Osteoactivin induces transdifferentiation of C2C12 myoblasts into osteoblasts.

Title

Osteoactivin induces transdifferentiation of C2C12 myoblasts into osteoblasts.

Creator

Sondag Gregory R; Salihoglu Sibel; Lababidi Suzanne L; Crowder Douglas C; Moussa Fouad M; Abdelmagid Samir M; Safadi Fayez F

Publisher

Journal of cellular physiology

Date

2014
2014-07

Description

Osteoactivin (OA) is a novel osteogenic factor important for osteoblast differentiation and function. Previous studies showed that OA stimulates matrix mineralization and transcription of osteoblast specific genes required for differentiation. OA plays a role in wound healing and its expression was shown to increase in post fracture calluses. OA expression was reported in muscle as OA is upregulated in cases of denervation and unloading stress. The regulatory mechanisms of OA in muscle and bone have not yet been determined. In this study, we examined whether OA plays a role in transdifferentiation of C2C12 myoblast into osteoblasts. Infected C2C12 with a retroviral vector overexpressing OA under the CMV promoter were able to transdifferentiate from myoblasts into osteoblasts. Immunofluorescence analysis showed that skeletal muscle marker MF-20 was severely downregulated in cells overexpressing OA and contained significantly less myotubes compared to uninfected control. C2C12 myoblasts overexpressing OA showed an increase in expression of bone specific markers such as alkaline phosphatase and alizarin red staining, and also showed an increase in Runx2 protein expression. We also detected increased levels of phosphorylated focal adhesion kinase (FAK) in C2C12 myoblasts overexpressing OA compared to control. Taken together, our results suggest that OA is able to induce transdifferentiation of myoblasts into osteoblasts through increasing levels of phosphorylated FAK.

Subject

Alkaline Phosphatase/biosynthesis; Animals; Cell Line; Cell Transdifferentiation/*genetics; Core Binding Factor Alpha 1 Subunit/biosynthesis; Developmental/genetics; Eye Proteins/*genetics/metabolism; Focal Adhesion Protein-Tyrosine Kinases/biosynthesis; Gene Expression Regulation; Humans; Membrane Glycoproteins/*genetics/metabolism; Mice; Myoblasts/cytology/*metabolism; Osteoblasts/cytology/*metabolism

Identifier

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

955–966

Issue

7

Volume

229

Citation

Sondag Gregory R; Salihoglu Sibel; Lababidi Suzanne L; Crowder Douglas C; Moussa Fouad M; Abdelmagid Samir M; Safadi Fayez F, “Osteoactivin induces transdifferentiation of C2C12 myoblasts into osteoblasts.,” NEOMED Bibliography Database, accessed June 13, 2021, https://neomed.omeka.net/items/show/3041.

Social Bookmarking