Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.
Title
Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.
Creator
Ansari MY; Haqqi TM
Publisher
Methods in Molecular Biology
Date
2020
2020-12-15
Subject
Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines.
Identifier
Rights
© Springer Science+Business Media, LLC, part of Springer Nature 2021
Format
Journal Article
URL Address
ISSN
978-1-0716-1119-7
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Update Year & Number
Jan to Aug list 2021
Citation
Ansari MY; Haqqi TM, “Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.,” NEOMED Bibliography Database, accessed April 19, 2024, https://neomed.omeka.net/items/show/11793.