ceramide subclass
Title
A new LC-MS/MS technique for separation of gangliosides using a phenyl-hexyl column: Systematic separation according to sialic acid class and ceramide subclass
Creator
Gobburi ALP; Kipruto EW; Inman DM; Anderson DJ
Publisher
Journal Of Liquid Chromatography & Related Technologies
Date
2021
2021-01-20
Description
A LC-MS/MS technique separated the bovine and mouse brain gangliosides monosialotetrahexosylgangliosides (GM1), disialotetrahexosylgangliosides (GD1a), trisialotetrahexosylgangliosides (GT1b) and tetrasialotetrahexosylgangliosides (GQ1b) using a phenyl-hexyl HPLC column and employing a linear methanol gradient in water, which is 0.028% in ammonium hydroxide. The gangliosides were separated according to sialic acid class, and within a particular class, gangliosides having different ceramide carbon chain lengths were also separated. All gangliosides of a particular sialic acid class eluted in characteristic retention time windows in the order of GQ1b, (earliest), GT1b, GD1a, and GM1 (latest). Within each specific retention time window for a particular ganglioside class, gangliosides were separated in the order of increasing ceramide carbon chain length. The phenyl-hexyl column separation of gangliosides is advantageous over established hydrophilic interaction and conventional reversed-phase chromatography techniques, in that the former separates gangliosides according to sialic acid class but not ceramide composition and the latter distributes all the sialic acid ganglioside classes throughout the entire chromatogram. The mechanism of separation of the ganglioside sialic acid classes is proposed to be a pi-electron repulsion of negatively-charged gangliosides by the column phenyl moiety.
Subject
mass spectrometry; Gangliosides; HPLC-MS; LC-MS; MS; phenyl-hexyl column
Identifier
Rights
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Format
journalArticle
URL Address
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Pages
114-125
Issue
1-2
Volume
44
ISSN
1082-6076
NEOMED College
NEOMED College of Pharmacy
NEOMED Department
Department of Pharmaceutical Sciences
Update Year & Number
May 2021 List
Citation
Gobburi ALP; Kipruto EW; Inman DM; Anderson DJ, “A new LC-MS/MS technique for separation of gangliosides using a phenyl-hexyl column: Systematic separation according to sialic acid class and ceramide subclass,” NEOMED Bibliography Database, accessed April 23, 2024, https://neomed.omeka.net/items/show/11661.