Immunohistochemical localization of substance P, somatostatin, enkephalin, and serotonin in the spinal cord of the northern leopard frog, Rana pipiens.

Title

Immunohistochemical localization of substance P, somatostatin, enkephalin, and serotonin in the spinal cord of the northern leopard frog, Rana pipiens.

Creator

Adli D S; Rosenthal B M; Yuen G L; Ho R H; Cruce W L

Publisher

The Journal of comparative neurology

Date

1988
1988-09

Description

Using the indirect antibody peroxidase-antiperoxidase method of Sternberger, we localized substance P (SP), somatostatin (SOM), enkephalin (ENK), and serotonin (5HT, 5-hydroxytryptamine) in the spinal cord of Rana pipiens. This is the first study to demonstrate all four substances in adjacent sections of frog spinal cord. The distribution patterns of ENK, SP, SOM, and 5HT in our study differ from that described for laminae I and II in amniotes. A high density of ENK, SP, and SOM fibers is present in a band ventral to the dorsal terminal field of cutaneous primary afferent fibers and slightly overlapping the ventral terminal field of muscle primary afferent fibers. However, a high density of 5HT fibers is present in the dorsal terminal field.

Subject

Animals; Enkephalins/analysis; Immunoenzyme Techniques; Male; Neuropeptides/*analysis; Rana pipiens/anatomy & histology/*metabolism; Serotonin/analysis; Somatostatin/analysis; Spinal Cord/*analysis; Substance P/analysis

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

106–116

Issue

1

Volume

275

Citation

Adli D S; Rosenthal B M; Yuen G L; Ho R H; Cruce W L, “Immunohistochemical localization of substance P, somatostatin, enkephalin, and serotonin in the spinal cord of the northern leopard frog, Rana pipiens.,” NEOMED Bibliography Database, accessed September 18, 2021, https://neomed.omeka.net/items/show/2992.

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