Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.

Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.

Koo P H; Liebl D J

Journal of neuroscience research

1992

1992-04

alpha 2-Macroglobulin (alpha 2M) is a rather ubiquitous protein in extracellular spaces of mammals. It is an inhibitor of endopeptidases, can be modified by aliphatic amines, and combines with a number of hormones/cytokines such as beta-nerve growth factor (NGF) [Koo PH, Stach RW (1989): J Neurosci Res 22:247]. The objective of this study is to compare the NGF-binding properties of methylamine-modified human alpha 2M (MA-alpha 2M) versus normal alpha 2M and their effects on the biological activity of NGF and neurite extension by embryonic chicken dorsal root ganglia. As determined by gel filtration, polyacrylamide gel electrophoresis, and equilibrium binding studies, these two forms of alpha 2M are similar in their binding affinities, with MA-alpha 2M binding about twice as much NGF as normal alpha 2M. Both normal alpha 2M and

alpha-Macroglobulins/metabolism/*pharmacology; Animals; Cells; Chick Embryo; Cultured; Drug Interactions; Female; Ganglia; Humans; Male; Methylamines/*pharmacology; Mice; Nerve Growth Factors/antagonists & inhibitors/metabolism/*pharmacology; Neurites/drug effects/*physiology/ultrastructure; Neurons/cytology/physiology; Placenta; Pregnancy; Spinal/physiology; Submandibular Gland

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