Pertubation of beta1 integrin function using anti-sense or function-blocking antibodies on corneal cells grown on fibronectin and tenascin.

Title

Pertubation of beta1 integrin function using anti-sense or function-blocking antibodies on corneal cells grown on fibronectin and tenascin.

Creator

Doane Kathleen J; Bhattacharya Raka; Marchant Jeff

Publisher

Cell biology international

Date

2002
1905-6

Description

During corneal development, neural crest derivatives from the periocular mesenchyme migrate into the cornea and differentiate into corneal fibroblasts. During this time, these cells interact with a variety of extracellular matrices for proper orientation and development. In the present studies, we have examined the interaction of beta(1) integrins on periocular mesenchyme cells (POM) and corneal fibroblasts (CF) with fibronectin and tenascin by perturbing the function of this integrin. POM and CF attached and spread to a much greater extent on fibronectin than on tenascin. An antibody against beta(1) integrin, CSAT, decreased spreading and attachment, and resulted in a lack of immuno-detectable beta(1) integrin in focal adhesions on fibronectin; few beta(1) positive focal adhesions were observed in cells grown on tenascin. An anti-sense retroviral construct decreased endogenous levels of beta(1) integrin protein, and caused decreased attachment and spreading as well as sparse, disorganized focal adhesions. These data indicate that in vitro, both POM and CF have beta(1) integrins that interact with fibronectin and allow them to attach and spread, while tenascin is anti-adhesive. Further studies using both of these experimental paradigms will clarify whether these interactions also occur in vivo.

Subject

Animals; Antisense/*pharmacology; Cattle; Cell Adhesion; Cell Culture Techniques/*methods; Cells; Chick Embryo; Chickens; Complementary/metabolism; Cornea/*cytology; Cultured; DNA; Dose-Response Relationship; Drug; Fibronectins/*metabolism; Fluorescence; Immunohistochemistry; Integrin beta1/*metabolism/*physiology; Integrins/metabolism; Microscopy; Oligonucleotides; Protein Binding; Retroviridae/genetics; Tenascin/*metabolism

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

131–144

Issue

2

Volume

26

Citation

Doane Kathleen J; Bhattacharya Raka; Marchant Jeff, “Pertubation of beta1 integrin function using anti-sense or function-blocking antibodies on corneal cells grown on fibronectin and tenascin.,” NEOMED Bibliography Database, accessed June 19, 2021, https://neomed.omeka.net/items/show/3110.

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