Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.
Title
Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.
Creator
Crish Samuel D; Schofield Brett R
Publisher
Methods in molecular biology (Clifton, N.J.)
Date
2018
1905-07
Description
Whether to stage degeneration or investigate early pathology in glaucoma, examination of axonal structure and function is essential. There are a wide variety of methods available to investigators using animal models of glaucoma, with varying utilities depending on the questions asked. Here, we describe the use of anterograde neuronal tract tracing using cholera toxin B (CTB) for the determination of axon transport integrity of the retinofugal projection. This method reveals the structure of the retinal axons as well as the functional integrity of anterograde transport systems.
Subject
*Axonal transport; *Axonopathy; *Neuronal tracing; *Optic nerve; *Superior colliculus; Animal; Animals; Axonal Transport; Axons/metabolism/*pathology/physiology; Cholera Toxin/*metabolism; Confocal; Disease Models; Glaucoma/*diagnostic imaging/metabolism/physiopathology; Humans; Mice; Microscopy; Rats; Visual Pathways
Identifier
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Citation
Crish Samuel D; Schofield Brett R, “Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.,” NEOMED Bibliography Database, accessed November 12, 2024, https://neomed.omeka.net/items/show/3132.