Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.

Title

Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.

Creator

Koo P H; Liebl D J

Publisher

Journal of neuroscience research

Date

1992
1992-04

Description

alpha 2-Macroglobulin (alpha 2M) is a rather ubiquitous protein in extracellular spaces of mammals. It is an inhibitor of endopeptidases, can be modified by aliphatic amines, and combines with a number of hormones/cytokines such as beta-nerve growth factor (NGF) [Koo PH, Stach RW (1989): J Neurosci Res 22:247]. The objective of this study is to compare the NGF-binding properties of methylamine-modified human alpha 2M (MA-alpha 2M) versus normal alpha 2M and their effects on the biological activity of NGF and neurite extension by embryonic chicken dorsal root ganglia. As determined by gel filtration, polyacrylamide gel electrophoresis, and equilibrium binding studies, these two forms of alpha 2M are similar in their binding affinities, with MA-alpha 2M binding about twice as much NGF as normal alpha 2M. Both normal alpha 2M and

Subject

alpha-Macroglobulins/metabolism/*pharmacology; Animals; Cells; Chick Embryo; Cultured; Drug Interactions; Female; Ganglia; Humans; Male; Methylamines/*pharmacology; Mice; Nerve Growth Factors/antagonists & inhibitors/metabolism/*pharmacology; Neurites/drug effects/*physiology/ultrastructure; Neurons/cytology/physiology; Placenta; Pregnancy; Spinal/physiology; Submandibular Gland

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

678–692

Issue

4

Volume

31

Citation

Koo P H; Liebl D J, “Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.,” NEOMED Bibliography Database, accessed June 17, 2021, https://neomed.omeka.net/items/show/3063.

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