Renal Calcium Oxalate Deposits Induce a Pro-Atherosclerotic and Pro-Osteoporotic Response in Mice.

Renal Calcium Oxalate Deposits Induce a Pro-Atherosclerotic and Pro-Osteoporotic Response in Mice.

Kusumi Kirsten; Barr-Beare Evan; Saxena Vijay; Safedi Fayez; Schwaderer Andrew

Journal of cellular biochemistry

2017

2017-09

Urinary stone disease (USD) is increasing in adult and pediatric populations. Adult and pediatric studies have demonstrated decreased bone mineral density and increased fracture rates. USD has also been independently linked to increased rates of myocardial infarction and cerebral vascular accidents. Although USD is a multisystem disorder involving the kidneys, bone, and vasculature, the molecular mechanisms linking these three organs remain unknown. Calcium oxalate nephropathy was induced in C57BL/6J mice with intra-peritoneal (ip) injection of sodium glyoxolate. Half of each kidney underwent Pizzalato staining and half was snap frozen for RNA extraction. RT(2) Profiler Mouse Atherosclerosis, Osteoporosis, and Calcium Signaling PCR Arrays (Qiagen) were performed. Only results that passed quality checks in PCR array reproducibility and genomic DNA contamination were included. Genes had to show at least fourfold differential expression and P \textless 0.01 to be considered significant. Atherosclerosis array showed upregulation of 19 genes by fourfold, 10 of which were \textgreater/=10-fold. All 19 had P 10-fold increase. All 10 have P /=10-fold. All 10 have P

*CARDIOVASCULAR DISEASE; *Gene Expression Regulation; *KIDNEY STONES; *METABOLIC BONE DISEASE; *MURINE MODEL; Animal; Animals; Atherosclerosis/*metabolism/pathology; Calcium Oxalate/*metabolism; Disease Models; Kidney/*metabolism/pathology; Mice; Osteoporosis/*metabolism/pathology; Urinary Calculi/*metabolism/pathology

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