The isolation and characterization of the soluble and membrane-bound porcine cytochrome b5 cDNAs.

Title

The isolation and characterization of the soluble and membrane-bound porcine cytochrome b5 cDNAs.

Creator

VanDerMark P K; Steggles A W

Publisher

Biochemical and biophysical research communications

Date

1997
1997-11

Description

In some male pigs, there is an increased production of the testicular 16 androstene steroids which end up being concentrated in fatty tissue. When the meat is cooked, a disagreeable odor/flavor is produced, a phenomenon known as "boar taint." All boars selected for food production are castrated even though only ca 10% of boars may be "tainted." This has a high economic cost because castrated pigs convert food into meat less efficiently, the meat is fatter, and there is an increased mortality due to the castration procedure. Recent data has implicated an increased level of cytochrome b5 in the testes with the increased synthesis of the 16-androstene steroids. As an initial step in analyzing this process, we used 5' and 3' RACE PCR procedures to isolate, clone and sequence the cDNAs for the membrane-bound and soluble forms of porcine cytochrome b5.

Subject

Amino Acid Sequence; Animals; Base Sequence; Blotting; Complementary/blood/*chemistry/*isolation & purification; Cytochromes b5/blood/*chemistry/genetics/*isolation & purification; DNA; Male; Membrane Proteins/*chemistry/genetics/*isolation & purification; Molecular Sequence Data; Solubility; Southern; Swine; Testis/enzymology

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

80–83

Issue

1

Volume

240

Citation

VanDerMark P K; Steggles A W, “The isolation and characterization of the soluble and membrane-bound porcine cytochrome b5 cDNAs.,” NEOMED Bibliography Database, accessed June 19, 2021, https://neomed.omeka.net/items/show/3105.

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