Subcellular localization and antiviral activity of carminic acid/poly r(A-U) combinations.

Subcellular localization and antiviral activity of carminic acid/poly r(A-U) combinations.

Krabill K; Jamison J M; Gilloteaux J; Summers J L

Cell biology international

1993

1993-10

Carminic acid (CAR) enhances the antiviral activity of poly r(A-U) twelve-fold without increasing interferon induction, inactivating the vesicular stomatitis virus or inducing host cell cytotoxicity. Phase contrast photomicrographs of human foreskin fibroblasts (HSF) incubated with CAR alone, poly r(A-U) alone or with a CAR/poly r(A-U) combination illustrate that the CAR/poly r(A-U) combinations display altered subcellular distribution with the CAR being localized in the nucleoli and chromatin. Phase contrast and fluorescence photomicrographs of adriamycin (ADR)-treated and ADR/poly r(A-U)-treated HSF cells corroborate these findings. These results suggest that modulation of one or more nucleolar processes may be responsible for the enhanced antiviral activity.

Antiviral Agents/*pharmacology; Carmine/*analogs & derivatives/pharmacokinetics/pharmacology; Cell Nucleolus/metabolism; Cells; Chromatin/metabolism; Cultured; Doxorubicin/pharmacology; Drug Combinations; Drug Synergism; Fluorescence; Humans; Interferon-beta/biosynthesis/physiology; Microscopy; Phase-Contrast; Poly A-U/*pharmacokinetics/*pharmacology; Vesicular stomatitis Indiana virus/drug effects/growth & development; Viral Plaque Assay

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919–934

10

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