Absence of cytochrome P450-1b1 increases susceptibility of pressure-induced axonopathy in the murine retinal projection.

Title

Absence of cytochrome P450-1b1 increases susceptibility of pressure-induced axonopathy in the murine retinal projection.

Creator

Amirmokhtari N; Foresi BD; Dewan SS; Bouhenni RA; Smith MA

Publisher

Frontiers In Cell And Developmental Biology

Date

2021
2021-03-05

Description

Adult mixed sex Cyp1b1–/– [129S6.129 × 1(B6)-Cyp1b1TM1Gonz/Mmnc] mice were obtained from the Mutant Mouse Resource and Research Center (MMRRC) at JAX (Jackson Laboratories) and genotyped before experimentation to confirm the transgene. These mice have a targeted mutation caused by a disrupted coding sequence associated with exon three of the Cyp1b1 gene. Age-matched 129S6.129 × 1(B6) mice were used as wildtype controls (Buters et al., 1999; Libby et al., 2003). Mice were maintained in the Comparative Medicine Unit at Northeast Ohio Medical University on a 12-hour light/dark cycle with standard rodent chow available ad libitum. All experimental procedures were approved by the Northeast Ohio Medical University Institutional Animal Care and Use Committee and conducted in accordance with the Guide for Care and Use of Laboratory Animals published by the National Institutes of Health.

Subject

Glaucoma is a group of heterogeneous neuro-ophthalmologic conditions that impair vision through the functional disruption and eventual degeneration of retinal ganglion cells (RGCs), the neuronal substrates responsible for eye-brain communication (Davis et al., 2016; Quigley and Broman, 2006). Glaucoma is most often attributed to the aging adult (Davis et al., 2016; Tham et al., 2014), however, befalls pediatric and adolescent populations (Kaur et al., 2011), thereby placing it in a unique group of neurodegenerative conditions that afflict populations on both ends of the lifespan.

Rights

2021 Amirmokhtari, Foresi, Dewan, Bouhenni and Smith. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Format

Journal Article

NEOMED College

NEOMED College of Medicine

NEOMED Department

Department of Pharmaceutical Sciences

Update Year & Number

jan to aug 2021 list

Citation

Amirmokhtari N; Foresi BD; Dewan SS; Bouhenni RA; Smith MA, “Absence of cytochrome P450-1b1 increases susceptibility of pressure-induced axonopathy in the murine retinal projection.,” NEOMED Bibliography Database, accessed May 25, 2024, https://neomed.omeka.net/items/show/11784.