MicroRNA-9 promotion of interleukin-6 expression by inhibiting monocyte chemoattractant protein-induced protein 1 expression in interleukin-1beta-stimulated human chondrocytes.

MicroRNA-9 promotion of interleukin-6 expression by inhibiting monocyte chemoattractant protein-induced protein 1 expression in interleukin-1beta-stimulated human chondrocytes.

Makki Mohammad S; Haseeb Abdul; Haqqi Tariq M

Arthritis & rheumatology (Hoboken, N.J.)

2015

2015-05

OBJECTIVE: Enhanced expression of interleukin-6 (IL-6) plays an important role in the pathogenesis of osteoarthritis (OA). Monocyte chemoattractant protein-induced protein 1 (MCPIP-1) is a novel posttranscriptional regulator of IL-6 expression and is targeted by microRNA-9 (miR-9). We investigated the expression of MCPIP-1 in OA cartilage and explored whether targeting of MCPIP-1 by miR-9 contributes to enhanced IL-6 expression in OA. METHODS: Gene and protein expression in

Academic Medical Centers; Aged; Articular/drug effects/*metabolism; Cartilage; Cells; Chondrocytes – Physiology; Chondrocytes/drug effects/*metabolism; Cultured; Enzyme-Linked Immunosorbent Assay; Funding Source; Gene Knockdown Techniques; Hip/*genetics/metabolism; Human; Humans; Immunoblotting – Utilization; Immunoprecipitation; Interleukin-1beta/pharmacology; Interleukin-6/*genetics/metabolism; Interleukins – Physiology; Messenger/drug effects/*metabolism; MicroRNAs/drug effects/*genetics/metabolism; Middle Aged; Ohio; Osteoarthritis; Osteoarthritis – Complications; Osteoarthritis – Physiopathology; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleases/drug effects/*genetics/metabolism; RNA; T-Tests; Transcription Factors/drug effects/*genetics/metabolism

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