Injury models to study cardiac remodeling in the mouse: myocardial infarction and ischemia-reperfusion.

Title

Injury models to study cardiac remodeling in the mouse: myocardial infarction and ischemia-reperfusion.

Creator

Luther Daniel J; Thodeti Charles K; Meszaros J Gary

Publisher

Methods in molecular biology (Clifton, N.J.)

Date

2013
1905-07

Description

Deep tissue wound healing requires a complex sequence of several factors working in unison to repair the organ at risk. Myocardial infarction (MI) is particularly complex due to several local and systemic factors mediating the repair process within the heart. The wound healing process during this time is critical-the cardiac myocytes are at risk of apoptotic cell death, autophagy, and necrosis. During the early remodeling period, the fibroblasts and myofibroblasts play critical roles in infarct scar formation, a process that is greatly influenced by a robust inflammatory response. Construction of the infarct scar is a "necessary evil" that helps to limit expansion of the infarction; however, the collagen and matrix deposition will often spread to the healthy areas of the heart, causing reactive fibrosis in areas remote from the original damage. This chapter outlines in detail the procedures for two myocardial infarction injury models as well as how to quantify the size of the experimentally induced injury. These procedures are critical to the development of in vivo approaches to study myocardial injury, particularly for use in knockout and transgenic mice.

Subject

*Ventricular Remodeling; Animal; Animals; Cardiovascular Surgical Procedures; Coronary Vessels/surgery; Disease Models; Ligation; Mice; Myocardial Infarction/*etiology/*pathology; Myocardial Reperfusion Injury/*etiology/*pathology; Perfusion/methods; Wound Healing

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

325–342

Volume

1037

Citation

Luther Daniel J; Thodeti Charles K; Meszaros J Gary, “Injury models to study cardiac remodeling in the mouse: myocardial infarction and ischemia-reperfusion.,” NEOMED Bibliography Database, accessed June 17, 2021, https://neomed.omeka.net/items/show/3133.

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