Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.

Title

Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.

Creator

Ansari MY; Haqqi TM

Publisher

Methods in Molecular Biology

Date

2020
2020-12-15

Subject

Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines.

Rights

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Format

Journal Article

ISSN

978-1-0716-1119-7

NEOMED College

NEOMED College of Medicine

NEOMED Department

Department of Anatomy & Neurobiology

Update Year & Number

Jan to Aug list 2021

Citation

Ansari MY; Haqqi TM, “Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.,” NEOMED Bibliography Database, accessed July 7, 2022, https://neomed.omeka.net/items/show/11793.

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