The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.

Title

The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.

Creator

Giordano S J; Yoo M; Ward D C; Bhatt M; Overhauser J; Steggles A W

Publisher

Human genetics

Date

1993
1993-12

Description

Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.

Subject

*Chromosomes; Animals; Blotting; Cell Line; Chromosome Mapping; Cricetinae; Cytochromes b5/*genetics; DNA/analysis; Genetic/genetics; Human; Humans; Hybrid Cells; In Situ Hybridization; Molecular Sequence Data; Pair 14; Pair 18; Pair 20; Polymerase Chain Reaction; Pseudogenes/*genetics; Southern; Translocation

Identifier

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

615–618

Issue

6

Volume

92

Citation

Giordano S J; Yoo M; Ward D C; Bhatt M; Overhauser J; Steggles A W, “The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.,” NEOMED Bibliography Database, accessed September 21, 2021, https://neomed.omeka.net/items/show/3141.

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