Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.

Title

Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.

Creator

Crish Samuel D; Schofield Brett R

Publisher

Methods in molecular biology (Clifton, N.J.)

Date

2018
1905-07

Description

Whether to stage degeneration or investigate early pathology in glaucoma, examination of axonal structure and function is essential. There are a wide variety of methods available to investigators using animal models of glaucoma, with varying utilities depending on the questions asked. Here, we describe the use of anterograde neuronal tract tracing using cholera toxin B (CTB) for the determination of axon transport integrity of the retinofugal projection. This method reveals the structure of the retinal axons as well as the functional integrity of anterograde transport systems.

Subject

*Axonal transport; *Axonopathy; *Neuronal tracing; *Optic nerve; *Superior colliculus; Animal; Animals; Axonal Transport; Axons/metabolism/*pathology/physiology; Cholera Toxin/*metabolism; Confocal; Disease Models; Glaucoma/*diagnostic imaging/metabolism/physiopathology; Humans; Mice; Microscopy; Rats; Visual Pathways

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

171–185

Volume

1695

Citation

Crish Samuel D; Schofield Brett R, “Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.,” NEOMED Bibliography Database, accessed January 23, 2021, https://neomed.omeka.net/items/show/3132.

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